LOCUS       sequence-238556-        1276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To transform BL21-Gold(DE3) and be induced by IPTG for 
            His-proteinG-MNase fusion protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1276)
  TITLE     To transform BL21-Gold(DE3) and be induced by IPTG for 
            His-proteinG-MNase fusion protein expression.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15bHis6-pG-MNase
FEATURES             Location/Qualifiers
     source          1..1276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             740..1186
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQLXRKSEAQAKXEKLNIWSEXNADSGQ"
ORIGIN
        1 ttcccntcta gaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatttga agggtgaaac
      121 gaccacagaa gcagtagacg cggccactgc cgagaaggtg tttaaacaat atgcgaacga
      181 caacggagtc gatggggaat ggacttatga cgatgcaacc aaaactttca ctgttacgga
      241 gaagcccgaa gtaattgacg ccagcgaact tacccccgcg gtaacgacgt ataaacttgt
      301 aattaatggt aagacgctga agggggagac caccactgag gccgtagatg cagctacagc
      361 cgagaaggtc tttaaacagt atgccaatga taatggtgtc gacggtgagt ggacgtacga
      421 tgatgcaact aagacattca ccgtaacaga aaagccggaa gttattgacg cgtcggaact
      481 tactcctgcg gtgaccactt acaaactggt aatcaacggc aagacgctta agggagaaac
      541 aacgactaaa gccgtcgacg ccgaaacggc tgagaaagct ttcaagcaat acgccaatga
      601 taatggggta gacggggttt ggacgtatga tgacgccacc aagactttta cagtcacgga
      661 aatgggtggt ggaatgataa cttcacaaac agataacggc gtaaatagaa gtggttctga
      721 agatccaaca gtatatagtg caacttcaac taaaaaatta cataaagaac ctgcgacatt
      781 aattaaagcg attgatggtg atacggttaa attaatgtac aaaggtcaac caatgacatt
      841 cagactatta ttggttgata cacctgaaac aaagcatcct aaaaaaggtg tagagaaata
      901 tggtcctgaa gcaagtgcat ttacgaaaaa aatggtagaa aatgcaaaga aaattgaagt
      961 cgagtttgac aaaggtcaaa gaactgataa atatggacgt ggcttagcgt atatttatgc
     1021 tgatggaaaa atggtaaacg aagctttagt tcgtcaaggc ttggctaaag ttgcttatgt
     1081 ttataaacct aacaatacac atgaacaact ttnaagaaaa agtgaagcnc aagcaaaaaa
     1141 ngagaaatta aatatttgga gcgaanacaa cgctgattca ggtcaataag gatccggctg
     1201 ctaacaancc cnaanggaan nngaattggn tgntgccccc nntgaacaaa nctannnnaa
     1261 ccnnnggggn nnnaaa
//
