LOCUS       sequence-238539-       13004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human type I fatty acid synthase (FASN) in Escherichia 
            coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M
  TITLE     Probing the modularity of megasynthases by rational engineering of a
            fatty acid synthase Type I.
  JOURNAL   Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 
            2018 Dec 20.
  PUBMED    30394635
REFERENCE   2  (bases 1 to 13004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3550"; 
            journalName: "Protein Sci"; date: "2019-02"; volume: "28"; issue: 
            "2"; pages: "414-428"
COMMENT     Sequence Label: pAR17_StrepI_hFASh_H8_pET22b
FEATURES             Location/Qualifiers
     source          1..13004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     975..994
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1014..1091)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1297..1316
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1400..1419
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1400..1418
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1419..1443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1458..1480
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(1491..1496,1497..1520,1521..1556,1557..1580)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             9129..9152
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      9219..9266
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9303..9758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9390..9409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9600..9621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9784..9888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9889..9957,9958..10749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10107..10126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10920..11508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11409..11428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11662..11679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11694..11833
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11759..11778)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11919..11941
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11935..12126)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(12935..13004,1..1013))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
       61 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
      121 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
      181 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
      241 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
      301 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
      361 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
      421 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
      481 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
      541 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
      601 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
      661 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
      721 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
      781 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
      841 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
      901 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
      961 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     1021 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     1081 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     1141 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     1201 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     1261 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     1321 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     1381 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     1441 tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg agcgcttgga
     1501 gccatccaca atttgagaag ggtggaggtt ctggcggtgg atcgggaggt tcagcgtgga
     1561 gccacccgca gttcgaaaaa ggcgccggat ccgaggaggt ggtgattgcc ggcatgtccg
     1621 ggaagctgcc agagtcggag aacttgcagg agttctggga caacctcatc ggcggtgtgg
     1681 acatggtcac ggacgatgac cgtcgctgga aggcggggct ctacggcctg ccccggcggt
     1741 ccggcaagct gaaggacctg tctaggtttg atgcctcctt cttcggagtc caccccaagc
     1801 aggcacacac gatggaccct cagctgcggc tgctgctgga agtcacctat gaagccatcg
     1861 tggacggagg catcaaccca gattcactcc gaggaacaca cactggcgtc tgggtgggcg
     1921 tgagcggctc tgagacctcg gaggccctga gccgagaccc cgagacactc gtgggctaca
     1981 gcatggtggg ctgccagcga gcgatgatgg ccaaccggct ctccttcttc ttcgacttca
     2041 gagggcccag catcgcactg gacacagcct gctcctccag cctgatggcc ctgcagaacg
     2101 cctaccaggc catccacagc gggcagtgcc ctgccgccat cgtggggggc atcaatgtcc
     2161 tgctgaagcc caacacctcc gtgcagttct tgaggctggg gatgctcagc cccgagggca
     2221 cctgcaaggc cttcgacaca gcggggaatg ggtactgccg ctcggagggt gtggtggccg
     2281 tcctgctgac caagaagtcc ctggcccggc gggtgtacgc caccatcctg aacgccggca
     2341 ccaatacaga tggcttcaag gagcaaggcg tgaccttccc ctcaggggat atccaggagc
     2401 agctcatccg ctcgttgtac cagtcggccg gagtggcccc tgagtcattt gaatacatcg
     2461 aagcccacgg cacaggcacc aaggtgggcg acccccagga gctgaatggc atcacccgag
     2521 ccctgtgcgc cacccgccag gagccgctgc tcatcggctc caccaagtcc aacatggggc
     2581 acccggagcc agcctcgggg ctggcagccc tggccaaggt gctgctgtcc ctggagcacg
     2641 ggctctgggc ccccaacctg cacttccata gccccaaccc tgagatccca gcgctgttgg
     2701 atgggcggct gcaggtggtg gaccagcccc tgcccgtccg tggcggcaac gtgggcatca
     2761 actcctttgg cttcgggggc tccaacgtgc acatcatcct gaggcccaac acgcagccgc
     2821 cccccgcacc cgccccacat gccaccctgc cccgtctgct gcgggccagc ggacgcaccc
     2881 ctgaggccgt gcagaagctg ctggagcagg gcctccggca cagccaggac ctggctttcc
     2941 tgagcatgct gaacgacatc gcggctgtcc ccgccaccgc catgcccttc cgtggctacg
     3001 ctgtgctggg tggtgagcgc ggtggcccag aggtgcagca ggtgcccgct ggcgagcgcc
     3061 cgctctggtt catctgctct gggatgggca cacagtggcg cgggatgggg ctgagcctca
     3121 tgcgcctgga ccgcttccga gattccatcc tacgctccga tgaggctgtg aagccattcg
     3181 gcctgaaggt gtcacagctg ctgctgagca cagacgagag cacctttgat gacatcgtcc
     3241 attcgtttgt gagcctgact gccatccaga taggcctcat agacctgctg agctgcatgg
     3301 ggctgaggcc agatggcatc gtcggccact ccctggggga ggtggcctgt ggctacgccg
     3361 acggctgcct gtcccaggag gaggccgtcc tcgctgccta ctggagggga cagtgcatca
     3421 aagaagccca tctcccgccg ggcgccatgg cagccgtggg cttgtcctgg gaggagtgta
     3481 aacagcgctg ccccccgggc gtggtgcccg cctgccacaa ctccaaggac acagtcacca
     3541 tctcgggacc tcaggccccg gtgtttgagt tcgtggagca gctgaggaag gagggtgtgt
     3601 ttgccaagga ggtgcggacc ggcggtatgg ccttccactc ctacttcatg gaggccatcg
     3661 cacccccact gctgcaggag ctcaagaagg tgatccggga gccgaagcca cgttcagccc
     3721 gctggctcag cacctctatc cccgaggccc agtggcacag cagcctggca cgcacgtcct
     3781 ccgccgagta caatgtcaac aacctggtga gccctgtgct gttccaggag gccctgtggc
     3841 acgtgcctga gcacgcggtg gtgctggaga tcgcgcccca cgccctgctg caggctgtcc
     3901 tgaagcgtgg cctgaagccg agctgcacca tcatccccct gatgaagaag gatcacaggg
     3961 acaacctgga gttcttcctg gccggcatcg gcaggctgca cctctcaggc atcgacgcca
     4021 accccaatgc cttgttccca cctgtggagt tcccagctcc ccgaggaact cccctcatct
     4081 ccccactcat caagtgggac cacagcctgg cctgggacgt gccggccgcc gaggacttcc
     4141 ccaacggttc aggttccccc tcagccgcca tctacaacat cgacaccagc tccgagtctc
     4201 ctgaccacta cctggtggac cacaccctcg acggtcgcgt cctcttcccc gccactggct
     4261 acctgagcat agtgtggaag acgctggccc gcgccctggg cctgggcgtc gagcagctgc
     4321 ctgtggtgtt tgaggatgtg gtgctgcacc aggccaccat cctgcccaag actgggacag
     4381 tgtccctgga ggtacggctc ctggaggcct cccgtgcctt cgaggtgtca gagaacggca
     4441 acctggtagt gagtgggaag gtgtaccagt gggatgaccc tgaccccagg ctcttcgacc
     4501 acccggaaag ccccaccccc aaccccacgg agcccctctt cctggcccag gctgaagttt
     4561 acaaggagct gcgtctgcgt ggctacgact acggccctca tttccagggc atcctggagg
     4621 ccagcctgga aggtgactcg gggaggctgc tgtggaagga taactgggtg agcttcatgg
     4681 acaccatgct gcagatgtcc atcctgggct cggccaagca cggcctgtac ctgcccaccc
     4741 gtgtcaccgc catccacatc gaccctgcca cccacaggca gaagctgtac acactgcagg
     4801 acaaggccca agtggctgac gtggtggtga gcaggtggct gagggtcaca gtggccggag
     4861 gcgtccacat ctccgggctc cacactgagt cggccccgcg gcggcagcag gagcagcagg
     4921 tgcccatcct ggagaagttt tgcttcactc cccacacgga ggaggggtgc ctgtctgagc
     4981 gcgctgccct gcaggaggag ctgcaactgt gcaaggggct ggtgcaggca ctgcagacca
     5041 cggtgaccca gcaggggctg aagatggtgg tgcccggact ggatggggcc cagatccccc
     5101 gggacccctc acagcaggaa ctgccccggc tgttgtcggc tgcctgcagg cttcagctca
     5161 acgggaacct gcagctggag ctggcgcagg tgctggccca ggagaggccc aagctgccag
     5221 aggaccctct gctcagcggc ctcctggact ccccggcact caaggcctgc ctggacactg
     5281 ccgtggagaa catgcccagc ctgaagatga aggtggtgga ggtgctggct ggccacggtc
     5341 acctgtattc ccgcatccca ggcctgctca gcccccatcc cctgctgcag ctgagctaca
     5401 cggccaccga ccgccacccc caggccctgg aggctgccca ggccgagctg cagcagcacg
     5461 acgttgccca gggccagtgg gatcccgcag accctgcccc cagcgccctg ggcagcgccg
     5521 acctcctggt gtgcaactgt gctgtggctg ccctcgggga cccggcctca gctctcagca
     5581 acatggtggc tgccctgaga gaagggggct ttctgctcct gcacacactg ctccgggggc
     5641 accccctcgg ggacatcgtg gccttcctca cctccactga gccgcagtat ggccagggca
     5701 tcctgagcca ggacgcgtgg gagagcctct tctccagggt gtcgctgcgc ctggtgggcc
     5761 tgaagaagtc cttctacggc tccacgctct tcctgtgccg ccggcccacc ccgcaggaca
     5821 gccccatctt cctgccggtg gacgatacca gcttccgctg ggtggagtct ctgaagggca
     5881 tcctggctga cgaagactct tcccggcctg tgtggctgaa ggccatcaac tgtgccacct
     5941 cgggcgtggt gggcttggtg aactgtctcc gccgagagcc cggcgggaac cgcctccggt
     6001 gtgtgctgct ctccaacctc agcagcacct cccacgtccc ggaggtggac ccgggctccg
     6061 cagaactgca gaaggtgttg cagggagacc tggtgatgaa cgtctaccgc gacggggcct
     6121 ggggggcttt ccgccacttc ctgctggagg aggacaagcc tgaggagccg acggcacatg
     6181 cctttgtgag caccctcacc cggggggacc tgtcctccat ccgctgggtc tgctcctcgc
     6241 tgcgccatgc ccagcccacc tgccctggcg cccagctctg cacggtctac tacgcctccc
     6301 tcaacttccg cgacatcatg ctggccactg gcaagctgtc ccctgatgcc atcccaggga
     6361 agtggacctc ccaggacagc ctgctaggta tggagttctc gggccgagac gccagcggca
     6421 agcgtgtgat gggactggtg cctgccaagg gcctggccac ctctgtcctg ctgtcaccgg
     6481 acttcctctg ggatgtgcct tccaactgga cgctggagga ggcggcctcg gtgcctgtcg
     6541 tctacagcac ggcctactac gcgctggtgg tgcgtgggcg ggtgcgcccc ggggagacgc
     6601 tgctcatcca ctcgggctcg ggcggcgtgg gccaggccgc catcgccatc gccctcagtc
     6661 tgggctgccg cgtcttcacc accgtggggt cggctgagaa gcgggcgtac ctccaggcca
     6721 ggttccccca gctcgacagc accagcttcg ccaactcccg ggacacatcc ttcgagcagc
     6781 atgtgctgtg gcacacgggc gggaagggcg ttgacctggt cttgaactcc ttggcggaag
     6841 agaagctgca ggccagcgtg aggtgcttgg ctacgcacgg tcgcttcctg gaaattggca
     6901 aattcgacct ttctcagaac cacccgctcg gcatggctat cttcctgaag aacgtgacat
     6961 tccacggggt cctactggat gcgttcttca acgagagcag tgctgactgg cgggaggtgt
     7021 gggcgcttgt gcaggccggc atccgggatg gggtggtacg gcccctcaag tgcacggtgt
     7081 tccatggggc ccaggtggag gacgccttcc gctacatggc ccaagggaag cacattggca
     7141 aagtcgtcgt gcaggtgctt gcggaggagc cggaggcagt gttgaagggg gccaaaccca
     7201 agctgatgtc ggccatctcc aagaccttct gcccggccca caagagctac atcatcgctg
     7261 gtggtctggg tggcttcggc ctggagttgg cgcagtggct gatacagcgt ggggtgcaga
     7321 agctcgtgtt gacttctcgc tccgggatcc ggacaggcta ccaggccaag caggtccgcc
     7381 ggtggaggcg ccagggcgta caggtgcagg tgtccaccag caacatcagc tcactggagg
     7441 gggcccgggg cctcattgcc gaggcggcgc agcttgggcc cgtgggcggc gtcttcaacc
     7501 tggccgtggt cttgagagat ggcttgctgg agaaccagac cccagagttc ttccaggacg
     7561 tctgcaagcc caagtacagc ggcaccctga acctggacag ggtgacccga gaggcgtgcc
     7621 ctgagctgga ctactttgtg gtcttctcct ctgtgagctg cgggcgtggc aatgcgggac
     7681 agagcaacta cggctttgcc aattccgcca tggagcgtat ctgtgagaaa cgccggcacg
     7741 aaggcctccc aggcctggcc gtgcagtggg gcgccatcgg cgacgtgggc attttggtgg
     7801 agacgatgag caccaacgac acgatcgtca gtggcacgct gccccagcgc atggcgtcct
     7861 gcctggaggt gctggacctc ttcctgaacc agccccacat ggtcctgagc agctttgtgc
     7921 tggctgagaa ggctgcggcc tatagggaca gggacagcca gcgggacctg gtggaggccg
     7981 tggcacacat cctgggcatc cgcgacttgg ctgctgtcaa cctggacagc tcactggcgg
     8041 acctgggcct ggactcgctc atgagcgtgg aggtgcgcca gacgctggag cgtgagctca
     8101 acctggtgct gtccgtgcgc gaggtgcggc aactcacgct ccggaaactg caggagctgt
     8161 cctcaaaggc ggatgaggcc agcgagctgg catgccccac gcccaaggag gatggtctgg
     8221 cccagcagca gactcagctg aacctgcgct ccctgctggt gaacccggag ggccccaccc
     8281 tgatgcggct caactccgtg cagagctcgg agcggcccct gttcctggtg cacccaatcg
     8341 agggctccac caccgtgttc cacagcctgg cctcccggct cagcatcccc acctatggcc
     8401 tgcagtgcac ccgagctgcg ccccttgaca gcatccacag cctggctgcc tactacatcg
     8461 actgcatcag gcaggtgcag cccgagggcc cctaccgcgt ggccggctac tcctacgggg
     8521 cctgcgtggc ctttgaaatg tgctcccagc tgcaggccca gcagagccca gcccccaccc
     8581 acaacagcct cttcctgttc gacggctcgc ccacctacgt actggcctac acccagagct
     8641 accgggcaaa gctgacccca ggctgtgagg ctgaggctga gacggaggcc atatgcttct
     8701 tcgtgcagca gttcacggac atggagcaca acagggtgct ggaggcgctg ctgccgctga
     8761 agggcctaga ggagcgtgtg gcagccgccg tggacctgat catcaagagc caccagggcc
     8821 tggaccgcca ggagctgagc tttgcggccc ggtccttcta ctacaagctg cgtgccgctg
     8881 agcagtacac acccaaggcc aagtaccatg gcaacgtgat gctactgcgc gccaagacgg
     8941 gtggcgccta cggcgaggac ctgggcgcgg actacaacct ctcccaggta tgcgacggga
     9001 aagtatccgt ccacgtcatc gagggtgacc accgcacgct gctggagggc agcggcctgg
     9061 agtccatcat cagcatcatc cacagctccc tggctgagcc acgcgtgagc gtgcgggagg
     9121 gcctcgagca tcatcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
     9181 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     9241 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
     9301 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     9361 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9421 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9481 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     9541 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     9601 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     9661 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     9721 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     9781 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta ttcgctcatg
     9841 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9901 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9961 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    10021 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    10081 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    10141 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    10201 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    10261 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    10321 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    10381 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
    10441 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    10501 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    10561 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    10621 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10681 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10741 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10801 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10861 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10921 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10981 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11041 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    11101 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11161 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    11221 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    11281 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    11341 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    11401 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    11461 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    11521 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    11581 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    11641 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
    11701 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta
    11761 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
    11821 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    11881 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    11941 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
    12001 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
    12061 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
    12121 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
    12181 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
    12241 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
    12301 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
    12361 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
    12421 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
    12481 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
    12541 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
    12601 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
    12661 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
    12721 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
    12781 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
    12841 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
    12901 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    12961 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg ggga
//
