LOCUS       sequence-238522-        8572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, PGK promoter, Puro .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK GFP Puro (w509-5)
FEATURES             Location/Qualifiers
     source          1..8572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..523
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     442..460
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      539..548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(545..547,548..550,551..1264)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(545..547,548..550,551..1261)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(590..611)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(851..870)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1198..1219
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1280..1868
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1333..1353)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1751..1762)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1882..2381
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1917..1940)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2314..2333
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2402..3001
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2402..2421)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2898..2918
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3139..3372
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3444..3565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3481..3500)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3535..3554
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3605..3740
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3760..3779)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3761..3779)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3789..3806)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3789..3805)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3798..3820)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3947..4402
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4034..4053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4244..4265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4428..4532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4533..4601,4602..5393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4751..4770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5564..6152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6053..6072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6306..6323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6440..6461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6476..6481,6482..6499,6500..6506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6514..6530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6519..6541
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6538..6554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6538..6554
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6573..6593
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6575..6593
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6621..6847
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6848..7028
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7075..7200
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7693..7926
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8111..8155
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8422..8539
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcgaattcc acggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
       61 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
      121 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
      181 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
      241 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
      301 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
      361 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
      421 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
      481 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggtcgc
      541 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      601 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      661 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      721 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      781 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      841 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      901 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      961 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1021 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1081 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1141 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1201 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1261 gtaaagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     1321 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     1381 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     1441 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     1501 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     1561 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     1621 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     1681 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     1741 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     1801 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     1861 ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     1921 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     1981 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     2041 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     2101 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     2161 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     2221 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     2281 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     2341 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     2401 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     2461 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     2521 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     2581 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     2641 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     2701 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     2761 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     2821 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     2881 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     2941 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     3001 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg
     3061 agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3121 taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt
     3181 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3241 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3301 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3361 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     3421 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     3481 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3541 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     3601 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3661 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3721 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     3781 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3841 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3901 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4141 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4261 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4321 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4381 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4441 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4501 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4561 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4621 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4681 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4741 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4801 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4861 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4921 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4981 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5041 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5101 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5161 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5221 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5281 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5341 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5401 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5461 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5521 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5581 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5641 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5701 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5761 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5821 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5881 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5941 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6001 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6061 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6121 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6181 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6241 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6301 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6361 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6421 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6481 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6541 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6601 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     6661 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     6721 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     6781 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     6841 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6901 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6961 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7021 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7081 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7141 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7201 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7261 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7321 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7381 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     7441 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7501 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     7561 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     7621 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     7681 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     7741 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     7801 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     7861 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     7921 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     7981 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8041 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8101 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     8161 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     8221 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     8281 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     8341 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     8401 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
     8461 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     8521 tacaaaaatt caaaatttta tcgatcacga gactagcctc gagaagcttg at
//
