LOCUS       sequence-238242-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00128491
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(570..586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(570..586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(583..605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(618..624,625..642,643..648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    663..684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(801..818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgatggtta ctggtgatgc ttcttcttat gttagagaac aagctagata
       61 tcatggtcaa tgtgttgttg aaatttataa ttctcaaaat caaagagctg atattttgca
      121 ttctcaaatt tggatgagag aaactttgcc agattatact ttgcatccat tgtctagata
      181 tgctgttttg gttgatggtg attgtaatgc tagagctgaa tatggtattg ctccaccagg
      241 taatactttt agaggttgta ctgcttatga atatagaact aatggtgatt cttgtccatg
      301 gccaagattg gctcaaccaa atgttgaaca taattctttt actgtttttc caggtcattt
      361 gttggctttt actactactg cttttcaagc tgaatctact actaatgctt gtagaagatc
      421 tgttgctgct ttggctactg atttggattg tattagaatg tatgttgctt tggatttggg
      481 tttgaaaaat tctttgatgg ctagattgtc tttgtgagct tagagaccaa gttccatccc
      541 aatggcgcgc cgagcttggc tcgagcatgg tcatagctgt ttcctgtgtg aaattgttat
      601 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      661 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      721 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      781 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      901 gcaggaaaga acatgtga
//
