LOCUS       sequence-238536-        1316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To transform BL21-Gold(DE3) and induced by IPTG for 
            His-proteinA-MNase fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ku WL, Pan L, Cao Y, Gao W, Zhao K
  TITLE     Profiling single-cell histone modifications using indexing chromatin
            immunocleavage sequencing.
  JOURNAL   Genome Res. 2021 Apr 14. pii: gr.260893.120. doi: 
            10.1101/gr.260893.120.
  PUBMED    33853847
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2021 Apr 14. pii: gr.260893.120. doi: 10.1101/gr.260893.120."
COMMENT     Sequence Label: pET15b-pA-Mnase
FEATURES             Location/Qualifiers
     source          1..1316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             22..44
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..107
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             564..989
                     /codon_start=1
                     /product="immunoglobulin binding domains of Staphylocuccus 
                     aureus protein A"
                     /label=protein A
                     /translation="SLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHL
                     PNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLP
                     NLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPK"
ORIGIN
        1 gnnnnntnnc tctagaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca
       61 gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat atgcatgcgc
      121 aacacgatga agctcaacaa aatgcttttt atcaagtctt aaatatgcct aacttaaatg
      181 ctgatcaacg caatggtttt atccaaagcc ttaaagatga tccaagccaa agtgctaacg
      241 ttttaggtga agctaaaaaa cttaatgact ctcaagctcc aaaagctgat gcgcaacaaa
      301 ataacttcaa caaagatcaa caaagcgcct tctatgaaat tttgaacatg cctaacttaa
      361 acgaagcgca acgcaatggt ttcattcaaa gtcttaaaga cgatccaagc caaagcacta
      421 acgttttagg tgaagctaaa aaattaaacg aatctcaagc accgaaagct gacaacaatt
      481 tcaacaaaga acaacaaaat gctttctatg aaatcttgaa catgcctaac ttgaacgaag
      541 aacaacgcaa tggtttcatc caaagcttaa aagatgaccc aagtcaaagt gctaacctat
      601 tgtcagaagc taaaaagtta aatgaatctc aagcaccgaa agcggataac aaattcaaca
      661 aagaacaaca aaatgctttc tatgaaatct tacatttacc taacttaaac gaagaacaac
      721 gcaatggttt catccaaagc ttaaaagatg acccaagcca aagcgctaac cttttagcag
      781 aagctaaaaa gctaaatgat gcacaagcac caaaagctga caacaaattc aacaaagaac
      841 aacaaaatgc tttctatgaa attttacatt tacctaactt aactgaagaa caacgtaacg
      901 gcttcatcca aagccttaaa gacgatcctt cagtgagcaa agaaatttta gcagaagcta
      961 aaaagctaaa cgatgctcaa gcaccaaaag gnggncggtg aattctcaca aacagataac
     1021 ggcgtaaata gaagtggttc tgaagatcca acagtatata gtgcaacttc aactaaaaaa
     1081 ttacataaag aacctgcgac attaattaaa gcgattgatg gtgatacggt taaattaatg
     1141 tacaaaggtc aaccatggan nttcanncta ttattgggtt ganncccntg aaacaangca
     1201 tcctaaaaaa gggnnaanna annntggntc cngaanccag nnncnttncc nanaaanngg
     1261 naaaanngnc aaaaaaantn naattccagt ttgnnaaggn cnaanaannn naaant
//
