LOCUS       sequence-238237-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00108681
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(453..469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(453..469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(466..488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    477..493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(501..507,508..525,526..531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    546..567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(684..701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atggctggtt gtattggttg tgctccatct gctccaaaag aatatgattg
       61 tgctgttgaa attagacata aagaattgga agaaccattt ttggaaattc atgctaaatt
      121 taatgatgtt attccaattg gtgttggttt ggaaactaga agaatttggt tgtggcaatg
      181 ttctgttttg tgtgatgatg ttccagctta ttggattttg aaaacttggg ctttgagaga
      241 attgccatct attactccag aatatcataa agaatgtatt gataaagctc atgaagctaa
      301 aggttctaaa tctaaaggtt ctaaatctaa aggttctaaa tctaaaggtt ctaaatctaa
      361 aggttctaaa tctaaaggtt ctaaatctaa aggtcattga gcttagagac caagttccat
      421 cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt gtgaaattgt
      481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
//
