LOCUS       sequence-238283-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_US71_00116921
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(447..463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(460..482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    471..487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(495..501,502..519,520..525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    540..561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(678..695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgttgccag ctactactca accaaataat attttggctg gtttgccaac
       61 tcaaccagct aatccagctc caaaaattca atatgatttg tgtaaagttt tgttgtggaa
      121 agataaaaaa gaacaatttg gtaataaagg ttcttcttct ggttttggtt ctccaggtaa
      181 tactgttaga gtttggatgc atccaggttt ggctgctgtt tttcaattgt ctgaagaagg
      241 ttgtgaaggt aaatttattg gtggtgaatt ggatcaaggt aaaactagaa ctccattgtc
      301 taaaggtgat ccaagattga tggaagcttt gaaagattat aaaggtgctg tttttccaga
      361 accacatggt gatgctacta tgattaaatg gtgagcttag agaccaagtt ccatcccaat
      421 ggcgcgccga gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      901 cagaggtggc gaaacccgac aggactataa agatacc
//
