LOCUS       sequence-238437-       10801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 10801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKlux2
FEATURES             Location/Qualifiers
     source          1..10801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(265..270,271..288,289..295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     362..382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        364..382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     411..427
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     412..428
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             466..1908
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1921..2844
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2893..3975
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3990..4973
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5152..6264
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     complement(6309..6325)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6309..6325)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6357..6376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6358..6376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6386..6403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6386..6402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6395..6417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(7111..7773)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7774..8545
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(join(9870..10661,10662..10730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10493..10512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10731..10801,1))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
       61 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtttttatg
      121 catgcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaggtcga
      421 cggtatcgat aagcttgata tcgaattcag gcttggagga tacgtatgac taaaaaaatt
      481 tcattcatta ttaacggcca ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc
      541 attaattttg gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac
      601 attattgatt gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat
      661 acggtagggc aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac
      721 ttaaaaaaat atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct
      781 atgattttat gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc
      841 catatcatgg atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt
      901 aaatctgtac atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc
      961 gcaattttaa ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct
     1021 aatgcattag cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta
     1081 tctgttatat attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcaacat
     1141 gcggatgtta ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcatgcg
     1201 ccatcttatg ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat
     1261 cctgttgatt tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag
     1321 cgagcttgtt tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag
     1381 ttagcgttga tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat
     1441 tttgatgaaa aggcggccta ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa
     1501 gtagaggtgg atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt
     1561 aatcaaccac ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg
     1621 ccttatgttc aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt
     1681 aaatatcgag atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat
     1741 aacatatttc gagttggtgg atctcatgac ggaatgagac cgttgcaacg attagtgaca
     1801 tatatttctc atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa
     1861 cagactcgat tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaaagt
     1921 atggaaaatg aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa
     1981 aaaattcatg tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt
     2041 attattgcgt ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta
     2101 tcgcggaatg gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca
     2161 gggacaattg atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat
     2221 tggttaacta cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg
     2281 atagcttatg caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt
     2341 gttaacttaa gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt
     2401 aatgaattgc cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg
     2461 agagattgtc ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat
     2521 cttgatatac cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa
     2581 gttatcacat tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga
     2641 agttcgcatg acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg
     2701 aaagccgcta tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg
     2761 tcatttgaac atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa
     2821 aatcaagcaa tttctctgtc ttaaaatcta ttgagatatt ctatcactca aatagcaata
     2881 taaggactct ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct
     2941 caaacagagg taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt
     3001 gataccgtat ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat
     3061 gtcgctgctg catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt
     3121 gttcttccca cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg
     3181 tcaaaaggac gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta
     3241 ttcggcacag atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag
     3301 aatggcatga cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta
     3361 aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca
     3421 gcttcgacga ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata
     3481 aatactaacg aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg
     3541 cacgatattc ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca
     3601 attaaagcga aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat
     3661 gctacgacta tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag
     3721 tggcgtgact ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac
     3781 gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat
     3841 gctacaggaa tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt
     3901 attgcttcca tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt
     3961 tcgctattat attagctaag gagaaagaaa tgaaatttgg attgttcttc cttaacttca
     4021 tcaattcaac aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg
     4081 ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg
     4141 ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa
     4201 ttggttcatt aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt
     4261 gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa
     4321 aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt
     4381 gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat aacgattttt
     4441 atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat
     4501 atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca
     4561 tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg
     4621 ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta
     4681 actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg
     4741 ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa
     4801 acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg
     4861 gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg
     4921 taatcaatat tgttgatgat aatattaaga agtaccacat ggaatatacc taatagattt
     4981 cgagttgcag cgaggcggca agtgaacgaa tccccaggag catagataac tatgtgactg
     5041 gggtgagtga aagcagccaa caaagcagca gcttgaaaga tgaagggtat aaaagagtat
     5101 gacagcagtg ctgccatact ttctaatatt atcttgagga gtaaaacagg tatgacttca
     5161 tatgttgata aacaagaaat tacagcaagc tcagaaattg atgatttgat tttttcgagc
     5221 gatccattag tgtggtctta cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat
     5281 gcatttcgta atcattataa acattgtcga gaatatcgtc actactgtca ggcacacaaa
     5341 gtagatgaca atattacgga aattgatgac atacctgtat tcccaacatc ggtttttaag
     5401 tttactcgct tattaacttc tcaggaaaac gagattgaaa gttggtttac cagtagcggc
     5461 acgaatggtt taaaaagtca ggtggcgcgt gacagattaa gtattgagag actcttaggc
     5521 tctgtgagtt atggcatgaa atatgttggt agttggtttg atcatcaaat agaattagtc
     5581 aatttgggac cagatagatt taatgctcat aatatttggt ttaaatatgt tatgagtttg
     5641 gtggaattgt tatatcctac gacatttacc gtaacagaag aacgaataga ttttgttaaa
     5701 acattgaata gtcttgaacg aataaaaaat caagggaaag atctttgtct tattggttcg
     5761 ccatacttta tttatttact ctgccattat atgaaagata aaaaaatctc attttctgga
     5821 gataaaagcc tttatatcat aaccggaggc ggctggaaaa gttacgaaaa agaatctctg
     5881 aaacgtgatg atttcaatca tcttttattt gatactttca atctcagtga tattagtcag
     5941 atccgagata tatttaatca agttgaactc aacacttgtt tctttgagga tgaaatgcag
     6001 cgtaaacatg ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct
     6061 gtacctgatg gaacgccggg gttgatgagt tatatggatg cgtcagcaac cagttatcca
     6121 gcatttattg ttaccgatga tgtcgggata attagcagag aatatggtaa gtatcccggc
     6181 gtgctcgttg aaattttacg tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc
     6241 ttaaccgaag cgtttgatag ttgatgacct gcaggtcgac ggatccgggg aattcctgca
     6301 gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc aattcgccct
     6361 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6541 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6601 attttttaac caataggccg tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     6661 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
     6721 tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc agggtcgtta
     6781 aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa gcagtgtgac
     6841 cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat
     6901 tgacgatatg atcatttatt ctgcctccca gagcctgata aaaacggtga atccgttagc
     6961 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     7021 gcggggaggc agacaaggta tagggcggcg aggcggctac agccgatagt ctggaacagc
     7081 gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc ggcagcgtga cccgtgtcgg
     7141 cggctccaac ggctcgccat cgtccagaaa acacggctca tcgggcatcg gcaggcgctg
     7201 ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc
     7261 cggaatgccg ggctggctgg gcggctcctc gccggggccg gtcggtagtt gctgctcgcc
     7321 cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc
     7381 gtcgtgatca accaccacgg cggcactgaa caccgacagg cgcaactggt cgcggggctg
     7441 gccccacgcc acgcggtcat tgaccacgta ggccgacacg gtgccggggc cgttgagctt
     7501 cacgacggag atccagcgct cggccaccaa gtccttgact gcgtattgga ccgtccgcaa
     7561 agaacgtccg atgagcttgg aaagtgtctt ctggctgacc accacggcgt tctggtggcc
     7621 catctgcgcc acgaggtgat gcagcagcat tgccgccgtg ggtttcctcg caataagccc
     7681 ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag tgaccgggct tgttcttggc
     7741 ttgaatgccg atttctctgg actgcgtggc catgcttatc tccatgcggt aggggtgccg
     7801 cacggttgcg gcaccatgcg caatcagctg caacttttcg gcagcgcgac aacaattatg
     7861 cgttgcgtaa aagtggcagt caattacaga ttttctttaa cctacgcaat gagctattgc
     7921 ggggggtgcc gcaatgagct gttgcgtacc cccctttttt aagttgttga tttttaagtc
     7981 tttcgcattt cgccctatat ctagttcttt ggtgcccaaa gaagggcacc cctgcggggt
     8041 tcccccacgc cttcggcgcg gctccccctc cggcaaaaag tggcccctcc ggggcttgtt
     8101 gatcgactgc gcggccttcg gccttgccca aggtggcgct gcccccttgg aacccccgca
     8161 ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc gcccttcgac tgcccccact
     8221 cgcataggct tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg
     8281 agccttgacc cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc
     8341 cggaggcttt tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca
     8401 gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca
     8461 agctcgtggg caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga
     8521 gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa
     8581 gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc
     8641 gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg
     8701 gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag
     8761 gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg
     8821 tggcggccag tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca
     8881 ggacgccaga gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc
     8941 gcgagttgct gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga
     9001 cggccagccc ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga
     9061 aggcgcacaa gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc
     9121 accgtgacga aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca
     9181 ggctgtcggc caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt
     9241 ttgcggccgc tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc
     9301 acacgcgcat tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca
     9361 tcagcccgca agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg
     9421 gaatctcaaa gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc
     9481 ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg
     9541 ccgatcaagc ccaagagacg gcccgagacc ttcgggagcg cctgaagccc gttctggacg
     9601 ccctggggcc gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg
     9661 gcgaaaagct gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc
     9721 aggaacgcgg gcgcgcacat ttccccgaaa agtgccacct ggattttggt catgagatta
     9781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10801 t
//
