LOCUS       sequence-238427-        3531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ChIC; genomic mapping of chromatin proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Schmid M, Durussel T, Laemmli UK
  TITLE     ChIC and ChEC; genomic mapping of chromatin proteins.
  JOURNAL   Mol Cell. 2004 Oct 8;16(1):147-57.
  PUBMED    15469830
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-10-8"; volume: "16(1)"; pages: "147-57"
COMMENT     Sequence Label: pK19pA-MN
FEATURES             Location/Qualifiers
     source          1..3531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    42..63
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(78..83,84..101,102..108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    116..132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     121..143
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     140..156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     140..156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             170..595
                     /codon_start=1
                     /product="immunoglobulin binding domains of Staphylocuccus 
                     aureus protein A"
                     /label=protein A
                     /translation="SLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHL
                     PNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLP
                     NLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPK"
     CDS             614..1060
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ"
     primer_bind     complement(1098..1115)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1098..1114)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1107..1129)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1619..2413
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1673..1692)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2283..2302
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      2697..3285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3186..3205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3439..3456
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
       61 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      121 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttaaaaga
      181 tgacccaagc caaagtgcta acctattgtc agaagctaaa aagttaaatg aatctcaagc
      241 accgaaagcg gataacaaat tcaacaaaga acaacaaaat gctttctatg aaatcttaca
      301 tttacctaac ttaaacgaag aacaacgcaa tggtttcatc caaagcctaa aagatgaccc
      361 aagccaaagc gctaaccttt tagcagaagc taaaaagcta aatgatgctc aagcaccaaa
      421 agctgacaac aaattcaaca aagaacaaca aaatgctttc tatgaaattt tacatttacc
      481 taacttaact gaagaacaac gtaacggctt catccaaagc cttaaagacg atccttcagt
      541 gagcaaagaa attttagcag aagctaaaaa gctaaacgat gctcaagcac caaaagatga
      601 cgataaagaa ttcgcaactt caactaaaaa attacataaa gaacctgcga ctttaattaa
      661 agcgattgat ggtgatacgg ttaaattaat gtacaaaggt caaccaatga cattcagact
      721 attattggtc gacacacctg aaacaaagca tcctaaaaaa ggtgtagaga aatatggtcc
      781 tgaagcaagt gcatttacca aaaaaatggt agaaaatgca aagaaaattg aagtcgaatt
      841 cgacaaaggt caaagaactg ataaatatgg acgtgggcta gcgtatattt atgctgatgg
      901 aaaaatggta aacgaagctt tagttcgtca aggcttggct aaagttgctt atgtttacaa
      961 acctaacaat acacatgaac aacatttaag aaaaagtgaa gcacaagcga aaaaagagaa
     1021 attaaatatt tggagcgaag acaacgctga ttcaggtcaa taatgaggat ccccgggtac
     1081 cgagctcgaa ttaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1141 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1201 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgataa
     1261 gctagcttca cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac
     1321 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
     1381 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
     1441 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
     1501 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     1561 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
     1621 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     1681 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     1741 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
     1801 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1861 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1921 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     1981 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     2041 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     2101 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg
     2161 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     2221 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     2281 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     2341 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     2401 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     2461 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     2521 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     2581 caccccaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2641 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2701 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2761 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2821 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2881 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2941 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3001 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3061 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3121 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3181 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3241 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3301 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3361 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3421 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3481 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga c
//
