LOCUS       sequence-238380-       17094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multimeric human VWF-ECFP fusion protein with A2-Lock 
            domain The cyan fluorescence protein coding region is inserted 
            between the VWF-D'D3 and A1 domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17094)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 17094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pCS-CG-Lock-Cer-VWF
FEATURES             Location/Qualifiers
     source          1..17094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2463..2766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2767..2970
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4628..4639
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(6849..6851,6852..6854,6855..7367)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(6894..6915)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7155..7174)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7502..7523
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             12317..12340
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             12344..12364
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             12365..12382
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             12895..13075
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(13101..13118)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13107..13331
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13377..13805
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13464..13483)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13674..13695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13814..13834)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13819..14149
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14000..14135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14062..14081
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        14197..14244
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14263..14637
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    14767..14888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14804..14823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14858..14877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14937..14953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14937..14953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14950..14972)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14961..14977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14985..14991,14992..15009,15010..15015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15030..15051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15168..15185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15339..15927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15419..15438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16098..16889,16890..16958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16721..16740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16959..17063)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcccc cgggctgcag
     2401 gaattcgatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2461 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2521 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2581 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2641 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2701 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2761 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2821 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2881 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2941 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcag
     3001 gcgcgccagt cgactcccgg gatcccaatt cccgcagccc tcatttgcag gggaagatga
     3061 ttcctgccag atttgccggg gtgctgcttg ctctggccct cattttgcca gggacccttt
     3121 gtgcagaagg aactcgcggc aggtcatcca cggcccgatg cagccttttc ggaagtgact
     3181 tcgtcaacac ctttgatggg agcatgtaca gctttgcggg atactgcagt tacctcctgg
     3241 cagggggctg ccagaaacgc tccttctcga ttattgggga cttccagaat ggcaagagag
     3301 tgagcctctc cgtgtatctt ggggaatttt ttgacatcca tttgtttgtc aatggtaccg
     3361 tgacacaggg ggaccaaaga gtctccatgc cctatgcctc caaagggctg tatctagaaa
     3421 ctgaggctgg gtactacaag ctgtccggtg aggcctatgg ctttgtggcc aggatcgatg
     3481 gcagcggcaa ctttcaagtc ctgctgtcag acagatactt caacaagacc tgcgggctgt
     3541 gtggcaactt taacatcttt gctgaagatg actttatgac ccaagaaggg accttgacct
     3601 cggaccctta tgactttgcc aactcatggg ctctgagcag tggagaacag tggtgtgaac
     3661 gggcatctcc tcccagcagc tcatgcaaca tctcctctgg ggaaatgcag aagggcctgt
     3721 gggagcagtg ccagcttctg aagagcacct cggtgtttgc ccgctgccac cctctggtgg
     3781 accccgagcc ttttgtggcc ctgtgtgaga agactttgtg tgagtgtgct ggggggctgg
     3841 agtgcgcctg ccctgccctc ctggagtacg cccggacctg tgcccaggag ggaatggtgc
     3901 tgtacggctg gaccgaccac agcgcgtgca gcccagtgtg ccctgctggt atggagtata
     3961 ggcagtgtgt gtccccttgc gccaggacct gccagagcct gcacatcaat gaaatgtgtc
     4021 aggagcgatg cgtggatggc tgcagctgcc ctgagggaca gctcctggat gaaggcctct
     4081 gcgtggagag caccgagtgt ccctgcgtgc attccggaaa gcgctaccct cccggcacct
     4141 ccctctctcg agactgcaac acctgcattt gccgaaacag ccagtggatc tgcagcaatg
     4201 aagaatgtcc aggggagtgc cttgtcactg gtcaatccca cttcaagagc tttgacaaca
     4261 gatacttcac cttcagtggg atctgccagt acctgctggc ccgggattgc caggaccact
     4321 ccttctccat tgtcattgag actgtccagt gtgctgatga ccgcgacgct gtgtgcaccc
     4381 gctccgtcac cgtccggctg cctggcctgc acaacagcct tgtgaaactg aagcatgggg
     4441 caggagttgc catggatggc caggacatcc agctccccct cctgaaaggt gacctccgca
     4501 tccagcatac agtgacggcc tccgtgcgcc tcagctacgg ggaggacctg cagatggact
     4561 gggatggccg cgggaggctg ctggtgaagc tgtcccccgt ctacgccggg aagacctgcg
     4621 gcctgtgtgg gaattacaat ggcaaccagg gcgacgactt ccttaccccc tctgggctgg
     4681 cagagccccg ggtggaggac ttcgggaacg cctggaagct gcacggggac tgccaggacc
     4741 tgcagaagca gcacagcgat ccctgcgccc tcaacccgcg catgaccagg ttctccgagg
     4801 aggcgtgcgc ggtcctgacg tcccccacat tcgaggcctg ccatcgtgcc gtcagcccgc
     4861 tgccctacct gcggaactgc cgctacgacg tgtgctcctg ctcggacggc cgcgagtgcc
     4921 tgtgcggcgc cctggccagc tatgccgcgg cctgcgcggg gagaggcgtg cgcgtcgcgt
     4981 ggcgcgagcc aggccgctgt gagctgaact gcccgaaagg ccaggtgtac ctgcagtgcg
     5041 ggaccccctg caacctgacc tgccgctctc tctcttaccc ggatgaggaa tgcaatgagg
     5101 cctgcctgga gggctgcttc tgccccccag ggctctacat ggatgagagg ggggactgcg
     5161 tgcccaaggc ccagtgcccc tgttactatg acggtgagat cttccagcca gaagacatct
     5221 tctcagacca tcacaccatg tgctactgtg aggatggctt catgcactgt accatgagtg
     5281 gagtccccgg aagcttgctg cctgacgctg tcctcagcag tcccctgtct catcgcagca
     5341 aaaggagcct atcctgtcgg ccccccatgg tcaagctggt gtgtcccgct gacaacctgc
     5401 gggctgaagg gctcgagtgt accaaaacgt gccagaacta tgacctggag tgcatgagca
     5461 tgggctgtgt ctctggctgc ctctgccccc cgggcatggt ccggcatgag aacagatgtg
     5521 tggccctgga aaggtgtccc tgcttccatc agggcaagga gtatgcccct ggagaaacag
     5581 tgaagattgg ctgcaacact tgtgtctgtc gggaccggaa gtggaactgc acagaccatg
     5641 tgtgtgatgc cacgtgctcc acgatcggca tggcccacta cctcaccttc gacgggctca
     5701 aatacctgtt ccccggggag tgccagtacg ttctggtgca ggattactgc ggcagtaacc
     5761 ctgggacctt tcggatccta gtggggaata agggatgcag ccacccctca gtgaaatgca
     5821 agaaacgggt caccatcctg gtggagggag gagagattga gctgtttgac ggggaggtga
     5881 atgtgaagag gcccatgaag gatgagactc actttgaggt ggtggagtct ggccggtaca
     5941 tcattctgct gctgggcaaa gccctctccg tggtctggga ccgccacctg agcatctccg
     6001 tggtcctgaa gcagacatac caggagaaag tgtgtggcct gtgtgggaat tttgatggca
     6061 tccagaacaa tgacctcacc agcagcaacc tccaagtgga ggaagaccct gtggactttg
     6121 ggaactcctg gaaagtgagc tcgcagtgtg ctgacaccag aaaagtgcct ctggactcat
     6181 cccctgccac ctgccataac aacatcatga agcagacgat ggtggattcc tcctgtagaa
     6241 tccttaccag tgacgtcttc caggactgca acaagctggt ggaccccgag ccatatctgg
     6301 atgtctgcat ttacgacacc tgctcctgtg agtccattgg ggactgcgcc tgcttctgcg
     6361 acaccattgc tgcctatgcc cacgtgtgtg cccagcatgg caaggtggtg acctggagga
     6421 cggccacatt gtgcccccag agctgcgagg agaggaatct ccgggagaac gggtatgagt
     6481 gtgagtggcg ctataacagc tgtgcacctg cctgtcaagt cacgtgtcag caccctgagc
     6541 cactggcctg ccctgtgcag tgtgtggagg gctgccatgc ccactgccct ccagggaaaa
     6601 tcctggatga gcttttgcag acctgcgttg accctgaaga ctgtccagtg tgtgaggtgg
     6661 ctggccggcg ttttgcctca ggaaagaaag tcaccttgaa tcccagtgac cctgagcact
     6721 gccagatttg ccactgtgat gttgtcaacc tcacctgtga agcctgccag gagccgggag
     6781 gcctggtggt gcctcccaca gatgccccgg tgagccccac cactctgtat gtggaggaca
     6841 tcttcgaaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     6901 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     6961 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     7021 ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc
     7081 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     7141 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     7201 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     7261 tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc
     7321 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     7381 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     7441 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     7501 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagttcg
     7561 aaccgccatt gcacgatttc tactgcagca ggctactgga cctggtcttc ctgctggatg
     7621 gctcctccag gctgtccgag gctgagtttg aagtgctgaa ggcctttgtg gtggacatga
     7681 tggagcggct gcgcatctcc cagaagtggg tccgcgtggc cgtggtggag taccacgacg
     7741 gctcccacgc ctacatcggg ctcaaggacc ggaagcgacc gtcagagctg cggcgcattg
     7801 ccagccaggt gaagtatgcg ggcagccagg tggcctccac cagcgaggtc ttgaaataca
     7861 cactgttcca aatcttcagc aagatcgacc gccctgaagc ctcccgcatc gccctgctcc
     7921 tgatggccag ccaggagccc caacggatgt cccggaactt tgtccgctac gtccagggcc
     7981 tgaagaagaa gaaggtcatt gtgatcccgg tgggcattgg gccccatgcc aacctcaagc
     8041 agatccgcct catcgagaag caggcccctg agaacaaggc cttcgtgctg agcagtgtgg
     8101 atgagctgga gcagcaaagg gacgagatcg ttagctacct ctgtgacctt gcccctgaag
     8161 cccctcctcc tactctgccc ccccacatgg cacaagttaa cgtaggtccg gggctcttgg
     8221 gggtttcgac cctggggccc aagaggtgct ccatggttct ggatgtggcg ttcgtcctgg
     8281 aaggatcgga caaaattggt gaagccgact tcaacaggag caaggagttc atggaggagg
     8341 tgattcagcg gatggatgtg ggccaggaca gcatccacgt cacggtgctg cagtactcct
     8401 acatggtgac cgtggagtac cccttcagcg aggcacagtc caaaggggac atcctgcagc
     8461 gggtgcgaga gatccgctac cagggcggca acaggaccaa cactgggctg gccctgcggt
     8521 acctctctga ccacagcttc ttggtcagcc agggtgaccg ggagcaggcg cccaacctgg
     8581 tctacatggt caccggaaat cctgcctctg atgagatcaa gaggctgcct ggagacatcc
     8641 aggtggtgcc cattggagtg ggccctaatg ccaacgtgca ggagctggag aggattggct
     8701 ggcccaatgc ccctatcctc atccaggact ttgagacgct cccccgagag gctcctgacc
     8761 tggtgctgca gagtggttgt accggtgagg ggctgcagat ccccaccctc tcccctgcac
     8821 ctgactgcag ccagcccctg gacgtgatcc ttctcctgga tggctcctcc agtttcccag
     8881 cttcttattt tgatgaaatg aagagtttcg ccaaggcttt catttcaaaa gccaatatag
     8941 ggcctcgtct cactcaggtg tcagtgctgc agtatggaag catcaccacc attgacgtgc
     9001 catggaacgt ggtcccggag aaagcccatt tgctgagcct tgtggacgtc atgcagcggg
     9061 agggaggccc cagccaaatc ggggatgcct tgggctttgc tgtgcgatac ttgacttcag
     9121 aaatgcatgg tgccaggccg ggagcctcaa aggcggtggt catcctggtc acggacgtct
     9181 ctgtggattc agtggatgca gcagctgatg ccgccaggtc caacagagtg acagtgttcc
     9241 ctattggaat tggagatcgc tacgatgcag cccagctacg gatcttggca ggcccagcag
     9301 gcgactccaa cgtggtgaag ctccagcgaa tcgaagacct ccctaccatg gtcaccttgg
     9361 gcaattcctt cctccacaaa ctgtgctctg gatttgttag gatttgcatg gatgaggatg
     9421 ggaatgagaa gaggcccggg gacgtctgga ccttgccaga ccagtgccac accgtgactt
     9481 gccagccaga tggccagacc ttgctgaaga gtcatcgggt caactgtgac cgggggctga
     9541 ggccttcgtg ccctaacagc cagtcccctg ttaaagtgga agagacctgt ggctgccgct
     9601 ggacctgccc ctgcgtgtgc acaggcagct ccactcggca catcgtgacc tttgatgggc
     9661 agaatttcaa gctgactggc agctgttctt atgtcctatt tcaaaacaag gagcaggacc
     9721 tggaggtgat tctccataat ggtgcctgca gccctggagc aaggcagggc tgcatgaaat
     9781 ccatcgaggt gaagcacagt gccctctccg tcgagctgca cagtgacatg gaggtgacgg
     9841 tgaatgggag actggtctct gttccttacg tgggtgggaa catggaagtc aacgtttatg
     9901 gtgccatcat gcatgaggtc agattcaatc accttggtca catcttcaca ttcactccac
     9961 aaaacaatga gttccaactg cagctcagcc ccaagacttt tgcttcaaag acgtatggtc
    10021 tgtgtgggat ctgtgatgag aacggagcca atgacttcat gctgagggat ggcacagtca
    10081 ccacagactg gaaaacactt gttcaggaat ggactgtgca gcggccaggg cagacgtgcc
    10141 agcccatcct ggaggagcag tgtcttgtcc ccgacagctc ccactgccag gtcctcctct
    10201 taccactgtt tgctgaatgc cacaaggtcc tggctccagc cacattctat gccatctgcc
    10261 agcaggacag ttgccaccag gagcaagtgt gtgaggtgat cgcctcttat gcccacctct
    10321 gtcggaccaa cggggtctgc gttgactgga ggacacctga tttctgtgct atgtcatgcc
    10381 caccatctct ggtctacaac cactgtgagc atggctgtcc ccggcactgt gatggcaacg
    10441 tgagctcctg tggggaccat ccctccgaag gctgtttctg ccctccagat aaagtcatgt
    10501 tggaaggcag ctgtgtccct gaagaggcct gcactcagtg cattggtgag gatggagtcc
    10561 agcaccagtt cctggaagcc tgggtcccgg accaccagcc ctgtcagatc tgcacatgcc
    10621 tcagcgggcg gaaggtcaac tgcacaacgc agccctgccc cacggccaaa gctcccacgt
    10681 gtggcctgtg tgaagtagcc cgcctccgcc agaatgcaga ccagtgctgc cccgagtatg
    10741 agtgtgtgtg tgacccagtg agctgtgacc tgcccccagt gcctcactgt gaacgtggcc
    10801 tccagcccac actgaccaac cctggcgagt gcagacccaa cttcacctgc gcctgcagga
    10861 aggaggagtg caaaagagtg tccccaccct cctgcccccc gcaccgtttg cccacccttc
    10921 ggaagaccca gtgctgtgat gagtatgagt gtgcctgcaa ctgtgtcaac tccacagtga
    10981 gctgtcccct tgggtacttg gcctcaaccg ccaccaatga ctgtggctgt accacaacca
    11041 cctgccttcc cgacaaggtg tgtgtccacc gaagcaccat ctaccctgtg ggccagttct
    11101 gggaggaggg ctgcgatgtg tgcacctgca ccgacatgga ggatgccgtg atgggcctcc
    11161 gcgtggccca gtgctcccag aagccctgtg aggacagctg tcggtcgggc ttcacttacg
    11221 ttctgcatga aggcgagtgc tgtggaaggt gcctgccatc tgcctgtgag gtggtgactg
    11281 gctcaccgcg gggggactcc cagtcttcct ggaagagtgt cggctcccag tgggcctccc
    11341 cggagaaccc ctgcctcatc aatgagtgtg tccgagtgaa ggaggaggtc tttatacaac
    11401 aaaggaacgt ctcctgcccc cagctggagg tccctgtctg cccctcgggc tttcagctga
    11461 gctgtaagac ctcagcgtgc tgcccaagct gtcgctgtga gcgcatggag gcctgcatgc
    11521 tcaatggcac tgtcattggg cccgggaaga ctgtgatgat cgatgtgtgc acgacctgcc
    11581 gctgcatggt gcaggtgggg gtcatctctg gattcaagct ggagtgcagg aagaccacct
    11641 gcaacccctg ccccctgggt tacaaggaag aaaataacac aggtgaatgt tgtgggagat
    11701 gtttgcctac ggcttgcacc attcagctaa gaggaggaca gatcatgaca ctgaagcgtg
    11761 atgagacgct ccaggatggc tgtgatactc acttctgcaa ggtcaatgag agaggagagt
    11821 acttctggga gaagagggtc acaggctgcc caccctttga tgaacacaag tgtctggctg
    11881 agggaggtaa aattatgaaa attccaggca cctgctgtga cacatgtgag gagcctgagt
    11941 gcaacgacat cactgccagg ctgcagtatg tcaaggtggg aagctgtaag tctgaagtag
    12001 aggtggatat ccactactgc cagggcaaat gtgccagcaa agccatgtac tccattgaca
    12061 tcaacgatgt gcaggaccag tgctcctgct gctctccgac acggacggag cccatgcagg
    12121 tggccctgca ctgcaccaat ggctctgttg tgtaccatga ggttctcaat gccatggagt
    12181 gcaaatgctc ccccaggaag tgcagcaagt gagaattgct agcgccgcca ccatgattcc
    12241 tgccagattt gccggggtgc tgcttgctct ggccctcatt ttgccaggga ccctttgtac
    12301 cggtccgcgg gttaacgact acaaggacga tgacgataag ttcgaaaacc tgtacttcca
    12361 gggccatcac catcaccatc actgactcga gacctagaaa aacatggagc aatcacaagt
    12421 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
    12481 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
    12541 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
    12601 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
    12661 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
    12721 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
    12781 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
    12841 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
    12901 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    12961 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    13021 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
    13081 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    13141 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    13201 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    13261 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    13321 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
    13381 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    13441 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    13501 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc
    13561 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    13621 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    13681 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    13741 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    13801 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc
    13861 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    13921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    13981 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    14041 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
    14101 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
    14161 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
    14221 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
    14281 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
    14341 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
    14401 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
    14461 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
    14521 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
    14581 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
    14641 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
    14701 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
    14761 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    14821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    14881 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
    14941 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    15001 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    15061 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    15121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    15181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    15241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    15301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    15361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    15421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    15481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
    15541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    15601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    15661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    15721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    15781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    15841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    15901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    15961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    16021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    16081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    16141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    16201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    16261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    16321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    16381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    16441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    16501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    16561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    16621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    16681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    16741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    16801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    16861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    16921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    16981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    17041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
