LOCUS       sequence-238240-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00124331
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(489..505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(489..505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(502..524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    513..529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(537..543,544..561,562..567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    582..603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(720..737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atggcttctg atggtgcttc tacttctggt gttaaagcta ctggtgatat
       61 gggttctcca agaggtgttc aagatgctgg tggttctcca ggtgctccag cttcttctgt
      121 tgctcaagct tcttctggtt cttctaaacc atctagattg actagaattt ctagaattgg
      181 tagaccaaaa agatctaata gaccaaatag accaaaatct actaaaatgt gtcaaacttc
      241 tttgttgtct tctactggtg ttgttgttag aactatgtct ttggttccag gtgaagataa
      301 tttgttttct tatccagatg gtccagatca aactgaacat gttattactg ttaaaccatc
      361 taaattgact tgtaaaccat ctattactga aggtggtatt ccagaaggtt ggaaatggaa
      421 atatatgaaa gtttgagctt agagaccaag ttccatccca atggcgcgcc gagcttggct
      481 cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      781 aaggcggtaa tacggttatc cacagaatca ggggataacg ca
//
