LOCUS       sequence-238268-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_TH12-rn_00121641
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(521..537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(521..537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(534..556)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    545..561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(569..575,576..593,594..599))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    614..635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(752..769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcaagca tttggcattg gagctttacc tttaattcta tatctttgag
       61 attctctagt tggacccaat ggaacatacc aatctttacc agatttcatt tcaccatatg
      121 gaatttcaaa aaatttcaaa gaaccagatt gagtttgtgg atctctatta taacctttaa
      181 aagcaattgg ttgataattt ctagtcaaag taacccatgg ttcagtttta aaagttttaa
      241 ttctccaacc agctggaaaa gatctttcac cagccaaacc agcagtacaa tcttttttat
      301 tagtttgatc agcagcagta gcaaatttaa attgttcttt tttatgtgga ccttgagaaa
      361 tttcaacaac agcagtttga ccagtaacag ctttagaata accaacaaca atatcagaat
      421 tatctttaat ccaaatatga caatcagaat cattagaatc tggattattc attagagacc
      481 aagttcatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt
      541 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      601 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      721 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      781 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      841 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      901 aaaag
//
