LOCUS       sequence-238250-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00186121
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(255..271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(255..271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(268..290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    279..295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(303..309,310..327,328..333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    348..369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(486..503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(737..756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atgtctgatt gtattattag acaaattttg gctgatggta ctttgaaata
       61 tcatgaagtt aatgctaata ctagaattga agctttgaat gttatggttg ataaacattg
      121 tagaccagct gttttgtctt tgcaagatgg ttctgttttg gcttataaac attggggtag
      181 aaatactttt caaactcaat gagcttagag accaagttcc atcccaatgg cgcgccgagc
      241 ttggctcgag catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      301 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      361 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      421 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      961 cgtcttgagt ccaacccggt aaga
//
