LOCUS       sequence-238501-        9784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, PGK 
            promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Neo DEST (w531-1)
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..537
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     456..474
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    592..716
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(741..746,747..764,765..771)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             825..1484
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(891..910)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1826..2131
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2010..2029
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2172..2296)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2327..2915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2380..2400)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2798..2809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2958..3457
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2993..3016)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3390..3409
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3471..4274
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3534..3553)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4144..4163
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4365..4598
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4670..4791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4707..4726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4761..4780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4831..4966
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4986..5005)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4987..5005)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5015..5032)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5015..5031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5024..5046)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5173..5628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5260..5279)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5470..5491
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5654..5758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5759..5827,5828..6619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5977..5996)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6790..7378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7279..7298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7532..7549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7666..7687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7702..7707,7708..7725,7726..7732)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7740..7756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7745..7767
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7764..7780
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7764..7780
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7799..7819
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7801..7819
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7847..8073
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8074..8254
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8301..8426
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8919..9152
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9337..9381
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9648..9765
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgagaagct tgatatcgaa ttccacgggg ttggggttgc gccttttcca aggcagccct
       61 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
      121 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
      181 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
      241 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
      301 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
      361 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
      421 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
      481 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
      541 gatccaccgg ttcgtcgact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
      601 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
      661 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
      721 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
      781 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      841 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      901 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      961 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1021 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     1081 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1141 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1201 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     1261 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1321 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1381 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     1441 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     1501 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     1561 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     1621 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     1681 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     1741 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     1801 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     1861 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     1921 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     1981 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     2041 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     2101 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     2161 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     2221 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     2281 tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgacaatc aacctctgga
     2341 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2401 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2461 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2521 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2581 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2641 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2701 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     2761 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2821 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2881 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctgcaga tatccggact
     2941 agtgatctaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     3001 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     3061 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     3121 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     3181 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     3241 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     3301 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     3361 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     3421 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaat atgggatcgg
     3481 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     3541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3661 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     4021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     4201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     4261 acgagttctt ctgaggggat ccgtcgaggg cccggtacct ttaagaccaa tgacttacaa
     4321 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca
     4381 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4441 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4501 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4561 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4621 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4741 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4801 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4861 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4921 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     4981 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5041 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5101 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5161 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5221 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5281 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5341 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5401 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5461 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5521 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5581 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6001 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6301 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6901 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7441 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7501 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7561 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7621 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7681 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7741 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7801 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     7861 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     7921 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     7981 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     8041 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     8101 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8161 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8221 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8281 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8341 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8401 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8461 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8521 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8581 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8641 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8701 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8761 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8821 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8881 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8941 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9001 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9061 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9121 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9181 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9241 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9301 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9361 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9421 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9481 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9541 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     9601 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     9661 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9721 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
     9781 agcc
//
