LOCUS       sequence-238435-        6970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for ChrimsonR-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pCAG-ChrimsonR-tdT
FEATURES             Location/Qualifiers
     source          1..6970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     782..804
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     866..885
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      919..928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             925..1974
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=ChrimsonR
                     /note="K176R mutant with accelerated closing kinetics"
                     /translation="MAELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTE
                     WWCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSI
                     AIALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRY
                     FEWLLSCPVILIRLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIV
                     SCIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLK
                     LSPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEE
                     FVEEEDEDTV"
     primer_bind     complement(3507..3526)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3572..3627
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3626..3645)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3988..4004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3988..4004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4001..4023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4012..4028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4036..4042,4043..4060,4061..4066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4081..4102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4161..4356
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4207..4342
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4362..4496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4399..4418)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4453..4472
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4564..4581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4735..5323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4815..4834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5494..6285,6286..6354))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6117..6136
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6355..6459)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6489..6868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
       61 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
      121 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
      181 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
      241 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
      301 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
      361 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
      421 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc
      481 ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
      541 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa
      601 tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc
      661 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct
      721 ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
      781 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
      841 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
      901 ggcaaagaat tctgagccgc caccatggct gagctgatca gcagcgccac cagatctctg
      961 tttgccgccg gaggcatcaa cccttggcct aacccctacc accacgagga catgggctgt
     1021 ggaggaatga cacctacagg cgagtgcttc agcaccgagt ggtggtgtga cccttcttac
     1081 ggactgagcg acgccggata cggatattgc ttcgtggagg ccacaggcgg ctacctggtc
     1141 gtgggagtgg agaagaagca ggcttggctg cacagcagag gcacaccagg agaaaagatc
     1201 ggcgcccagg tctgccagtg gattgctttc agcatcgcca tcgccctgct gacattctac
     1261 ggcttcagcg cctggaaggc cacttgcggt tgggaggagg tctacgtctg ttgcgtcgag
     1321 gtgctgttcg tgaccctgga gatcttcaag gagttcagca gccccgccac agtgtacctg
     1381 tctaccggca accacgccta ttgcctgcgc tacttcgagt ggctgctgtc ttgccccgtg
     1441 atcctgatca gactgagcaa cctgagcggc ctgaagaacg actacagcaa gcggaccatg
     1501 ggcctgatcg tgtcttgcgt gggaatgatc gtgttcggca tggccgcagg actggctacc
     1561 gattggctca agtggctgct gtatatcgtg tcttgcatct acggcggcta catgtacttc
     1621 caggccgcca agtgctacgt ggaagccaac cacagcgtgc ctaaaggcca ttgccgcatg
     1681 gtcgtgaagc tgatggccta cgcttacttc gcctcttggg gcagctaccc aatcctctgg
     1741 gcagtgggac cagaaggact gctgaagctg agcccttacg ccaacagcat cggccacagc
     1801 atctgcgaca tcatcgccaa ggagttttgg accttcctgg cccaccacct gaggatcaag
     1861 atccacgagc acatcctgat ccacggcgac atccggaaga ccaccaagat ggagatcgga
     1921 ggcgaggagg tggaagtgga agagttcgtg gaggaggagg acgaggacac agtggcggca
     1981 ccggtagtag cagtgagtaa gggcgaggaa gtgatcaaag agttcatgcg gtttaaggtg
     2041 agaatggaag gaagcatgaa cggccacgag ttcgaaattg agggagaagg agagggacgg
     2101 ccctacgagg gcacccagac agccaagctg aaagtgacaa agggcgggcc tctgccattc
     2161 gcttgggaca tcctgagccc acagtttatg tacggctcca aggcctatgt gaaacatcca
     2221 gctgacattc ccgattataa gaaactgagc ttccccgagg ggtttaagtg ggaaagagtg
     2281 atgaacttcg aggacggagg cctggtgact gtgacccagg acagctccct gcaggatggg
     2341 accctgatct acaaggtgaa aatgagaggg acaaattttc cccctgatgg acctgtgatg
     2401 cagaagaaaa ctatgggatg ggaggcctcc accgaaaggc tgtatccacg cgacggggtg
     2461 ctgaaaggag aaatccacca ggctctgaag ctgaaagatg ggggacatta cctggtggag
     2521 ttcaagacaa tctacatggc caagaaacct gtgcagctgc caggctacta ttacgtggac
     2581 acaaaactgg atatcacttc acacaacgag gactacacta ttgtggagca gtatgaacgg
     2641 agcgagggga gacaccatct gttcctgggc catgggactg gaagtaccgg ctcagggtct
     2701 agtggaaccg cctcaagcga ggataacaat atggctgtga tcaaagagtt catgaggttt
     2761 aaggtgcgca tggagggcag catgaatggg cacgaatttg agattgaagg agagggcgaa
     2821 gggaggcctt acgagggcac acagactgcc aagctgaaag tgaccaaggg aggaccactg
     2881 cctttcgctt gggatatcct gtctcctcag tttatgtacg gaagtaaggc ctatgtcaag
     2941 catcccgctg acattcctga ttacaagaaa ctgtctttcc cagagggctt taagtgggag
     3001 agagtgatga attttgaaga tggaggcctg gtgaccgtga cacaggactc ctctctgcag
     3061 gatggcactc tgatctacaa agtcaaaatg cgcggcacca attttccacc cgatgggccc
     3121 gtgatgcaga agaaaacaat ggggtgggag gccagcactg aacggctgta tcctagagac
     3181 ggagtgctga agggcgaaat ccaccaggcc ctgaagctga aagacggcgg ccactacctg
     3241 gtggagttca aaaccatcta catggccaag aaaccagtgc agctgcccgg ctattactat
     3301 gtggacacca agctggatat cacatcccac aatgaagact acaccattgt ggaacagtat
     3361 gagaggtctg aaggacgcca ccatctgttt ctgtacggca tggatgagct gtataagtaa
     3421 agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     3481 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     3541 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3601 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     3661 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     3721 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     3781 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     3841 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     3901 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     3961 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4141 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4201 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4261 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4321 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     4381 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4441 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     4501 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4561 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4621 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4681 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4741 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4801 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4861 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4921 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5161 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5341 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5401 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5461 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5521 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5581 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5641 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5701 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5761 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5821 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5881 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5941 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6001 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6061 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6121 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6181 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6241 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6301 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6361 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6421 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6481 acctggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     6541 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     6601 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     6661 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     6721 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     6781 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     6841 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     6901 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     6961 tgcagcgatg
//
