LOCUS       sequence-238214-        8001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under 
            the intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pBT3035
FEATURES             Location/Qualifiers
     source          1..8001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      62..650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     551..570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     804..821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    938..959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(974..979,980..997,998..1004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1012..1028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1017..1039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1036..1052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1036..1052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6622..6644)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6744..6763
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6841..6859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6927..7031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7032..7100,7101..7892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7250..7269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
       61 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      121 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      181 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      241 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      301 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      361 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      421 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      481 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      541 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      601 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      661 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      721 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      781 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      841 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      901 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      961 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1021 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt ccttccaagt
     1081 tattgacaag tattaactgc acttctcata aaaatttttc aaatagatct atttagtctg
     1141 gaataggaat acttttggca attttcttga gaatcataga agacgaattt ctgaactgct
     1201 ctttgtgaag ccacgcccat atattggcat ttttgtatcc aattttttgt agagtacctg
     1261 aatcattgtc actacatctg ctaaacaata tatggaagtt ctatgagagg tgaaaggtaa
     1321 atcatataac aactgtaata acatagaaat gattgtagta tatttccaat taggctgagt
     1381 tgatcttatt gaagagtttg aatcatagca attttttcct cttttagtat ttttcaaaac
     1441 aaattttcac aatgttagat gtagtattta gcattaaggc atctgtaatc tcccagaaat
     1501 gttttgggaa taaaaccctt tgactctttg gtcctttttt gctccaaata atatctgatt
     1561 ttaacccgtt ctgatctact ttttaccttt cgatcgtcac atccgcgtac acgtttctct
     1621 tgctctcaat tgcttatctt tatggccttt tcccccactt tttgtcgact atcatgcgga
     1681 cgtggcttcc ttgctccctc ccggtttttt tgaaaacact tgtgaattta ttttttgtgg
     1741 tcaaaattac gaaacatttg aattctcaat ctatttatag accgggtgtg gtatgttatg
     1801 ccatgttttt gtcattgcca aatgaacatc gatattttaa ttttctgaac ttaccgtacc
     1861 ccctgcgtta ctattttatt tttaatttgt ataatttcat tgctataatt tgttgtcctt
     1921 gaaacattga agatatcgaa gaaataatga caaaaatcag atagaacaat ttttcctact
     1981 catcaactca catttttcta ttttctcttg tgcgaaaaaa atagtatatt gggaatattt
     2041 atagtattgc aacttttctc ttagttcgca gagttgcaat actactagat ccctaataga
     2101 ggaaatatgg tgaccagctt tttgcaattg gaaagttggg aaaaaagtat atatattaat
     2161 agacttacat tcttgtagtt cgtagaattt ttcaggaaat ctgacacgca agactttttc
     2221 aaaaagtttt tttttttaat ttggcaatct gccaaaacgt ttactgaaca ttttgaaaac
     2281 atctaccagt acctttaaca caacgaaaaa ttgttctaag caatattaac tgtgataatt
     2341 gatgttaaga cctgaaattt aatcacaaat ttcacctgga aatgacagta atcggcttct
     2401 aaatactgtc gacacaagtg ccatacctaa gattccaatc ctattaccat gtttcccaat
     2461 ttcattctct ttcgtatctc tatttctctt ctttattatc aattactttt tatcagttct
     2521 tcgtttgctt taacatcaag agcacatagc gctctctcgt ctctctctca tgctcttttt
     2581 gaacttttac aaaaaactat ttccggttgt tttgattctt ggaatttgaa ataattttca
     2641 gtgattaaat ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     2701 atactaacat aacatagaac attttcagga ggacccttgg aaaaatgtca tctcgtaaag
     2761 gtactcgtag ttctcgtatt gttacgctag agcgctcagc gaattcgtcg ctaagcaaca
     2821 atggaggagg cgacgattca ttgtgagttt ttattttgct tctctagttc ctgcactcat
     2881 aaacgcattt cttgtgttat ctaaaaaaaa actcgatgaa atgcttttca gtggctcaac
     2941 gcttctagaa acttcacgtc ttcaagagaa agatcatttg acttcactca acagtcgtct
     3001 tgccacttac atcgacgtga gttttaattt ggaaagtatt catatttgaa agtacacttt
     3061 tcagaaagtt cgtcaattgg agcaagagaa caacagactc caggttcaaa ttcgcgacat
     3121 cgaagttgtt gaaaagaaag agaagtcaaa cttggccgat cgcttcgagg cggaaaaggt
     3181 acactttgtt tatattctga tcgccaaagg tgttctgtgg gaaactgatg cggtggtctt
     3241 attattctcg ccgcggtaaa gtaacaatgc aaatataagc tctcgttttg gacgaatgat
     3301 aagtgttgga atatagatta tttgtatttt caggctcgtc tccgtcgtgc cctcgattcg
     3361 gctcaagatg agctcgcaaa atacaggatc gagtatgacg ctgcaaaggt tgaagtaaag
     3421 aagttgaagc cacaagtcga aaaacttgag agagaactcg ctggagctga ggaacaagcc
     3481 ctccatgccc aatctattgc tgatcaaagt caagcaaaac agaagacgtt gcaggcacgc
     3541 aacgataaat tggtggtgga gaatgatgta agaaatttta attttatcaa aagtaaatgt
     3601 tcaattttgt tgttttcagg atctcaaaaa gcagaacatc actcttcgtg acaccgtaga
     3661 aggactcaag aaagccgttg aagatgaaac tcttctccga acagccgcca acaataaaat
     3721 caaggctctg gaagaagatc tcgcttttgc tcttcaacag cacaagggag aacttgaaga
     3781 agttcgtcac aagagacagg tcgacatgac aacctacgcc aagcagatta atgatgagta
     3841 tcaatctaag cttcaagatc aaatcgaaga gatgcgtgct cagttcaaga acaatttgca
     3901 tcaaaacaaa acagctttcg aagatgccta caaaaacaag ctcaatgctg ctcgtgaacg
     3961 ccaagaggag gctgtatccg aagcaatcca tcttcgtgcc cgtgttcgtg acttggagac
     4021 atcaagcagt ggaaatgctt cgctcatcga acgtcttcgt tcagagctcg acactctgaa
     4081 gagatcgttc caagagaagc tcgacgacaa ggatgctcga attgctgaac ttaatcaaga
     4141 gatcgagcgc atgatgagcg agttccacga tcttcttgat gttaaaatcc aattggacgc
     4201 cgaactcaag acctaccaag ctctccttga gggtgaggag gagcgtctca atcttactca
     4261 ggaggcgcca caaaacactt cagttcatca cgtctcgttt tcatccggag gagcaagcgc
     4321 tcagcgcgga gtgaagcgtc gtcgcgttgt cgatgtaaat ggagaggacc aagacattga
     4381 ttatctcaac cgtcgctcca aactcaacaa agagactgtt ggcccagttg gaatcgacga
     4441 ggttgatgag gaaggaaagt gggtccgtgt tgcaaacaac tctgaagaag aacaatccat
     4501 cggaggatac aagttggtgg tcaaagctgg aaacaaagaa gcctccttcc aattttcatc
     4561 tcgtatgaag ctcgctccac atgctagcgc caccgtttgg tctgcggatg ctggtgccgt
     4621 tcaccaccca ccagaagtct acgttatgaa gaagcaacag gttggtcaat tattttaaaa
     4681 tatttctgtc ttactttcat gaatagaaga aacaaaaaat gttcattgta attacaattt
     4741 tcagtggcca attggagata acccatcagc tcgtcttgag gatagtgaag gagacactgt
     4801 ttcttctatc accgttgaat tcagcgaatc atcggatcca tcggacccag ccgatcgttg
     4861 ttccatcatg gctagcctcg agaggcctgg taccatgggc ggaaaaatgg tctccaaggg
     4921 agaggagaac aacatggcca tcatcaagga gttcatgcgt ttcaaggtcc gtatggaggg
     4981 atccgtcaac ggacacgagt tcgagatcga gggagaggga gagggacgtc catacgaggg
     5041 attccaaacc gccaagctca aggtcaccaa ggtaagttta aacatatata tactaactaa
     5101 ccctgattat ttaaattttc agggaggacc actcccattc gcctgggaca tcctctcccc
     5161 acacttcacc tacggatcca aggcctacgt caagcaccca gccgacatcc cagactactt
     5221 caagctctcc ttcccagagg gattcaagtg ggagcgtgtc atgaactacg aggacggagg
     5281 agtcgtcacc gtcacccaag actcctccct ccaagacgga gagttcatct acaaggtaag
     5341 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtca agctccgtgg
     5401 aaccaacttc ccatccgacg gaccagtcat gcaaaagaag accatgggat gggaggcctc
     5461 ctccgagcgt atgtacccag aggacggagc cctcaaggga aagatcaaga tgcgtctcaa
     5521 gctcaaggta agtttaaaca tgattttact aactaactaa tctgatttaa attttcagga
     5581 cggaggacac tacacctccg aggtcaagac cacctacaag gccaagaagc cagtccaact
     5641 cccaggagcc tacatcgtcg acatcaagct cgacatcacc tcccacaacg aggactacac
     5701 catcgtcgag caatacgagc gtgccgaggg acgtcactcc accggaggaa tggacgaact
     5761 ctacaagctt aacggtccta ggagatctga attcggcgcc taagagctcc gcatcggccg
     5821 ctgtcatcag atcgccatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
     5881 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
     5941 acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     6001 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     6061 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     6121 ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta
     6181 tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct
     6241 catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat
     6301 caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt
     6361 ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
     6421 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
     6481 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
     6541 caatatacca aacataactg tttctactag tcggccgtac gggccctttc gtctcgcgcg
     6601 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6661 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6721 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     6781 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
     6841 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6901 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6961 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7021 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7081 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7141 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7201 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7261 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7321 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7381 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7441 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7501 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7561 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7621 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7681 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7741 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7801 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7861 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7921 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7981 ataatctcat gaccaaaatc c
//
