LOCUS       sequence-238288-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_TH16_Pwl2_100
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(441..457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(441..457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(454..476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    465..481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(489..495,496..513,514..519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    534..555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(672..689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcacata atattacaac cttcttctct ttgttctcta tgttcatgag
       61 taaattcacc tggtggacca taataaccat caccccattg accaccacca tgattaccat
      121 attcagctgg accatcacca acatgataac catgtctatt ataatattgt ctatcagatt
      181 tatcttcttg atgatctgga cccaaagaat aacctggtgg catacctcta atattaccat
      241 tattttcatg aactctaacc attctatctg gaccacctgg ataagatgga ccataattaa
      301 aatcaccttc agaacctttt ctaatagaaa tagaaccttc tctttcacct ttatcattat
      361 aaaattgttt attagtccaa ccaccaccca ttagagacca agttccatcc caatggcgcg
      421 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      481 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      541 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      601 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      661 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaag
//
