LOCUS       sequence-238418-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Gamillus, an acid-tolerant monomeric green 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T
  TITLE     Acid-Tolerant Monomeric GFP from Olindias formosa.
  JOURNAL   Cell Chem Biol. 2018 Mar 15;25(3):330-338.e7. doi: 
            10.1016/j.chembiol.2017.12.005. Epub 2017 Dec 28.
  PUBMED    29290624
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2017.12.005"; journalName: "Cell Chem Biol"; 
            date: "2018-03-15- 15"; volume: "25"; issue: "3"; pages: 
            "330-338.e7"
COMMENT     Sequence Label: Gamillus/ pcDNA3
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..717
                     /codon_start=1
                     /product="acid-tolerant monomeric green fluorescent protein
                     (Shinoda et al., 2018)"
                     /label=Gamillus
                     /translation="MVSKGEEASGRALFQYPMTSKIELNGEINGKKFKVAGEGFTPSSG
                     RFNMHAYCTTGDLPMSWVVIASPLQYGFHMFAHYPEDITHFFQECFPGSYTLDRTLRME
                     GDGTLTTHHEYSLEDGCVTSKTTLNASGFDPKGATMTKSFVKQLPNEVKITPHGPNGIR
                     LTSTVLYLKEDGTIQIGTQDCIVTPVGGRKVTQPKAHFLHTQIIQKKDPNDTRDHIVQT
                     ELAVAGNLWHGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga ggcatctggc agagccctgt tccagtaccc catgaccagc
       61 aagatcgagc tgaacggcga gatcaacggc aagaaattca aggtggccgg cgagggcttc
      121 acccccagca gcggcagatt caacatgcac gcctactgca ccaccggcga cctgcctatg
      181 agctgggtcg tgattgccag ccccctccag tacggcttcc acatgttcgc ccactacccc
      241 gaggacatca cacacttttt ccaggaatgc ttccccggca gctacaccct ggaccggacc
      301 ctgagaatgg aaggcgacgg caccctgacc acccaccacg agtacagcct ggaggacggc
      361 tgcgtgacct ccaagaccac cctgaatgcc agcggcttcg accctaaggg cgccaccatg
      421 accaagagct tcgtgaaaca actgcctaac gaggtgaaga tcacccccca cggccccaac
      481 ggcatcagac tgaccagcac cgtgctgtac ctgaaggagg atggcaccat ccagatcggc
      541 acccaggact gcatcgtgac ccctgtgggc ggaaggaaag tgacccagcc caaggcccac
      601 ttcctgcaca cccagatcat ccagaagaag gaccccaacg acacccggga ccacatcgtg
      661 cagacagaac tggccgtggc cggcaatctg tggcacggca tggacgagct gtacaag
//
