LOCUS       sequence-238416-        2025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of MARIO, a genetically-encoded fluorescent 
            Mg2+ sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, 
            Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T
  TITLE     A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis
            Contributes to Mitotic Chromosome Condensation.
  JOURNAL   Curr Biol. 2018 Feb 5;28(3):444-451.e6. doi: 
            10.1016/j.cub.2017.12.035. Epub 2018 Jan 18.
  PUBMED    29358072
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2017.12.035"; journalName: "Curr Biol"; date: 
            "2018-02-5- 5"; volume: "28"; issue: "3"; pages: "444-451.e6"
COMMENT     Sequence Label: MARIO/ pcDNA3
FEATURES             Location/Qualifiers
     source          1..2025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..519)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             1288..2022
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTAVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgcccgcatg ctgagcctgg caacccgccc ggaactggaa
      721 gacatcgaag catcggcacg tttctttgaa gacgacgacg gcctgcatga tcactccttc
      781 ttcttctttg aagatgcgga agatcacgcc ggtaactcca ctgtggcatt taccatccgt
      841 gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg cttttcgtct gtatcgtatg
      901 cgtgcccgta gccagtcgat ggtagacggt aacgcctacg agttgctgct ggatctgttc
      961 gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata tctatagcga cctggagcag
     1021 ttgagccggg tgattatgga agggcatcag ggcgatgagt acgacgaggc gctctccact
     1081 ctggcggaac tggaagatga cggctgggcc gttgccctgt gtctgatgga tacccagcgc
     1141 gcgctcaact tcctggtgcg taaagcgcgt ttaccgggtg ggcaactgga gcaggcgcgt
     1201 gaaatcctgc gagatatcga atccctgctg ccgcataacg aatccctgtt ccagaaggtg
     1261 aacttcctga tgcaggcggc tgagctcatg gacggcggcg tgcagctcgc cgaccactac
     1321 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1381 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1441 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg tggcagcggt
     1501 ggcatggtga gcaagggcga ggagctgttc accgcggtgg tgcccatcct ggtcgagctg
     1561 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1621 tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc
     1681 accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     1741 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1801 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1861 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1921 cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag
     1981 aacggcatca aggccaactt caagatccgc cacaacatcg agtaa
//
