LOCUS       sequence-238222-        4098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses the phosphopantheteine transferase Sfp of Bacillus 
            subtilis to transfer a PPant arm to acyl carrier proteins in 
            Escherichia coli. Untagged; in pCDF-1b vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M
  TITLE     Probing the modularity of megasynthases by rational engineering of a
            fatty acid synthase Type I.
  JOURNAL   Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 
            2018 Dec 20.
  PUBMED    30394635
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3550"; 
            journalName: "Protein Sci"; date: "2019-02"; volume: "28"; issue: 
            "2"; pages: "414-428"
COMMENT     Sequence Label: pAR357_SFP_pCDF-1b
FEATURES             Location/Qualifiers
     source          1..4098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    47..71
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(809..827)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      823..870
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1041..1832)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1833..1924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1972..2710)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(2920..4002)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3964..3983
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4003..4080)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ccttatgcga ctcctgcatt aggaaattaa tacgactcac tataggggaa ttgtgagcgg
       61 ataacaattc ccctgtagaa ataattttgt ttaactttaa taaggagata taccatgaag
      121 atttacggaa tttatatgga ccgcccgctt tcacaggaag aaaatgaacg gttcatgact
      181 ttcatatcac ctgaaaaacg ggagaaatgc cggagatttt atcataaaga agatgctcac
      241 cgcaccctgc tgggagatgt gctcgttcgc tcagtcataa gcaggcagta tcagttggac
      301 aaatccgata tccgctttag cacgcaggaa tacgggaagc cgtgcatccc tgatcttccc
      361 gacgctcatt tcaacatttc tcactccggc cgctgggtca ttggtgcgtt tgattcacag
      421 ccgatcggca tagatatcga aaaaacgaaa ccgatcagcc ttgagatcgc caagcgcttc
      481 ttttcaaaaa cagagtacag cgacctttta gcaaaagaca aggacgagca gacagactat
      541 ttttatcatc tatggtcaat gaaagaaagc tttatcaaac aggaaggcaa aggcttatcg
      601 cttccgcttg attccttttc agtgcgcctg catcaggacg gacaagtatc cattgagctt
      661 ccggacagcc attccccatg ctatatcaaa acgtatgagg tcgatcccgg ctacaaaatg
      721 gctgtatgcg ccgcacaccc tgatttcccc gaggatatca caatggtctc gtacgaagag
      781 cttttataat taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
      841 gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg
      901 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
      961 taacgaccct gccctgaacc gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg
     1021 cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag
     1081 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
     1141 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     1201 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     1261 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     1321 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     1381 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     1441 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     1501 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     1561 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     1621 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     1681 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     1741 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     1801 gtcgatactt cggcgatcac cgcttccctc atactcttcc tttttcaata ttattgaagc
     1861 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1921 caaatagcta gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg
     1981 acacatacaa agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa
     2041 acagcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca
     2101 taaacagacg cttttccggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca
     2161 gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggcggg tcgctccctc
     2221 ttgcgctctc ctgttccgac cctgccgttt accggatacc tgttccgcct ttctccctta
     2281 cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt
     2341 tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc
     2401 cggtaactgt tcacttgagt ccaacccgga aaagcacggt aaaacgccac tggcagcagc
     2461 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
     2521 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
     2581 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca
     2641 ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     2701 tcctttgatc ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt
     2761 agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc
     2821 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     2881 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2941 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3001 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     3061 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     3121 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     3181 gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg taatggcgcg
     3241 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     3301 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     3361 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     3421 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     3481 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     3541 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     3601 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     3661 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     3721 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     3781 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     3841 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     3901 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     3961 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     4021 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     4081 cgggatctcg acgctctc
//
