LOCUS       sequence-238285-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_US71_00065681
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(411..427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(411..427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(424..446)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    435..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(459..465,466..483,484..489))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    504..525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(642..659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(893..912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atggctccat ctgatggtat ttgtactact gttcatggtg gtcaaacttt
       61 tcataatgaa ttgagatgta ttgctgaatt ttatgaacat ggtgatattt ctaattattt
      121 ttgtcatcca aaatctgatg ttaaaccagg tggtgttatt aaatgtgttt atagagatca
      181 tccaattgtt gttaaaactg gtccaggttg taattggaaa tctactgctg gtgaaatgga
      241 acaattgaaa caaaaaggtg ttgaattgaa attgttgaga agatgtatgt ctgaaagaag
      301 aaatccatat ccaccatatt cttggaatgc tggttatggt attgaacaag ctgcttgagc
      361 ttagagacca agttccatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg
      421 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      481 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      541 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      601 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgctt
//
