LOCUS       sequence-238262-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_ina168_g4590
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(636..652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(636..652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(649..671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    660..676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(684..690,691..708,709..714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    729..750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(867..884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcaataa tctttattat aagcagccaa agcaaaccaa ccaaaagtat
       61 cagcatgata caaagacaat tctttagaca aagccaaaga agccaacaaa ccataagtat
      121 tataatcttc agtatgtcta atatatggaa tatgagaagt ttcatgaacc aaaattctac
      181 ctctatcaac atcatgataa tgatatttat taaaaaaaga tggacacaaa aaaacttgat
      241 tattagattc atcagctgga tacaatggaa cataaccagc agctttatca caatgatgat
      301 ttggtggatc acatctaaaa acaatattat cagaacccaa atcacaagct ttttcaattt
      361 tatgaaaagc ttgttgcaaa gcattaatat taccttcagt atgagtttta aaatatctaa
      421 atctcaaatt agattgtttt tcaacagccc aataagcagc atcagccata gatttacacc
      481 atttagaaat caaagaaata aatttcaaac caacagcagc tttaatagta gatggagaac
      541 catcaacatc tggtttacct tgatgcaaaa ctggcaaaat agtcattaga gaccaagttc
      601 catcccaatg gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat
      661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      841 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      901 ctgcggcgag cggtatcagc tcactcaaag g
//
