LOCUS       sequence-238476-         306 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutagenesis of Grin2b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Hunker AC, Soden ME, Krayushkina D, Heymann G, Awatramani R, Zweifel
            LS
  TITLE     Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient 
            Mutagenesis in the Adult Mouse Nervous System.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4303-4316.e6. doi: 
            10.1016/j.celrep.2020.02.092.
  PUBMED    32209486
REFERENCE   2  (bases 1 to 306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4303-4316.e6"
COMMENT     Sequence Label: pAAV-FLEXfrt-SaCas9-U6-sgGrin2b
FEATURES             Location/Qualifiers
     source          1..306
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..150
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        231..306
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
       61 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      121 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      181 atatatcttg tggaaaggac gaaacaccgc acttcgtcgg aagtgcccac gttttagtac
      241 tctggaaaca gaatctacta aaacaaggca aaatgccgtg tttatctcgt caacttgttg
      301 gcgaga
//
