LOCUS       sequence-238204-       12755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemOrange2) fused to a lysosomal localized gene (cup-5) under the 
            intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 12755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pBT3011
FEATURES             Location/Qualifiers
     source          1..12755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10329..10351)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10451..10470
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10548..10566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10634..10738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10739..10807,10808..11599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10957..10976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11770..12358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12259..12278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12512..12529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12646..12667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12682..12687,12688..12705,12706..12712)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12720..12736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12725..12747
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(12744..12755,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(12744..12755,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttccttcca agttattgac aagtattaac tgcacttctc
       61 ataaaaattt ttcaaataga tctatttagt ctggaatagg aatacttttg gcaattttct
      121 tgagaatcat agaagacgaa tttctgaact gctctttgtg aagccacgcc catatattgg
      181 catttttgta tccaattttt tgtagagtac ctgaatcatt gtcactacat ctgctaaaca
      241 atatatggaa gttctatgag aggtgaaagg taaatcatat aacaactgta ataacataga
      301 aatgattgta gtatatttcc aattaggctg agttgatctt attgaagagt ttgaatcata
      361 gcaatttttt cctcttttag tatttttcaa aacaaatttt cacaatgtta gatgtagtat
      421 ttagcattaa ggcatctgta atctcccaga aatgttttgg gaataaaacc ctttgactct
      481 ttggtccttt tttgctccaa ataatatctg attttaaccc gttctgatct actttttacc
      541 tttcgatcgt cacatccgcg tacacgtttc tcttgctctc aattgcttat ctttatggcc
      601 ttttccccca ctttttgtcg actatcatgc ggacgtggct tccttgctcc ctcccggttt
      661 ttttgaaaac acttgtgaat ttattttttg tggtcaaaat tacgaaacat ttgaattctc
      721 aatctattta tagaccgggt gtggtatgtt atgccatgtt tttgtcattg ccaaatgaac
      781 atcgatattt taattttctg aacttaccgt accccctgcg ttactatttt atttttaatt
      841 tgtataattt cattgctata atttgttgtc cttgaaacat tgaagatatc gaagaaataa
      901 tgacaaaaat cagatagaac aatttttcct actcatcaac tcacattttt ctattttctc
      961 ttgtgcgaaa aaaatagtat attgggaata tttatagtat tgcaactttt ctcttagttc
     1021 gcagagttgc aatactacta gatccctaat agaggaaata tggtgaccag ctttttgcaa
     1081 ttggaaagtt gggaaaaaag tatatatatt aatagactta cattcttgta gttcgtagaa
     1141 tttttcagga aatctgacac gcaagacttt ttcaaaaagt tttttttttt aatttggcaa
     1201 tctgccaaaa cgtttactga acattttgaa aacatctacc agtaccttta acacaacgaa
     1261 aaattgttct aagcaatatt aactgtgata attgatgtta agacctgaaa tttaatcaca
     1321 aatttcacct ggaaatgaca gtaatcggct tctaaatact gtcgacacaa gtgccatacc
     1381 taagattcca atcctattac catgtttccc aatttcattc tctttcgtat ctctatttct
     1441 cttctttatt atcaattact ttttatcagt tcttcgtttg ctttaacatc aagagcacat
     1501 agcgctctct cgtctctctc tcatgctctt tttgaacttt tacaaaaaac tatttccggt
     1561 tgttttgatt cttggaattt gaaataattt tcagtgatta aatctagagg atccccggga
     1621 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1681 ggaggaccct tggctagcct cgagaggcct ggtaccatgg gcggaaaaat ggtctccaag
     1741 ggagaggaga acaacatggc catcatcaag gagttcatgc gtttcaaggt ccgtatggag
     1801 ggatccgtca acggacacga gttcgagatc gagggagagg gagagggacg tccatacgag
     1861 ggattccaaa ccgccaagct caaggtcacc aaggtaagtt taaacatata tatactaact
     1921 aaccctgatt atttaaattt tcagggagga ccactcccat tcgcctggga catcctctcc
     1981 ccacacttca cctacggatc caaggcctac gtcaagcacc cagccgacat cccagactac
     2041 ttcaagctct ccttcccaga gggattcaag tgggagcgtg tcatgaacta cgaggacgga
     2101 ggagtcgtca ccgtcaccca agactcctcc ctccaagacg gagagttcat ctacaaggta
     2161 agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggt caagctccgt
     2221 ggaaccaact tcccatccga cggaccagtc atgcaaaaga agaccatggg atgggaggcc
     2281 tcctccgagc gtatgtaccc agaggacgga gccctcaagg gaaagatcaa gatgcgtctc
     2341 aagctcaagg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     2401 gacggaggac actacacctc cgaggtcaag accacctaca aggccaagaa gccagtccaa
     2461 ctcccaggag cctacatcgt cgacatcaag ctcgacatca cctcccacaa cgaggactac
     2521 accatcgtcg agcaatacga gcgtgccgag ggacgtcact ccaccggagg aatggacgaa
     2581 ctctacaagc ttaacggtcc taggagatct gaattctctc gccggagcac aactgatcgt
     2641 tcagacttca acgataatgc ttcgaatgct tcttcaaatg cttctcgaag gccaacaata
     2701 aattttcaag aaataatgga agatcttccg caccacgaaa gagaaaccgg cgaacgtctt
     2761 cgccgtcatc tccaattctt ttttatgaat ccaatggaga aatggaaagt cagacatcaa
     2821 ctgccctata agttggttct acaagtgctc aaaatagttt tcgttaccat gcaattaatt
     2881 ttattcgcgg aaatgcgcat gtctcatgtt gattttctcg aagacaccac aacagttatg
     2941 agacatcgat ttttaaaaga atggaatgac gacagagatg cattacaata tccaccagct
     3001 gaaggaagat attcggttta tgatgatcaa ggattatccg agcatttgag ctttttgatt
     3061 aattctgtaa gtcgctttgg agaactatct tgtaaaatga tcaatattgt ttcagtatta
     3121 ttcaattcgc aatgattcat ttgcttcatt ttcctatgac gttgtaagtc atccatctgg
     3181 aaatcttggc gctcaaatta gttttgaatc tattccacct attgaagttc ttatcgatcg
     3241 aatttccaac gtaacagtga acaataacac ttataatttc gacattcgtg aagtgaaagg
     3301 tatataaaca tactattatg aaataaaaat agtttatttc tagacacgaa acgtctgaat
     3361 ctaacagaaa cggaagtctt ccaaatcgga caatccgatg atgctgttcg agatattctc
     3421 gccactcgtg gaatcacatt tctcccagaa gacgcgctca agatatcgac ggtccaattc
     3481 aaatttcgac tacgaacaat tcattattca ccaacagccg gagatcagaa accagaatgc
     3541 tataagatat cagtttctat caaatttgat aattcaaggc atacaggaca ggttcatgtc
     3601 actctatcaa ctgtggtttc ttatgtgaat gtttgtaatg gaaggatcat caaaggtgaa
     3661 cacttcactt ttttcaaatt ttcaaagtaa agctagtaaa aattatagat tatgtaatcg
     3721 aaaattaccc aattaacata gaattccaat aattaatggt gaagaatcat caaatgggat
     3781 gctattaaat ataacttcaa atcttttata aaagtctttc tgaaacttcg tgttaaaatc
     3841 tacataactt tatttgccta aaaaagttct tcttgagagt aacatttaga ttttaggtat
     3901 tcaaaaattt cccaattaac ttgaaatatt taacttctag gtgtacttct gtgtttaaat
     3961 aaaaatgaat tttgtaatat taaaatatca aaatataaaa attcaatcaa tctcgagtta
     4021 ggtcaagttt caggtgttgg atggtcattt gacacacttt taatcggcgg aaccgatatt
     4081 ttcgttctca ttctttgcat tctatcactg attctctgct gccgagccct catcaaagcc
     4141 catctacttc aaatcaaaac aagcgactat tttgagaatg tactcaaaaa taagatcacc
     4201 gttaccgatc aactcgattt tctgaatttg tggtatgtga tgattgtggt caacgatgcg
     4261 cttattatca ttggaactgt tgcaaaaata tccatcgagt ttcaggtaat ttttataaaa
     4321 atttaagttt aattcaaatg aaaaattccc tccaatactc aaacataaaa tatattaaat
     4381 ttagttcaac aaaaatactt ttactacaag aaatgtgagt aaaaatttat atttccaata
     4441 acttcctttc tgtatctgtc cgtcccattg tttccatcaa cttttcatcc cgggatttat
     4501 cgaaaccaaa ttgcccctct gtattgatta gaaaattatt ttccattaat tcttgcataa
     4561 aactaagatt attgctttct tttcaggatt tcgacaattc cctcttcacg ctaacttcga
     4621 ttttcctcgg aatgggagct ttgctggtat atgtcggagt tcttcgatat tttggatttt
     4681 tcagtcaata taatgtgagt tgtttcaaat tgctttattt cattacaaca acatttgatc
     4741 gtgttggaat accaactccg gatatggaat tacttgttat tgtagtctta tgcgagtgta
     4801 ctgtagtgta atacattatc caaggtgggc aacatatgga aatatccagt gcgaatgcac
     4861 gtagatcttg acatgtttca cgaatgaatg acgaagtacc agtgtagatc attatcagag
     4921 cctataaaaa atttttaaaa aagtaaatac tccaataatt cattatttcc ttacctgtaa
     4981 taaagcctgc agtaccagtg aaaaacatac cactaacgct atagaaaaca ttgcaatttc
     5041 tactcgtcgt gttctttttc gatgaaactt aaaattgatt atgcttctaa tataatatta
     5101 ttcaaactaa cttcgttctg agtaccacta gccgttttta ctgtttgaat tgtcgttttg
     5161 tacttaacaa ttgttgtgaa attcgctgca acacatatta cggcgagaag aatcgacaca
     5221 aaacacagcg ccgtactgtt tctaggctgt aattttattg attttcatat tttgtgtttg
     5281 gtggaaacca taccaaaaat cctatcaaca gtttcactct gtataccatt tgataattgg
     5341 atcgattttc gtatagtggt aagaaataag cgtcataggt gactaagtgc catgtagtaa
     5401 ttaatggaag aatgtagaca agtgcgattg cactgcggac gttcttttgc caaaacttta
     5461 attttttagg agattcatat ttttcggatg ggaaggctca ccgaattaca gtaatacggg
     5521 aaaagtacag aagtcattcg attgatgcat attaatgtta ttgaataaaa gttcacatag
     5581 atcaagtaat acgagagaaa gtagattata gtcataggta ttccttctgg gatgtatgaa
     5641 ggaagatttt caaaaagacc agaaattgga agacgtgtaa atagaaagtc gattataaaa
     5701 agtgcaacat tctgaaaaag tttacacttt ttatccgaaa ggtctatgta cttacaatat
     5761 agaaatcatt tgtgaaaatc acattgaaat agctgctagt gacgctttcc ttcgtcaaaa
     5821 acgtgcggca tttatgatat gctataaatg atggaacagt gtagagtaga tgaattagaa
     5881 gtagaggttg tctggaaatc tcgattacat tttaggcgcg aaatgttgaa atttttgctg
     5941 gtatataata aaaaaaaagc ggatgaatgt ccagaattcg attaagctcc ttattttcgt
     6001 gaaatttttt gcttaattta cgtttaaaat atgttcaaat aaacttacct ataatcaaac
     6061 gcatatgcca catttctaat ttcaccatca gatactgaaa ctactgtttc gtttccatgc
     6121 gagaaaatgg aactcattcg aaaaaattgt tcgaaatgaa gaagacaaag aaaaaggaaa
     6181 agagctcaat ggtcttcaaa tttaattgaa aagaccaccg accaatagaa tgttgccctg
     6241 caaacaacaa gttttaaact tgtgaaaatt ggggaaatta aggggagtag atggaaggat
     6301 ccatttacat taacaagcta aagcatacta caaacattac aaaattaagg attggcgtat
     6361 gtatatatat atattgaaag cttcgaactg gaaaacttcg acttgattag acgacgtcta
     6421 acaacgcgtg attaagattt tgttgttcat agaaactgag ttaattaatc acgcattgtc
     6481 tagaatgatg tcaaagtgta tttgatgtga aaagtgcaaa tgtaaatgaa aattctccag
     6541 agattaaata tttctttaat ttgatattgt ttcactggtg cccccaaaag tatcacgttt
     6601 gagagaagga aaagtttaaa agagaagtca aaaagccgta tctaaaacct tggcacaact
     6661 aaaataaaga gggtcttatg tcaaaacata aatcaatttt catacctctt ttgtttcttc
     6721 ctggtatctt gaaaattgga aacgagaaaa tgatgtcaac aataccgaat ttttaaatat
     6781 tcttaactct ttcttttaag agaaaatctt tttctgaaag tttacaaaaa tgaatccgaa
     6841 tttttattca aatttttgct gttggtgaga aaaacatgat taaaataatt tcaaaaaatg
     6901 agaataattg ccaaaaaaaa aatttgatag ttgctcttcg attatccgca agatataaat
     6961 ttgaaaattc caaaaaaatt aaaaattatt tttaaatgtt gtagtgagta gttctagcta
     7021 atttccgaaa aaaggaaaat ataaaataga aaaaaaacca ttttaatttt ataaaaattc
     7081 gggaaaattc ttgaaattta cgttctttaa tttttcagat aaaatttaaa aagcaagatc
     7141 ctatgttaaa taattggcac aatctagaac aagccagttc tagattgagc caattattta
     7201 acaaaatcac tctataagta aaaataaccg caatcaaaga gaaaagctaa atataatgga
     7261 atgattaaaa tactaaaaaa acttttaaaa aagcttctgc tggtttcaaa cgataaaagt
     7321 gaaggaaaaa tgtttatttt tgccattttc cagatcctca tgctcacgct caaacgatcg
     7381 gcacccaata tcatgcgctt catgacgtgt gccattgtac tttacgctgg tttcttgatt
     7441 gccggatggg ttattattgg cccgtacagt atgaagttcc gtacactggc agaatcatcg
     7501 gaagctctat tttcgcttct caacggtgac gatatgttcg ccacgtttta cactattaat
     7561 gattccaata cagttatcaa ggtaaagatt tttctttttt ttttctagat tatcttaaat
     7621 atttcaggta tttgggacag tgtacatcta cctattcgtg tcactcttta tctatgtagt
     7681 attatcactg ttcattgcaa ttatcatgga tgcatatgaa gtcgtcaagg taataatgaa
     7741 cagagcaaat gaacatgaaa atgagaacaa aaagctcgaa aaaacaactg aagaaagagc
     7801 aatagaccac gccaatggga tgctgtttga gtgttgatga aaattggaaa aatgaaaaca
     7861 acaagaaagc aatagctctt cattttcctt gcccatccat ctatttttaa ttattgcggc
     7921 gaaaaatgaa agatgatcaa gtgcttactc gcagctaaaa atgaaattct atgtcagaag
     7981 acaaggagac tagtcaaatg tcagatttcg gtgtaaccaa tgatttagtt tttgtcttgc
     8041 cgaggtctga aagagaaagc atagacacca ccagtctcaa attatttttt ttttttgaga
     8101 attcatcatt ctgtaactag tgaaactcga aattggctca aagaaatgag aacccaccct
     8161 ttttgacata cgtattatag gtttgcagaa aatttgactt ttgaaattta ggtgcaagtg
     8221 cgcatcaatc acttttcaac ttcacatgca tttatttaaa aaaaaaacat aatttcgact
     8281 tgaaaaactg aaaaaaataa aaaaaataac gaaatttttt tcaaatttct actgaattta
     8341 cggtctggtc agggcgggga attttttacg cgcctccata atattaacca tgttaatgaa
     8401 tttttaaatt taagaaataa caaaacggta aaagttaata attatatctc attcattcca
     8461 ggatcgctac tcagacggtc ttcgagccat cgaaaaacgt ggatgtcttc gcgatttcgt
     8521 ggaatcgaat ccaccgccgt cggaactcgg ctcaccgacg actcgatccg cctacgcacc
     8581 gtctaatctg ctcaatctgg gtcggtgcgt gcctgttgaa gtgacacgaa tctttctctg
     8641 ttcaggcttt ctacacactt tttcattttt cttacattta tgcacgccaa acaaacttgg
     8701 ttgctagttt tggtgatggt gttgtgacag cagcaactaa cagcgtcgtg tagtggtttg
     8761 acagtactaa aacaatcaca aaagcattaa aacaattaaa atatagaata acagtctcta
     8821 gattacagtt agcatagtag cacgtacaaa aaactaacta gatattgcag ttgttcatca
     8881 gttcgttcga aaacattcag tttctctaat ttcagcggct ggcaacgact cgagtagagc
     8941 tcttcgtgct cttcatgcca ttgacaatgc tcgcgattgg ctgagcagct taagagatgg
     9001 aactcggtaa ttttaatttt aaaagtgtat taggagggct tgtttcgttt tgcaagtttt
     9061 cgactaaaat ctactaaaaa ttttcgatac tctattaact gaacgagata atttgaaaat
     9121 tgataaataa tccctctggt ttttcagttt tcaatcattc tccaacccgc tgaacgactc
     9181 aaatgaagat gtattagccg accaaaatgc caatggaaac gatcctcaca attctaggcc
     9241 atcgaatgta tagagtttct caatttaatt ttcttttgtt atcaaaaaca actttgtctg
     9301 tatttttggt catcttcatt catcttccat cccatccgtt tctttctcct tcctctttca
     9361 tatttattcc cctctgaaca acctacttat catgtgtata tgtggtgcta tgtacttttg
     9421 tgatattctt cttccgtttt gttcgtgatt tataatttgt gatttcttga gaacttaata
     9481 aattgtaatt tattcaaata aggcgcctaa gagctccgca tcggccgctg tcatcagatc
     9541 gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     9601 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
     9661 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     9721 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     9781 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     9841 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     9901 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     9961 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
    10021 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
    10081 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
    10141 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
    10201 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
    10261 ataactgttt ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac
    10321 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10381 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    10441 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    10501 ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg
    10561 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
    10621 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10681 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10741 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10801 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10861 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10921 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10981 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    11041 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    11101 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    11161 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    11221 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    11281 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    11341 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    11401 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11461 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11521 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11581 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11641 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11701 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11821 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11881 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11941 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    12001 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    12061 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    12121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    12181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    12481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    12541 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    12601 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    12661 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    12721 tgtgagcgga taacaatttc acacaggaaa cagct
//
