LOCUS       sequence-238256-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_ina168_g2700
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(428..444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(428..444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(441..463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    452..468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(476..482,483..500,501..506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    521..542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(659..676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acttggtctc taatggctgg tgctaaatgt agagttgatt tgaaacatgg tcaagctgtt
       61 gtttcttctt ttgaagtttt ggctggttct agaaaaactg ttaaagaaga aggtttttct
      121 tggacttgtt ctactgaaag agatacttgt aatgctgtta attgtatttt gccaaaaggt
      181 ttgactgctg ttggtgttca aatttcttct ggtaaaccaa aagatagagc tgctgaagaa
      241 tctgctgcta gacaagctgc tgctccaaaa gttgttgctt tgtggttgtt ggaaaatgtt
      301 tattctggta gaaaatggcc aagaattttt aaaactggtg gtcatttggg tattttgtgg
      361 ggtgcttctg gttgagctta gagaccaagt tccatcccaa tggcgcgccg agcttggctc
      421 gagcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      481 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      541 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      601 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      661 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      721 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      781 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccat
//
