LOCUS       sequence-238261-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_ina168_g4175
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(334..350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(334..350)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(347..369)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    358..374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(382..388,389..406,407..412))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    427..448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(565..582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(816..835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttggtctct aatgtcttct gttgaaactg ttgctgcttc ttctgttaaa actgttccat
       61 atgtttggtg tagattggat atttatttgg ataaagatag aagatctcaa agattggcta
      121 gaaatgtttt gtttcaatct ggtgaagaag gttatgctag aggtattatt ttggaaaaac
      181 catataaatt gtatgctgat agagattgta aaactgttag attggattat gaaggtccat
      241 ctaatgttag atttattcca agaccagaaa gatctttttg agcttagaga ccaagttcca
      301 tcccaatggc gcgccgagct tggctcgagc atggtcatag ctgtttcctg tgtgaaattg
      361 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      421 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      481 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      541 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      601 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      661 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      721 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      781 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      841 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      901 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtag
//
