LOCUS       sequence-238537-        1292 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To transform BL21-Gold(DE3) and induced by IPTG for 
            His-proteinA-MNase fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ku WL, Pan L, Cao Y, Gao W, Zhao K
  TITLE     Profiling single-cell histone modifications using indexing chromatin
            immunocleavage sequencing.
  JOURNAL   Genome Res. 2021 Apr 14. pii: gr.260893.120. doi: 
            10.1101/gr.260893.120.
  PUBMED    33853847
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2021 Apr 14. pii: gr.260893.120. doi: 10.1101/gr.260893.120."
COMMENT     Sequence Label: pET15b-pA-Mnase
FEATURES             Location/Qualifiers
     source          1..1292
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..481)
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ"
     CDS             complement(554..979)
                     /codon_start=1
                     /product="immunoglobulin binding domains of Staphylocuccus 
                     aureus protein A"
                     /label=protein A
                     /translation="SLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHL
                     PNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLP
                     NLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPK"
ORIGIN
        1 cnnctttcgg gctttgttag cagccggatc cttattgacc tgaatcagcg ttgtcttcgc
       61 tccaaatatt taatttctct ttttttgctt gtgcttcact ttttcttaaa agttgttcat
      121 gtgtattgtt aggtttataa acataagcaa ctttagccaa gccttgacga actaaagctt
      181 cgtttaccat ttttccatca gcataaatat acgctaagcc acgtccatat ttatcagttc
      241 tttgaccttt gtcaaactcg acttcaattt tctttgcatt ttctaccatt tttttcgtaa
      301 atgcacttgc ttcaggacca tatttctcta cacctttttt aggatgcttt gtttcaggtg
      361 tatcaaccaa taatagtctg aatgtcattg gttgaccttt gtacattaat ttaaccgtat
      421 caccatcaat cgctttaatt aatgtcgcag gttctttatg taatttttta gttgaagttg
      481 cactatatac tgttggatct tcagaaccac ttctatttac gccgttatct gtttgtgaga
      541 attcaccgcc accttttggt gcttgagcat cgtttagctt tttagcttct gctaaaattt
      601 ctttgctcac tgaaggatcg tctttaaggc tttggatgaa gccgttacgt tgttcttcag
      661 ttaagttagg taaatgtaaa atttcataga aagcattttg ttgttctttg ttgaatttgt
      721 tgtcagcttt tggtgcttgt gcatcattta gctttttagc ttctgctaaa aggttagcgc
      781 tttggcttgg gtcatctttt aagctttgga tgaaaccatt gcgttgttct tcgtttaagt
      841 taggtaaatg taagatttca tagaaagcat tttgttgttc tttgttgaat ttgttatccg
      901 ctttcggtgc ttgagattca tttaactttt tagcttctga caataggtta gcactttgac
      961 ttgggtcatc ttttaagctt tggatgaaac cattgcgttg ttcttcgttc aagttaggca
     1021 tgttcaagat ttcatagaaa gcattttgtt gttctttgtt gaaattgttg tcagctttcg
     1081 gtgcttgnna ttcgtttaat tttttagctt cacctaaaac gttagtgctt tggnctggna
     1141 tcgtntttaa nactttgaat gaaaccattg nngttgnnct tcgtttaagt taggcngntt
     1201 caaaatttcn tanaaggnnn ttnnttgnat ttnnnttgaa ntnntttnnn ttggnnnnnn
     1261 cnttttggnn ncttgnaann nnnnaangtt tt
//
