LOCUS       sequence-238249-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00148071
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(443..459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(443..459)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(456..478)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    467..483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(491..497,498..515,516..521))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    536..557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(674..691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acttggtctc taagctcaag cttgagcttt agtaccagtt ttagtaccag ttttagaacc
       61 agttttagta gatggtttag tagatggttt agtaccagca gcagtatctg gtttagtaga
      121 agattttggt ggtttacctt ctttatcaac agaaccttca attttaaaac cagttggcaa
      181 agctggagaa acagaagtaa attcacattt agaattaact ttagcagtaa aaacaccagc
      241 accagaagta tcagacaaaa tagtagtacc agatggtggc aacaacatag catcaacttc
      301 tcttttatta ccattagtca aagccaattt acaagaagtt ggaactggtt ttttaaaaat
      361 atcaaaaata cctggaactg gagttggagc cattagagac caagttccat cccaatggcg
      421 cgccgagctt ggctcgagca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      601 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      901 ggtggcgaaa cccgacagga ctataaa
//
