LOCUS       sequence-238493-        9692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Kopec CD, Li B, Wei W, Boehm J, Malinow R
  TITLE     Glutamate receptor exocytosis and spine enlargement during 
            chemically induced long-term potentiation.
  JOURNAL   J Neurosci. 2006 Feb 15. 26(7):2000-9.
  PUBMED    16481433
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2006 Feb 15. 26(7):2000-9."
COMMENT     Sequence Label: pCI-SEP_NR2B
FEATURES             Location/Qualifiers
     source          1..9692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1197..1907
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1224..1252)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1847..1867
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    6806..6927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6817..6836)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6871..6890
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      7108..7563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7195..7214)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7405..7426
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7630..7649)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7749..7771
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7809..7827)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7895..7999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8000..8068,8069..8860)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8218..8237)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9031..9619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9520..9539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagaggt ttggcgtcta caaaccaaga
     1081 gagccgacta gctgaagatg aagcccagcg cagagtgctg ttcccccaag ttctggttgg
     1141 tgttggccgt cttggccgta tcaggcagca aagctcgttc ccaaaagagc gctaggagta
     1201 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     1261 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     1321 cccttaaatt tatttgcact actggaaaac tacctgttcc ttggccaaca cttgtcacta
     1381 ctttaactta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact
     1441 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1501 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     1561 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1621 acaactataa cgatcaccag gtgtacatca tggcagacaa acaaaagaat ggaatcaaag
     1681 ctaacttcaa aattagacac aacattgaag atggaggcgt tcaactagca gaccattatc
     1741 aacaaaatac tccaattggc gatgggcccg tccttttacc agacaaccat tacctgttta
     1801 caacttctac tctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1861 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaagct agccccccca
     1921 gcatcggcat cgctgtcatc ctcgtgggca cttcagacga agtggccata aaagacgccc
     1981 acgagaaaga tgacttccat catctctcag tagttccccg ggtggagctg gtagccatga
     2041 acgaaactga cccaaagagc atcatcaccc gtatctgcga tcttatgtct gaccggaaga
     2101 tccagggggt ggtgttcgcg gatgacaccg accaagaagc catcgctcag atcctcgact
     2161 tcatttctgc tcagactctc acccccatcc tgggcatcca tgggggctca tctatgataa
     2221 tggcggataa ggatgagtcc tccatgttct tccagtttgg cccgtctatc gaacagcaag
     2281 cttccgtcat gctcaacatc atggaagaat atgactggta catcttttcc atcgtcacca
     2341 cctacttccc tggctaccag gactttgtga acaagatccg cagtaccatc gagaacagct
     2401 tcgtgggctg ggagctcgag gaagtcctcc tgctagacat gtctctggac gatggcgact
     2461 ctaagattca gaatcagctg aagaagctcc aaagccccat cattctcctt tattgcacga
     2521 aggaggaagc cacctacatt tttgaagtag ctaactcagt tgggctgact ggctacggct
     2581 acacgtggat tgtgccgagt ctggtggccg gggatacgga cacggtgcct tcagagttcc
     2641 ccacggggct tatctctgtg tcttatgatg aatgggacta tggccttcct gccagagtga
     2701 gagatggaat tgccatcatc accactgctg cctcggacat gctgtccgaa cacagtttca
     2761 tccctgagcc caagagcagt tgctacaaca cccacgagaa gaggatctac cagtctaaca
     2821 tgttgaatag gtatctgatc aatgtcactt ttgaagggag aaacctgtcc ttcagcgaag
     2881 atggctacca gatgcatccg aagctggtga taatccttct gaacaaggag aggaagtggg
     2941 agagggtggg gaaatggaag gacaagtccc tgcagatgaa gtattatgtg tggcctcgga
     3001 tgtgtcctga gactgaggag caagaggatg accatctgag cattgtcacc ttggaggagg
     3061 cgccatttgt cattgtggaa agcgtggacc ctctcagtgg aacctgcatg aggaatacag
     3121 tcccgtgcca gaagcgcatc atctctgaga ataaaacaga tgaggaacca ggctacatca
     3181 aaaaatgctg caaggggttc tgtattgaca tccttaagaa aatttctaag tctgtgaagt
     3241 tcacctatga cctttacctg gtgaccaatg gcaagcacgg gaagaagatt aatgggacct
     3301 ggaatggcat gatcggtgag gtggtcatga agagggccta catggcagtg ggatcactaa
     3361 ctatcaatga agaacggtca gaggtggttg acttctctgt acccttcata gaaactggca
     3421 tcagtgtcat ggtatctcgc agcaatggga ctgtgtcacc ttctgccttc ttagagccat
     3481 tcagcgctga cgtgtgggtg atgatgtttg tgatgctgct cattgtttct gcggtggctg
     3541 tctttgtctt tgaatacttc agccctgtgg gttacaacag gtgcctagcc gatggcagag
     3601 agccaggagg cccatctttc accatcggca aagcaatttg gttactctgg ggtctggtgt
     3661 ttaacaactc cgtacctgtg cagaacccaa aggggaccac ctccaagatc atggtgtcag
     3721 tgtgggcctt ctttgctgtc attttcctgg ccagctacac tgccaactta gcagccttca
     3781 tgatccaaga ggagtatgtg gaccaggttt ctggcctgag tgacaagaag ttccagagac
     3841 ctaatgactt ctcaccccct ttccgctttg ggactgtgcc caatggcagc acagagagga
     3901 atatccgtaa taactatgca gaaatgcatg cctacatggg aaagttcaac caaaggggtg
     3961 tagatgatgc attgctctcc ctgaaaacag ggaagcttga tgcattcatc tatgatgcag
     4021 ctgtgctcaa ctacatggct ggaagggacg aaggctgcaa actggtgacc attggcagtg
     4081 gcaaggtctt tgcttctacc ggctatggca ttgctatcca aaaggactcc gggtggaagc
     4141 gccaggtgga cctggctatc ctgcagctgt ttggagatgg ggagatggaa gaactggaag
     4201 ctctctggct cactggcatt tgccacaatg agaagaatga ggtgatgagc agccagctgg
     4261 acatcgacaa tatggcaggt gtcttctata tgttgggggc agccatggcc ctcagcctca
     4321 tcaccttcat ctgtgagcat ctgttctatt ggcagttccg gcattgcttc atgggtgtct
     4381 gttctggcaa gcctggcatg gtcttctcca tcagcagagg tatctacagc tgtatccatg
     4441 gggtagccat agaggagcgc caatccgtga tgaactcccc cactgccacc atgaacaaca
     4501 cccactccaa catcctacgc ttgctccgca cggccaagaa catggccaac ctgtctggag
     4561 taaacggctc ccctcagagt gccctggact tcatccgccg agagtcctcc gtctacgaca
     4621 tctctgagca tcgtcgcagc ttcacgcatt cagactgcaa gtcttacaat aacccaccct
     4681 gtgaggaaaa cctgttcagt gactacatta gcgaggtaga gagaacattt ggtaacctgc
     4741 agctgaagga cagcaatgtg taccaagacc actatcacca tcaccaccgg ccacacagca
     4801 tcggcagcac cagctccatt gatgggctct atgactgtga caacccaccc ttcaccaccc
     4861 agcccaggtc aatcagcaag aaacccctgg acatcggcct gccctcctcc aaacatagcc
     4921 agctcagcga cctgtatggc aagttctctt tcaagagtga ccgctacagt ggccacgacg
     4981 acttgattcg atcggatgtc tccgacatct ccacgcacac tgtcacctat gggaacatcg
     5041 agggcaacgc agccaagagg aggaaacagc agtataagga cagtctaaag aagcggccag
     5101 cctcggccaa atcgaggagg gagtttgatg aaatcgagct ggcctaccgt cgccgaccac
     5161 cccgctcccc ggaccacaag cgctacttca gggacaaaga agggctccga gacttctacc
     5221 tggaccagtt ccgaacaaag gagaactcgc ctcactggga gcacgtggac ttgactgaca
     5281 tttacaaaga acgcagtgac gacttcaagc gagattcggt cagtggaggt gggccctgta
     5341 ccaacaggtc tcacctcaaa cacggaacgg gcgagaagca cggagtggta ggcggggtgc
     5401 ctgctccttg ggagaagaac ctgaccaatg tggattggga ggaccggtct gggggcaact
     5461 tctgccgcag ctgtccttcc aagctgcaca attactcctc gacggtggca gggcagaact
     5521 cgggccggca ggcctgcatc agatgtgagg cctgtaagaa ggctggtaac ctgtacgaca
     5581 tcagcgagga caactccctg caggaactgg accagccggc tgcccccgtg gctgtgacat
     5641 ccaacgcctc cagcaccaag taccctcaaa gcccgactaa ttccaaggct cagaagaaga
     5701 atcggaacaa actgcgccgg cagcattcct acgacacctt cgtggacctg cagaaggagg
     5761 aggccgcctt ggccccacgc agcgtgagcc tgaaagacaa gggccgattc atggatggga
     5821 gcccctacgc ccatatgttt gagatgccag ctggtgagag ctcctttgcc aacaagtcct
     5881 cagtgcccac tgccggacac caccacaaca accccggcag cggctacatg ctcagcaagt
     5941 cgctctaccc tgaccgggtc acgcaaaacc ctttcatccc cacttttggg gatgaccagt
     6001 gcttgcttca cggcagcaaa tcctacttct tcaggcagcc cacggtggca ggggcgtcaa
     6061 aaacaaggcc ggacttccgg gcccttgtca ccaataagcc agtggtgtca gcccttcatg
     6121 gggctgtgcc aggtcgtttc cagaaggaca tttgtatagg gaaccagtcc aacccctgtg
     6181 tgcctaacaa caaaaacccc agggctttca atggctccag caatggacat gtttatgaga
     6241 aactttctag tattgagtct gatgtctgag tgagggaaga gagagaggtt aaggtgggta
     6301 cgggagggat agggctgtgg gccgcgtggt gcgcatgtca tggaaagagt cgggggtgaa
     6361 cttggctccc attcgctttt tcttcttctt ttaatttctc tatgggatcc tggagttctg
     6421 gttccttact gaaggcaacc ctcgtggcca gcaccatttc tcctccctcg cgcagttctc
     6481 tccttcccgc atctgtccac cattcctgtt tccatgagag aatagaacgg ggcctcagtg
     6541 tgggaggatg gagaggagac caaagcgctg cctgtgtgct tctttctagc gcagaagggc
     6601 tcttagcagt tcactttgag cgaggctttc ctgatgctgc tctctttgtt caggtgagga
     6661 agcgaaggtg ttctgaggaa ggccattgaa cctcctgttc ataagagaga agaggctttc
     6721 actgaattct tgccgcgtgg ttgacctcga gggggggccc ggtacctcta gagtcgaccc
     6781 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     6841 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6901 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6961 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     7021 atcgataagg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7081 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     7141 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7201 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7261 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7321 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7381 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7441 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7501 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7561 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     7621 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     7681 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7741 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     7801 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7861 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7921 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7981 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8041 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8101 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8161 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8221 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8281 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8341 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8401 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8461 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8521 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8581 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8641 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8701 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8761 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8821 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8881 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8941 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9001 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9061 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9661 ctggcctttt gctcacatgg ctcgacagat ct
//
