LOCUS       sequence-23823-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Wang NS, Unkila MT, Reineks EZ, Distelhorst CW
  TITLE     Transient expression of wild-type or mitochondrially targeted Bcl-2 
            induces apoptosis, whereas transient expression of endoplasmic 
            reticulum-targeted Bcl-2 is protective against Bax-induced cell 
            death.
  JOURNAL   J Biol Chem. 2001 Nov 23. 276(47):44117-28.
  PUBMED    11546793
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Nov 23. 276(47):44117-28."
COMMENT     Sequence Label: Flag-Bcl2-Cb5
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..30
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     15..31
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(836..859)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      859..868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgggcccc ccctcgaggt cgacggtatc gataagcttg atgtctagat cagtcctctg
       61 ccatgtatag gcgatacatc aaggcgacgg ccactgcaga gatggcaggg atcacccagt
      121 tggtccacca actggaacta gaatcaatag tagtgatgag cagagtcttc agagacagcc
      181 aggagaaatc aaacagaggc cgcatgctgg ggccgtacag ttccacaaag gcatcccagc
      241 ctccgttatc ctggatccag gtgtgcaggt gccggttcag gtactcagtc atccacaggg
      301 cgatgttgtc caccaggggc gacatctccc ggttgacgct ctccacacac atgaccccac
      361 cgaactcaaa gaaggccaca atcctccccc agttcacccc gtccctgaag agctcctcca
      421 ccaccgtggc aaagcgtccc cgcgcggtga agggcgtcag gtgcagctgg ctggacatct
      481 cggcgaagtc gcggcggtag cggcgggaga agtcgtcgcc ggcctggcgg agggtcaggt
      541 ggaccacagg tggcaccggg ctgagcgcag gccccgaggc ggcgccgggg gcagccgggg
      601 tctgcagcgg cgaggtcctg gcgaccgggt cgcgggatgc ggctggatgg ggcgtgtgcc
      661 cgggctggga ggagaagatg cccggtgcgg gggcggcccc cgggggcgcg gcgcccacat
      721 ctcccgcatc ccactcgtag cccctctgcg acagcttata atggatgtac ttcatcacta
      781 tctcccggtt atcgtacccc gttctcccag cgtgcgccat agatccgccc gggctcttat
      841 cgtcgtcatc cttgtaatcc atggtggcgg ccgccaccgc gtgg
//
