LOCUS       sequence-238257-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_ina168_g3317
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(703..719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(703..719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(716..738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    727..743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(751..757,758..775,776..781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    796..817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(934..951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttggtctct aagctcattg tctatgcaat ctttccaaca acaaatcttg agcaaaaaaa
       61 gcataagtat tagcagagtt caaagtatct ggccattgaa tatatctata tctagcatct
      121 ggatcttcaa cagcttgcaa agaatcataa ccagcataat aagtagcacc accataagaa
      181 tacaatctag aaatttctct catcaaaaca atagtttgaa acaaatcaga agtaccatcc
      241 aatttattca attccaaatt aaaccatttt ggacaaacat aaacttgatt ttcccaaaca
      301 gaagaataag ccaatggacc atctttacat ctagtttcag cttctcttgg ttctctacca
      361 gaaatataaa catcagaatt agaatcagaa caagctctac caattctatc aaaaactttc
      421 caagctctat ctctagtagt tggagaatca tctttaaaaa atctccacat ttgtttcaaa
      481 gcagtagaaa taatacctct tcttcttgga ccttcagttt catcaatcaa agctctttta
      541 gcaatttcag aagaacctct accagcaatt tcagtaatat caatttgatt atctctagcc
      601 aaaatagcac cagaagtagt tttaccatca tgattaactg gttgaaccaa cattagagac
      661 caagttccat cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt
      721 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      781 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      841 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      901 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      961 cgttcggctg cggcgagcgg tatcagctca ctcaa
//
