LOCUS       sequence-238243-       15081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genome plasmid for deletion-mutant rabies virus encoding Flpo 
            recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Jin L, Matsuyama M, Sullivan HA, Zhu M, Lavin TK, Hou Y, Lea NE, 
            Pruner MT, Dam Ferdinez ML, Wickersham IR
  TITLE     ""Self-inactivating"" rabies viruses are susceptible to loss of 
            their intended attenuating modification.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2023481120. doi: 
            10.1073/pnas.2023481120. Epub 2023 Feb 6.
  PUBMED    37053554
REFERENCE   2  (bases 1 to 15081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-02-14"; volume: "120(7):e2023481120. 
            doi"; pages: " 10.1073/pnas.2023481120. Epub 2023 Feb 6"
COMMENT     Sequence Label: pRVdG-4FLPo
FEATURES             Location/Qualifiers
     source          1..15081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        656..1035
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1036..1239
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1190..1210
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1284..1303
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1284..1302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4687..4696
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4693..4698,4699..4719,4720..5991)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     CDS             7743..7754
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12753..12771)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12767..12814
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12837..12853)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12837..12853)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12890..12910)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12890..12908)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12929..12945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12929..12945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12942..12964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12953..12969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12977..12983,12984..13001,13002..13007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13022..13043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13160..13177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13331..13919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13411..13430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14090..14881,14882..14950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14713..14732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14951..15055)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgataagct tgatatcgaa ttcctgcagc gcgttgacat
      661 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      721 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      781 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      841 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      901 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      961 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1021 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     1081 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     1141 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     1201 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     1261 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
     1321 attaagcgtc tgatgagtcc gtgaggacga aacccggcgt accgggtcac gcttaacaac
     1381 cagatcaaag aaaaaacaga cattgtcaat tgcaaagcaa aaatgtaaca cccctacaat
     1441 ggatgccgac aagattgtat tcaaagtcaa taatcaggtg gtctctttga agcctgagat
     1501 tatcgtggat caatatgagt acaagtaccc tgccatcaaa gatttgaaaa agccctgtat
     1561 aaccctagga aaggctcccg atttaaataa agcatacaag tcagttttgt caggcatgag
     1621 cgccgccaaa cttaatcctg acgatgtatg ttcctatttg gcagcggcaa tgcagttttt
     1681 tgaggggaca tgtccggaag actggaccag ctatggaatt gtgattgcac gaaaaggaga
     1741 taagatcacc ccaggttctc tggtggagat aaaacgtact gatgtagaag ggaattgggc
     1801 tctgacagga ggcatggaac tgacaagaga ccccactgtc cctgagcatg cgtccttagt
     1861 cggtcttctc ttgagtctgt ataggttgag caaaatatcc gggcaaaaca ctggtaacta
     1921 taagacaaac attgcagaca ggatagagca gatttttgag acagcccctt ttgttaaaat
     1981 cgtggaacac catactctaa tgacaactca caaaatgtgt gctaattgga gtactatacc
     2041 aaacttcaga tttttggccg gaacctatga catgtttttc tcccggattg agcatctata
     2101 ttcagcaatc agagtgggca cagttgtcac tgcttatgaa gactgttcag gactggtatc
     2161 atttactggg ttcataaaac aaatcaatct caccgctaga gaggcaatac tatatttctt
     2221 ccacaagaac tttgaggaag agataagaag aatgtttgag ccagggcagg agacagctgt
     2281 tcctcactct tatttcatcc acttccgttc actaggcttg agtgggaaat ctccttattc
     2341 atcaaatgct gttggtcacg tgttcaatct cattcacttt gtaggatgct atatgggtca
     2401 agtcagatcc ctaaatgcaa cggttattgc tgcatgtgct cctcatgaaa tgtctgttct
     2461 agggggctat ctgggagagg aattcttcgg gaaagggaca tttgaaagaa gattcttcag
     2521 agatgagaaa gaacttcaag aatacgaggc ggctgaactg acaaagactg acgtagcact
     2581 ggcagatgat ggaactgtca actctgacga cgaggactac ttttcaggtg aaaccagaag
     2641 tccggaggct gtttatactc gaatcatgat gaatggaggt cgactaaaga gatctcacat
     2701 acggagatat gtctcagtca gttccaatca tcaagcccgt ccaaactcat tcgccgagtt
     2761 tctaaacaag acatattcga gtgactcata agaagttgaa taacaaaatg ccggaaatct
     2821 acggattgtg tatatccatc atgaaaaaaa ctaacacccc tcctttcgaa ccatcccaaa
     2881 catgagcaag atctttgtca atcctagtgc tattagagcc ggtctggccg atcttgagat
     2941 ggctgaagaa actgttgatc tgatcaatag aaatatcgaa gacaatcagg ctcatctcca
     3001 aggggaaccc atagaggtgg acaatctccc tgaggatatg gggcgacttc acctggatga
     3061 tggaaaatcg cccaaccatg gtgagatagc caaggtggga gaaggcaagt atcgagagga
     3121 ctttcagatg gatgaaggag aggatcctag cttcctgttc cagtcatacc tggaaaatgt
     3181 tggagtccaa atagtcagac aaatgaggtc aggagagaga tttctcaaga tatggtcaca
     3241 gaccgtagaa gagattatat cctatgtcgc ggtcaacttt cccaaccctc caggaaagtc
     3301 ttcagaggat aaatcaaccc agactactgg ccgagagctc aagaaggaga caacacccac
     3361 tccttctcag agagaaagcc aatcatcgaa agccaggatg gcggctcaaa ttgcttctgg
     3421 ccctccagcc cttgaatggt cggctaccaa tgaagaggat gatctatcag tggaggctga
     3481 gatcgctcac cagattgcag aaagtttctc caaaaaatat aagtttccct ctcgatcctc
     3541 agggatactc ttgtataatt ttgagcaatt gaaaatgaac cttgatgata tagttaaaga
     3601 ggcaaaaaat gtaccaggtg tgacccgttt agcccatgac gggtccaaac tccccctaag
     3661 atgtgtactg ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt tagtcgaatc
     3721 cgacaagctg agtaaaatca tgcaagatga cttgaatcgc tatacatctt gctaaccgaa
     3781 cctctcccct cagtccctct agacaataaa atccgagatg tcccaaagtc aacatgaaaa
     3841 aaacaggcaa caccactgat aaaatgaacc tcctacgtaa gatagtgaaa aaccgcaggg
     3901 acgaggacac tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat gacttgtggc
     3961 ttccaccccc tgaatacgtc ccgctgaaag aacttacagg caagaagaac atgaggaact
     4021 tttgtatcaa cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc aggatcctgc
     4081 ggcacattct gaaatcattc gacgagatat attctgggaa tcataggatg atcgggttag
     4141 tcaaagtggt tattggactg gctttgtcag gatctccagt ccctgagggc ctgaactggg
     4201 tatacaaatt gaggagaacc tttatcttcc agtgggctga ttccaggggc cctcttgaag
     4261 gggaggagtt ggaatactct caggagatca cttgggatga tgatactgag ttcgtcggat
     4321 tgcaaataag agtgattgca aaacagtgtc atatccaggg cagagtctgg tgtatcaaca
     4381 tgaacccgag agcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtccgaag
     4441 aggacaaaga ttcctctctg cttctagaat aatcagatta tatcccgcaa atttatcact
     4501 tgtttacctc tggaggagag aacatatggg ctcaactcca acccttggga gcaatataac
     4561 aaaaaacatg ttatggtgcc attaaaccgc tgcatttcat caaagtcaag ttgattacct
     4621 ttacattttg atcctcttgg atgtgaaaaa aactattaac atccctcaaa agaccccggg
     4681 aaagccgcca ccatggctcc taagaagaag aggaaggtga tgagccagtt cgacatcctg
     4741 tgcaagaccc cccccaaggt gctggtgcgg cagttcgtgg agagattcga gaggcccagc
     4801 ggcgagaaga tcgccagctg tgccgccgag ctgacctacc tgtgctggat gatcacccac
     4861 aacggcaccg ccatcaagag ggccaccttc atgagctaca acaccatcat cagcaacagc
     4921 ctgagcttcg acatcgtgaa caagagcctg cagttcaagt acaagaccca gaaggccacc
     4981 atcctggagg ccagcctgaa gaagctgatc cccgcctggg agttcaccat catcccttac
     5041 aacggccaga agcaccagag cgacatcacc gacatcgtgt ccagcctgca gctgcagttc
     5101 gagagcagcg aggaggccga caagggcaac agccacagca agaagatgct gaaggccctg
     5161 ctgtccgagg gcgagagcat ctgggagatc accgagaaga tcctgaacag cttcgagtac
     5221 accagcaggt tcaccaagac caagaccctg taccagttcc tgttcctggc cacattcatc
     5281 aactgcggca ggttcagcga catcaagaac gtggacccca agagcttcaa gctggtgcag
     5341 aacaagtacc tgggcgtgat cattcagtgc ctggtgaccg agaccaagac aagcgtgtcc
     5401 aggcacatct actttttcag cgccagaggc aggatcgacc ccctggtgta cctggacgag
     5461 ttcctgagga acagcgagcc cgtgctgaag agagtgaaca ggaccggcaa cagcagcagc
     5521 aacaagcagg agtaccagct gctgaaggac aacctggtgc gcagctacaa caaggccctg
     5581 aagaagaacg ccccctaccc catcttcgct atcaagaacg gccctaagag ccacatcggc
     5641 aggcacctga tgaccagctt tctgagcatg aagggcctga ccgagctgac aaacgtggtg
     5701 ggcaactgga gcgacaagag ggcctccgcc gtggccagga ccacctacac ccaccagatc
     5761 accgccatcc ccgaccacta cttcgccctg gtgtccaggt actacgccta cgaccccatc
     5821 agcaaggaga tgatcgccct gaaggacgag accaacccca tcgaggagtg gcagcacatc
     5881 gagcagctga agggcagcgc cgagggcagc atcagatacc ccgcctggaa cggcatcatc
     5941 agccaggagg tgctggacta cctgagcagc tacatcaaca ggcggatctg atgagctagc
     6001 ttataaagtg ctgggtcatc taagcttttc agtcgagaaa aaaacattag atcagaagaa
     6061 caactggcaa cacttctcaa cctgagactt acttcaagat gctcgatcct ggagaggtct
     6121 atgatgaccc tattgaccca atcgagttag aggctgaacc cagaggaacc cccattgtcc
     6181 ccaacatctt gaggaactct gactacaatc tcaactctcc tttgatagaa gatcctgcta
     6241 gactaatgtt agaatggtta aaaacaggga atagacctta tcggatgact ctaacagaca
     6301 attgctccag gtctttcaga gttttgaaag attatttcaa gaaggtagat ttgggttctc
     6361 tcaaggtggg cggaatggct gcacagtcaa tgatttctct ctggttatat ggtgcccact
     6421 ctgaatccaa caggagccgg agatgtataa cagacttggc ccatttctat tccaagtcgt
     6481 cccccataga gaagctgttg aatctcacgc taggaaatag agggctgaga atccccccag
     6541 agggagtgtt aagttgcctt gagagggttg attatgataa tgcatttgga aggtatcttg
     6601 ccaacacgta ttcctcttac ttgttcttcc atgtaatcac cttatacatg aacgccctag
     6661 actgggatga agaaaagacc atcctagcat tatggaaaga tttaacctca gtggacatcg
     6721 ggaaggactt ggtaaagttc aaagaccaaa tatggggact gctgatcgtg acaaaggact
     6781 ttgtttactc ccaaagttcc aattgtcttt ttgacagaaa ctacacactt atgctaaaag
     6841 atcttttctt gtctcgcttc aactccttaa tggtcttgct ctctccccca gagccccgat
     6901 actcagatga cttgatatct caactatgcc agctgtacat tgctggggat caagtcttgt
     6961 ctatgtgtgg aaactccggc tatgaagtca tcaaaatatt ggagccatat gtcgtgaata
     7021 gtttagtcca gagagcagaa aagtttaggc ctctcattca ttccttggga gactttcctg
     7081 tatttataaa agacaaggta agtcaacttg aagagacgtt cggtccctgt gcaagaaggt
     7141 tctttagggc tctggatcaa ttcgacaaca tacatgactt ggtttttgtg tttggctgtt
     7201 acaggcattg ggggcaccca tatatagatt atcgaaaggg tctgtcaaaa ctatatgatc
     7261 aggttcacct taaaaaaatg atagataagt cctaccagga gtgcttagca agcgacctag
     7321 ccaggaggat ccttagatgg ggttttgata agtactccaa gtggtatctg gattcaagat
     7381 tcctagcccg agaccacccc ttgactcctt atatcaaaac ccaaacatgg ccacccaaac
     7441 atattgtaga cttggtgggg gatacatggc acaagctccc gatcacgcag atctttgaga
     7501 ttcctgaatc aatggatccg tcagaaatat tggatgacaa atcacattct ttcaccagaa
     7561 cgagactagc ttcttggctg tcagaaaacc gaggggggcc tgttcctagc gaaaaagtta
     7621 ttatcacggc cctgtctaag ccgcctgtca atccccgaga gtttctgagg tctatagacc
     7681 tcggaggatt gccagatgaa gacttgataa ttggcctcaa gccaaaggaa cgggaattga
     7741 agattgaagg tcgattcttt gctctaatgt catggaatct aagattgtat tttgtcatca
     7801 ctgaaaaact cttggccaac tacatcttgc cactttttga cgcgctgact atgacagaca
     7861 acctgaacaa ggtgtttaaa aagctgatcg acagggtcac cgggcaaggg cttttggact
     7921 attcaagggt cacatatgca tttcacctgg actatgaaaa gtggaacaac catcaaagat
     7981 tagagtcaac agaggatgta ttttctgtcc tagatcaagt gtttggattg aagagagtgt
     8041 tttctagaac acacgagttt tttcaaaagg cctggatcta ttattcagac agatcagacc
     8101 tcatcgggtt acgggaggat caaatatact gcttagatgc gtccaacggc ccaacctgtt
     8161 ggaatggcca ggatggcggg ctagaaggct tacggcagaa gggctggagt ctagtcagct
     8221 tattgatgat agatagagaa tctcaaatca ggaacacaag aaccaaaata ctagctcaag
     8281 gagacaacca ggttttatgt ccgacataca tgttgtcgcc agggctatct caagaggggc
     8341 tcctctatga attggagaga atatcaagga atgcactttc gatatacaga gccgtcgagg
     8401 aaggggcatc taagctaggg ctgatcatca agaaagaaga gaccatgtgt agttatgact
     8461 tcctcatcta tggaaaaacc cctttgttta gaggtaacat attggtgcct gagtccaaaa
     8521 gatgggccag agtctcttgc gtctctaatg accaaatagt caacctcgcc aatataatgt
     8581 cgacagtgtc caccaatgcg ctaacagtgg cacaacactc tcaatctttg atcaaaccga
     8641 tgagggattt tctgctcatg tcagtacagg cagtctttca ctacctgcta tttagcccaa
     8701 tcttaaaggg aagagtttac aagattctga gcgctgaagg ggagagcttt ctcctagcca
     8761 tgtcaaggat aatctatcta gatccttctt tgggagggat atctggaatg tccctcggaa
     8821 gattccatat acgacagttc tcagaccctg tctctgaagg gttatccttc tggagagaga
     8881 tctggttaag ctcccaagag tcctggattc acgcgttgtg tcaagaggct ggaaacccag
     8941 atcttggaga gagaacactc gagagcttca ctcgccttct agaagatccg accaccttaa
     9001 atatcagagg aggggccagt cctaccattc tactcaagga tgcaatcaga aaggctttat
     9061 atgacgaggt ggacaaggtg gaaaattcag agtttcgaga ggcaatcctg ttgtccaaga
     9121 cccatagaga taattttata ctcttcttaa tatctgttga gcctctgttt cctcgatttc
     9181 tcagtgagct attcagttcg tcttttttgg gaatccccga gtcaatcatt ggattgatac
     9241 aaaactcccg aacgataaga aggcagttta gaaagagtct ctcaaaaact ttagaagaat
     9301 ccttctacaa ctcagagatc cacgggatta gtcggatgac ccagacacct cagagggttg
     9361 ggggggtgtg gccttgctct tcagagaggg cagatctact tagggagatc tcttggggaa
     9421 gaaaagtggt aggcacgaca gttcctcacc cttctgagat gttgggatta cttcccaagt
     9481 cctctatttc ttgcacttgt ggagcaacag gaggaggcaa tcctagagtt tctgtatcag
     9541 tactcccgtc ctttgatcag tcattttttt cacgaggccc cctaaaggga tacttgggct
     9601 cgtccacctc tatgtcgacc cagctattcc atgcatggga aaaagtcact aatgttcatg
     9661 tggtgaagag agctctatcg ttaaaagaat ctataaactg gttcattact agagattcca
     9721 acttggctca agctctaatt aggaacatta tgtctctgac aggccctgat ttccctctag
     9781 aggaggcccc tgtcttcaaa aggacggggt cagccttgca taggttcaag tctgccagat
     9841 acagcgaagg agggtattct tctgtctgcc cgaacctcct ctctcatatt tctgttagta
     9901 cagacaccat gtctgatttg acccaagacg ggaagaacta cgatttcatg ttccagccat
     9961 tgatgcttta tgcacagaca tggacatcag agctggtaca gagagacaca aggctaagag
    10021 actctacgtt tcattggcac ctccgatgca acaggtgtgt gagacccatt gacgacgtga
    10081 ccctggagac ctctcagatc ttcgagtttc cggatgtgtc gaaaagaata tccagaatgg
    10141 tttctggggc tgtgcctcac ttccagaggc ttcccgatat ccgtctgaga ccaggagatt
    10201 ttgaatctct aagcggtaga gaaaagtctc accatatcgg atcagctcag gggctcttat
    10261 actcaatctt agtggcaatt cacgactcag gatacaatga tggaaccatc ttccctgtca
    10321 acatatacgg caaggtttcc cctagagact atttgagagg gctcgcaagg ggagtattga
    10381 taggatcctc gatttgcttc ttgacaagaa tgacaaatat caatattaat agacctcttg
    10441 aattggtctc aggggtaatc tcatatattc tcctgaggct agataaccat ccctccttgt
    10501 acataatgct cagagaaccg tctcttagag gagagatatt ttctatccct cagaaaatcc
    10561 ccgccgctta tccaaccact atgaaagaag gcaacagatc aatcttgtgt tatctccaac
    10621 atgtgctacg ctatgagcga gagataatca cggcgtctcc agagaatgac tggctatgga
    10681 tcttttcaga ctttagaagt gccaaaatga cgtacctatc cctcattact taccagtctc
    10741 atcttctact ccagagggtt gagagaaacc tatctaagag tatgagagat aacctgcgac
    10801 aattgagttc tttgatgagg caggtgctgg gcgggcacgg agaagatacc ttagagtcag
    10861 acgacaacat tcaacgactg ctaaaagact ctttacgaag gacaagatgg gtggatcaag
    10921 aggtgcgcca tgcagctaga accatgactg gagattacag ccccaacaag aaggtgtccc
    10981 gtaaggtagg atgttcagaa tgggtctgct ctgctcaaca ggttgcagtc tctacctcag
    11041 caaacccggc ccctgtctcg gagcttgaca taagggccct ctctaagagg ttccagaacc
    11101 ctttgatctc gggcttgaga gtggttcagt gggcaaccgg tgctcattat aagcttaagc
    11161 ctattctaga tgatctcaat gttttcccat ctctctgcct tgtagttggg gacgggtcag
    11221 gggggatatc aagggcagtc ctcaacatgt ttccagatgc caagcttgtg ttcaacagtc
    11281 ttttagaggt gaatgacctg atggcttccg gaacacatcc actgcctcct tcagcaatca
    11341 tgaggggagg aaatgatatc gtctccagag tgatagatct tgactcaatc tgggaaaaac
    11401 cgtccgactt gagaaacttg gcaacctgga aatacttcca gtcagtccaa aagcaggtca
    11461 acatgtccta tgacctcatt atttgcgatg cagaagttac tgacattgca tctatcaacc
    11521 ggatcaccct gttaatgtcc gattttgcat tgtctataga tggaccactc tatttggtct
    11581 tcaaaactta tgggactatg ctagtaaatc caaactacaa ggctattcaa cacctgtcaa
    11641 gagcgttccc ctcggtcaca gggtttatca cccaagtaac ttcgtctttt tcatctgagc
    11701 tctacctccg attctccaaa cgagggaagt ttttcagaga tgctgagtac ttgacctctt
    11761 ccacccttcg agaaatgagc cttgtgttat tcaattgtag cagccccaag agtgagatgc
    11821 agagagctcg ttccttgaac tatcaggatc ttgtgagagg atttcctgaa gaaatcatat
    11881 caaatcctta caatgagatg atcataactc tgattgacag tgatgtagaa tcttttctag
    11941 tccacaagat ggttgatgat cttgagttac agaggggaac tctgtctaaa gtggctatca
    12001 ttatagccat catgatagtt ttctccaaca gagtcttcaa cgtttccaaa cccctaactg
    12061 acccctcgtt ctatccaccg tctgatccca aaatcctgag gcacttcaac atatgttgca
    12121 gtactatgat gtatctatct actgctttag gtgacgtccc tagcttcgca agacttcacg
    12181 acctgtataa cagacctata acttattact tcagaaagca agtcattcga gggaacgttt
    12241 atctatcttg gagttggtcc aacgacacct cagtgttcaa aagggtagcc tgtaattcta
    12301 gcctgagtct gtcatctcac tggatcaggt tgatttacaa gatagtgaag actaccagac
    12361 tcgttggcag catcaaggat ctatccagag aagtggaaag acaccttcat aggtacaaca
    12421 ggtggatcac cctagaggat atcagatcta gatcatccct actagactac agttgcctgt
    12481 gaaccggata ctcctggaag cctgcccatg ctaagactct tgtgtgatgt atcttgaaaa
    12541 aaacaagatc ctaaatctga acctttggtt gtttgattgt ttttctcatt tttgttgttt
    12601 atttgttaag cgtgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc
    12661 gaaggaggac gcacgtccac tcggatggct aagggagggc ggggatccgg ctgctaacaa
    12721 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
    12781 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc
    12841 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
    12901 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    12961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    13021 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    13081 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    13141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    13321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    13381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    13441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    13501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    13561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    13621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    13681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    13741 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    13801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    13861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    13921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    13981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    14041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    14101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    14161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    14221 tgataccgcg agacccaccc ttggaggctc cagatttatc agcaataaac cagccagccg
    14281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    14341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    14401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    14461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    14521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    14581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    14641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    14701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    14761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    14821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    14881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    14941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    15001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    15061 ttccccgaaa agtgccacct g
//
