LOCUS       sequence-238479-         240 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutagenesis of Gria2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Hunker AC, Soden ME, Krayushkina D, Heymann G, Awatramani R, Zweifel
            LS
  TITLE     Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient 
            Mutagenesis in the Adult Mouse Nervous System.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4303-4316.e6. doi: 
            10.1016/j.celrep.2020.02.092.
  PUBMED    32209486
REFERENCE   2  (bases 1 to 240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4303-4316.e6"
COMMENT     Sequence Label: pAAV-FLEXfrt-SaCas9-U6-sgGria2
FEATURES             Location/Qualifiers
     source          1..240
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        165..240
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
       61 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      121 cttgtggaaa ggacgaaaca ccgtatttcc aaggggcgct gatcgtttta gtactctgga
      181 aacagaatct actaaaacaa ggcaaaatgc cgtgtttatc tcgtcaactt gttggcgaga
//
