LOCUS       sequence-238206-         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins 
            via trimeric coiled-coil.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, 
            Howarth M
  TITLE     Spy
  JOURNAL   Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w.
  PUBMED    30988307
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-04-15"; volume: "10(1):1734. doi"; pages: " 
            10.1038/s41467-019-09678-w"
COMMENT     Sequence Label: SpyCatcher002-oTri
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             79..417
                     /codon_start=1
                     /product="improved SpyCatcher variant that shows rapid 
                     reaction with SpyTag002 (Keeble et al., 2017)"
                     /label=SpyCatcher002
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     EATKGDAHT"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggt aaccacctta tcaggtttat caggtgagca aggtccgtcc
      121 ggtgatatga caactgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
      181 gacggccgtg agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
      241 agtacatgga tttcagatgg acatgtgaag gatttctacc tgtatccagg aaaatataca
      301 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt
      361 aatgagcaag gtcaggttac tgtaaatggc gaagcaacta aaggtgacgc tcatactgga
      421 tccagtggta gcggcagcgg ctctgaaatt gcagcaatca aaaaagaaat cgcagccatc
      481 aagcaagaga ttgccgcaat taaacaagag atagcggcta ttaagaaaga aatagctgcc
      541 attaaacagc agggcagtgg ttcgggcgaa ccggaagcgt aataa
//
