LOCUS       sequence-238223-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00083821
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(345..361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(345..361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(358..380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    369..385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(393..399,400..417,418..423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    438..459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(576..593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(827..846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atgggtggta aaaatggttt gaaagattgt caagtttctt tgactgctac
       61 ttctgaagct agaagatctt tttgggatac tcaatatgct gattataatg atgatgttga
      121 aatttctgct ggtagacata aatttgttat tcatgttgaa caagattgtg gtggttctaa
      181 agtttctggt actattccac cacatcattt ggttattttg tctccaatgt ctggtggtgg
      241 tagaagaact attattaatt ctgctggtga agttagattg gctgctactt gagcttagag
      301 accaagttcc atcccaatgg cgcgccgagc ttggctcgag catggtcata gctgtttcct
      361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgt
//
