LOCUS       sequence-238254-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_ina168_g11067
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(684..700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(684..700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(697..719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    708..724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(732..738,739..756,757..762))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    777..798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(915..932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcaacaa tatttatatc tagcacattg agcaaaaata gcataagaat
       61 ctggattttt aatagattca gtattatgaa taccatccca ttcacaacca taatctttcc
      121 attctttcaa aataatatga accatttcat gcaaaataat agtatcttga ttaccagcag
      181 taatttctct agaattaact ggattattaa aaaatggtgg acaaataaca atttcttttt
      241 cattaatcaa agcataagcc aaattatgaa cttcttctct aacacaagta taaacatttt
      301 tatcatgaca agtcaaagaa atttcatcag catctctatt acattcttta taaatttcat
      361 taaaattatt ttgaacccaa ttttgaatat cagtagaatc agttttaaaa atcaatttaa
      421 acaatctatt attagattta acagcatgat aaccccaaga agccaattca gcacaagatt
      481 tcaaagcagc tctaatttca gaagcttgag aatcagaaca ttgagaatat ctttcaatat
      541 aagctcttct tttcaaatca ccatgaatat gatttggaat tggattataa tcataaactg
      601 gatgagaaaa agtaccaata ttagtaaaag ccattagaga ccaagttcca tcccaatggc
      661 gcgccgagct tggctcgagc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      721 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      781 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      841 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      901 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      961 gtatcagctc actc
//
