LOCUS       sequence-238550-       11339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid for expression of SAD B19 G and 
            mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 11339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: pLV-TTBG
FEATURES             Location/Qualifiers
     source          1..11339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     300..324
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             410..1030
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             1184..1240
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1241..1939
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             1949..2005
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    3590..4178
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3643..3663)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4061..4072)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4588..4768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4794..4811)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4800..5024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5070..5498
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5157..5176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5367..5388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5507..5527)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5512..5841
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5692..5827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5754..5773
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5889..5936
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5955..6329
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6459..6580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6496..6515)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6550..6569
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6629..6645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6629..6645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6642..6664)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6653..6669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6677..6683,6684..6701,6702..6707))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6722..6743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6860..6877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7031..7619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7111..7130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7790..8581,8582..8650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8413..8432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8651..8755)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8830..8849)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9021..9400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9402..9600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9555..9575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9618..9798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9845..9970
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10463..10696
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10881..10925
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11177..11294
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(11336..11339,1..15,16..38,39..57,58..80,81..99,
                     100..122,123..141,142..164,165..183,184..206,207..225,
                     226..248,249..267)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
       61 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      121 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      181 ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
      241 ttcacttttc tctatcactg atagggagtg gtaaaggatc cctcgaccta tataagcaga
      301 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      361 agaagacacc gggaccgatc cagcctccgc ggccctgcag gccgccacca tgtccagact
      421 ggacaagagc aaagtcatca actctgcctt ggagctcctg aatgaagttg gcattgaggg
      481 cttgaccacc aggaagctgg cccagaagct gggtgtggag cagcctaccc tgtactggca
      541 tgtgaagaac aagagggctc tgcttgatgc cctggccatt gagatgttgg acaggcacca
      601 cacccacttc tgccctctgg aaggggagtc ctggcaggac ttcctgagga acaatgccaa
      661 gagcttcaga tgtgccttgc tctcccaccg ggatggtgcc aaagttcact tgggcaccag
      721 gcctacagag aagcagtatg agaccctgga gaaccagctg gcattcctgt gccaacaagg
      781 cttctccctg gaaaatgcct tgtatgccct ctctgctgtg ggccacttca ccttgggctg
      841 tgtgctggag gaccaggagc accaagttgc caaggaggag agggagaccc ccaccactga
      901 ctccatgcca ccactgctgc ggcaagctat tgagttgttt gaccaccaag gggctgagcc
      961 tgcattcctt tttggcctgg aactgatcat ctgtggcctg gaaaagcagc tgaaatgtga
     1021 gtctggctct cacgtgccca aaaagagaaa gcacgtgcct gctgatgcct tggatgattt
     1081 tgacctggac atgctgcctg ctgatgccct ggatgacttt gatttggaca tgctccctgc
     1141 tgatgcactt gatgattttg acctggatat gctgggaagc ggagctacta acttcagcct
     1201 gctgaagcag gctggagacg tggaggagaa ccctggacct atgagcgagc tgattaagga
     1261 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     1321 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     1381 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     1441 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     1501 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     1561 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     1621 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     1681 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     1741 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
     1801 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     1861 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     1921 actggggcac aagcttaatg gaagcggagc tactaacttc agcctgctga agcaggctgg
     1981 agacgtggag gagaaccctg gacctatggt tcctcaggct ctcctgtttg taccccttct
     2041 ggtttttcca ttgtgttttg ggaaattccc tatttacacg ataccagaca agcttggtcc
     2101 ctggagtccg attgacatac atcacctcag ctgcccaaac aatttggtag tggaggacga
     2161 aggatgcacc aacctgtcag ggttctccta catggaactt aaagttggat acatcttagc
     2221 cataaaagtg aacgggttca cttgcacagg cgttgtgacg gaggctgaaa cctacactaa
     2281 cttcgttggt tatgtcacaa ccacgttcaa aagaaagcat ttccgcccaa caccagatgc
     2341 atgtagagcc gcgtacaact ggaagatggc cggtgacccc agatatgaag agtctctaca
     2401 caatccgtac cctgactacc gctggcttcg aactgtaaaa accaccaagg agtctctcgt
     2461 tatcatatct ccaagtgtgg cagatttgga cccatatgac agatcccttc actcgagggt
     2521 cttccctagc gggaagtgct caggagtagc ggtgtcttct acctactgct ccactaacca
     2581 cgattacacc atttggatgc ccgagaatcc gagactaggg atgtcttgtg acatttttac
     2641 caatagtaga gggaagagag catccaaagg gagtgagact tgcggctttg tagatgaaag
     2701 aggcctatat aagtctttaa aaggagcatg caaactcaag ttatgtggag ttctaggact
     2761 tagacttatg gatggaacat gggtctcgat gcaaacatca aatgaaacca aatggtgccc
     2821 tcccgataag ttggtgaacc tgcacgactt tcgctcagac gaaattgagc accttgttgt
     2881 agaggagttg gtcaggaaga gagaggagtg tctggatgca ctagagtcca tcatgacaac
     2941 caagtcagtg agtttcagac gtctcagtca tttaagaaaa cttgtccctg ggtttggaaa
     3001 agcatatacc atattcaaca agaccttgat ggaagccgat gctcactaca agtcagtcag
     3061 aacttggaat gagatcctcc cttcaaaagg gtgtttaaga gttgggggga ggtgtcatcc
     3121 tcatgtgaac ggggtgtttt tcaatggtat aatattagga cctgacggca atgtcttaat
     3181 cccagagatg caatcatccc tcctccagca acatatggag ttgttggaat cctcggttat
     3241 cccccttgtg caccccctgg cagacccgtc taccgttttc aaggacggtg acgaggctga
     3301 ggattttgtt gaagttcacc ttcccgatgt gcacaatcag gtctcaggag ttgacttggg
     3361 tctcccgaac tgggggaagt atgtattact gagtgcaggg gccctgactg ccttgatgtt
     3421 gataattttc ctgatgacat gttgtagaag agtcaatcga tcagaaccta cgcaacacaa
     3481 tctcagaggg acagggaggg aggtgtcagt cactccccaa agcgggaaga tcatatcttc
     3541 atgggaatca cacaagagtg ggggtgagac cagactgtaa tgagctagca atcaacctct
     3601 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3661 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3721 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3781 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3841 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3901 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3961 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4021 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4081 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4141 tcagacgagt cggatctccc tttgggccgc ctccccgcct gttcgaattc gagctcggta
     4201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4261 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     4321 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     4381 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     4441 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     4501 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     4561 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     4621 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4681 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4741 ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat cagcctcgac
     4801 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4861 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4921 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4981 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5041 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5101 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5161 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5221 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5281 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5341 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5401 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5461 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5521 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5581 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5641 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5761 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5821 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5881 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     5941 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     6001 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     6061 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     6121 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     6181 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     6241 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     6301 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     6361 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6421 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6481 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6541 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6601 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6661 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6721 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6841 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6901 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6961 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7021 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7081 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7141 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7201 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7261 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7321 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7381 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7441 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7501 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7561 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7621 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7681 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7741 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7921 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8641 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8701 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8761 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     8821 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8881 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8941 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     9001 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     9061 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     9121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     9181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     9241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     9301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     9361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     9421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     9541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9601 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9661 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9721 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9781 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     9841 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     9901 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     9961 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    10021 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    10081 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    10141 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    10201 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    10261 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
    10321 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    10381 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    10441 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    10501 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    10561 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    10621 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    10681 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    10741 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    10801 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    10861 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    10921 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    10981 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    11041 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    11101 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    11161 tagtgaacgg atcaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    11221 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    11281 aaattcaaaa ttttatcgtt ggctcgaccc gggtaccgag ctcgactttc acttttctc
//
