LOCUS       sequence-23840-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2007 Jul . 189(13):4614-23."
COMMENT     Sequence Label: pMC1s
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(710..729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctaatgacta ttagcagctc tatgcgctgt taatcacttt acttttatct aatctagaca
       61 ttgcgaagtg attcctcctg atggtaccat gcggatcgtg ctcatttcgg gcggcgaatc
      121 tctcggcgtc gaaatgatgt atgccgtgct ggtcgtacac acgggtactc ccagagccta
      181 ctcgaccgag cacacgcagg acaaactcgc ccaccccggg tgggttctcc cgctcgtcag
      241 agacccttct cacaccgggc ctgtgcgtgc gaacgcacag atcaacgccg ggatggcgta
      301 gagctcactg cgcagcaccg tggggatgcg gccgatcatc acgtcgcgga tcgtgccgcc
      361 tcccacggcc gtgacggtgc cgacgatcca atatttctag agtgcatgca gtattaaagc
      421 gaaaaaggca tccgtcagga tggccttctg cttagctaat cagcggccgc acgcgttgcg
      481 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      541 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      601 gaaccgtaaa aaggccgctn gntcnnnctn nnnnnncncc ccctgccccc tgacgagcat
      661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttacggat
      781 acctgtccgc cttctccctc ggaag
//
