LOCUS       sequence-238485-        6079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Kang S, Denley A, Vanhaesebroeck B, Vogt PK
  TITLE     Oncogenic transformation induced by the p110beta, -gamma, and -delta
            isoforms of class I phosphoinositide 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1289-94. Epub 2006 Jan 
            23.
  PUBMED    16432180
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2006-01-31"; volume: "103(5)"; pages: 
            "1289-94. Epub 2006 Jan 23"
COMMENT     Sequence Label: pBSFI-p110-delta
FEATURES             Location/Qualifiers
     source          1..6079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    87..108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(123..128,129..146,147..153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     185..201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     185..201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     220..240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        222..240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3485..3504)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3486..3504)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3514..3531)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3514..3530)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3523..3545)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3671..4127)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3809..3830)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4021..4040
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4154..4258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4259..4327,4328..5119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4477..4496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5290..5878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5779..5798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6032..6049
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
       61 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      241 gaacaaaagc tggagctggc cattacggcc gcggccgcct ctagaggatc caccatggcc
      301 cctggggtgg actgccccat ggaattctgg accaaggagg agaatcagag cgttgtggtt
      361 gacttcctgc tgcccacagg ggtctacctg aacttccctg tgtcccgcaa tgccaacctc
      421 agcaccatca agcagctgct gtggcaccgc gcccagtatg agccgctctt ccacatgctc
      481 agtggccccg aggcctatgt gttcacctgc atcaaccaga cagcggagca gcaagagctg
      541 gaggacgagc aacggcgtct gtgtgacgtg cagcccttcc tgcccgtcct gcgcctggtg
      601 gcccgtgagg gcgaccgcgt gaagaagctc atcaactcac agatcagcct cctcatcggc
      661 aaaggcctcc acgagtttga ctccttgtgc gacccagaag tgaacgactt tcgcgccaag
      721 atgtgccaat tctgcgagga ggcggccgcc cgccggcagc agctgggctg ggaggcctgg
      781 ctgcagtaca gtttccccct gcagctggag ccctcggctc aaacctgggg gcctggtacc
      841 ctgcggctcc cgaaccgggc ccttctggtc aacgttaagt ttgagggcag cgaggagagc
      901 ttcaccttcc aggtgtccac caaggacgtg ccgctggcgc tgatggcctg tgccctgcgg
      961 aagaaggcca cagtgttccg gcagccgctg gtggagcagc cggaagacta cacgctgcag
     1021 gtgaacggca ggcatgagta cctgtatggc agctacccgc tctgccagtt ccagtacatc
     1081 tgcagctgcc tgcacagtgg gttgacccct cacctgacca tggtccattc ctcctccatc
     1141 ctcgccatgc gggatgagca gagcaaccct gccccccagg tccagaaacc gcgtgccaaa
     1201 ccacctccca ttcctgcgaa gaagccttcc tctgtgtccc tgtggtccct ggagcagccg
     1261 ttccgcatcg agctcatcca gggcagcaaa gtgaacgccg acgagcggat gaagctggtg
     1321 gtgcaggccg ggcttttcca cggcaacgag atgctgtgca agacggtgtc cagctcggag
     1381 gtgagcgtgt gctcggagcc cgtgtggaag cagcggctgg agttcgacat caacatctgc
     1441 gacctgcccc gcatggcccg tctctgcttt gcgctgtacg ccgtgatcga gaaagccaag
     1501 aaggctcgct ccaccaagaa gaagtccaag aaggcggact gccccattgc ctgggccaac
     1561 ctcatgctgt ttgactacaa ggaccagctt aagaccgggg aacgctgcct ctacatgtgg
     1621 ccctccgtcc cagatgagaa gggcgagctg ctgaacccca cgggcactgt gcgcagtaac
     1681 cccaacacgg atagcgccgc tgccctgctc atctgcctgc ccgaggtggc cccgcacccc
     1741 gtgtactacc ccgccctgga gaagatcttg gagctggggc gacacagcga gtgtgtgcat
     1801 gtcaccgagg aggagcagct gcagctgcgg gaaatcctgg agcggcgggg gtctggggag
     1861 ctgtatgagc acgagaagga cctggtgtgg aagctgcggc atgaagtcca ggagcacttc
     1921 ccggaggcgc tagcccggct gctgctggtc accaagtgga acaagcatga ggatgtggcc
     1981 cagatgctct acctgctgtg ctcctggccg gagctgcccg tcctgagcgc cctggagctg
     2041 ctagacttca gcttccccga ttgccacgta ggctccttcg ccatcaagtc gctgcggaaa
     2101 ctgacggacg atgagctgtt ccagtacctg ctgcagctgg tgcaggtgct caagtacgag
     2161 tcctacctgg actgcgagct gaccaaattc ctgctggacc gggccctggc caaccgcaag
     2221 atcggccact tccttttctg gcacctccgc tccgagatgc acgtgccgtc ggtggccctg
     2281 cgcttcggcc tcatcctgga ggcctactgc aggggcagca cccaccacat gaaggtgctg
     2341 atgaagcagg gggaagcact gagcaaactg aaggccctga atgacttcgt caagctgagc
     2401 tctcagaaga cccccaagcc ccagaccaag gagctgatgc acttgtgcat gcggcaggag
     2461 gcctacctag aggccctctc ccacctgcag tccccactcg accccagcac cctgctggct
     2521 gaagtctgcg tggagcagtg caccttcatg gactccaaga tgaagcccct gtggatcatg
     2581 tacagcaacg aggaggcagg cagcggcggc agcgtgggca tcatctttaa gaacggggat
     2641 gacctccggc aggacatgct gaccctgcag atgatccagc tcatggacgt cctgtggaag
     2701 caggaggggc tggacctgag gatgaccccc tatggctgcc tccccaccgg ggaccgcaca
     2761 ggcctcattg aggtggtact ccgttcagac accatcgcca acatccaact caacaagagc
     2821 aacatggcag ccacagccgc cttcaacaag gatgccctgc tcaactggct gaagtccaag
     2881 aacccggggg aggccctgga tcgagccatt gaggagttca ccctctcctg tgctggctat
     2941 tgtgtggcca catatgtgct gggcattggc gatcggcaca gcgacaacat catgatccga
     3001 gagagtgggc agctgttcca cattgatttt ggccactttc tggggaattt caagaccaag
     3061 tttggaatca accgcgagcg tgtcccattc atcctcacct acgactttgt ccatgtgatt
     3121 cagcagggga agactaataa tagtgagaaa tttgaacggt tccggggcta ctgtgaaagg
     3181 gcctacacca tcctgcggcg ccacgggctt ctcttcctcc acctctttgc cctgatgcgg
     3241 gcggcaggcc tgcctgagct cagctgctcc aaagacatcc agtatctcaa ggactccctg
     3301 gcactgggga aaacagagga ggaggcactg aagcacttcc gagtgaagtt taacgaagcc
     3361 ctccgtgaga gctggaaaac caaagtgaac tggctggccc acaacgtgtc caaagacaac
     3421 aggcagtaga cgcgtaagct tatcgatgtc gacgggcccg gccgcctcgg ccgtacccaa
     3481 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     3541 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3601 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3661 tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3721 atcagctcat ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3781 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3841 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3901 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3961 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4021 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4081 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     4141 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4201 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4261 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4321 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4381 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4441 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4501 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4561 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4621 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4681 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4741 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4801 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4861 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4921 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4981 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5041 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5101 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5161 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5401 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5941 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6001 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6061 gcgcccaata cgcaaaccg
//
