LOCUS       sequence-238557-        1179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To transform BL21-Gold(DE3) and be induced by IPTG for 
            His-proteinG-MNase fusion protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1179)
  TITLE     To transform BL21-Gold(DE3) and be induced by IPTG for 
            His-proteinG-MNase fusion protein expression.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15bHis6-pG-MNase
FEATURES             Location/Qualifiers
     source          1..1179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..479)
                     /codon_start=1
                     /product="micrococcal nuclease from Staphylococcus aureus"
                     /label=MNase
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ"
     CDS             complement(1116..1133)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tnnttcgggc tttgttagca gccggatcct tattgacctg aatcagcgtt gtcttcgctc
       61 caaatattta atttctcttt ttttgcttgt gcttcacttt ttcttaaaag ttgttcatgt
      121 gtattgttag gtttataaac ataagcaact ttagccaagc cttgacgaac taaagcttcg
      181 tttaccattt ttccatcagc ataaatatac gctaagccac gtccatattt atcagttctt
      241 tgacctttgt caaactcgac ttcaattttc tttgcatttt ctaccatttt tttcgtaaat
      301 gcacttgctt caggaccata tttctctaca ccttttttag gatgctttgt ttcaggtgta
      361 tcaaccaata atagtctgaa tgtcattggt tgacctttgt acattaattt aaccgtatca
      421 ccatcaatcg ctttaattaa tgtcgcaggt tctttatgta attttttagt tgaagttgca
      481 ctatatactg ttggatcttc agaaccactt ctatttacgc cgttatctgt ttgtgaagtt
      541 atcattccac cacccatttc cgtgactgta aaagtcttgg tggcgtcatc atacgtccaa
      601 accccgtcta ccccattatc attggcgtat tgcttgaaag ctttctcagc cgtttcggcg
      661 tcgacggctt tagtcgttgt ttctccctta agcgtcttgc cgttgattac cagtttgtaa
      721 gtggtcaccg caggagtaag ttccgacgcg tcaataactt ccggcttttc tgttacggtg
      781 aatgtcttag ttgcatcatc gtacgtccac tcaccgtcga caccattatc attggcatac
      841 tgtttaaaga ccttctcggc tgtagctgca tctacggcct cagtggtggt ctcccccttc
      901 agcgtcttac cattaattac aagtttatac gtcgttaccg cgggggtaag ttcgctggcg
      961 tcaattactt cgggcttctc cgtaacagtg aaagttttgg ttgcatcgtc ataagtccat
     1021 tccccatcga ctccgttgtc gttcgcatat tgtttaaaca ccttctcggc agtggccgcg
     1081 tctactgctt ctgtggtcgt ttcacccttc aaatggctgc cgcgcggnac caggccgctg
     1141 ctggnatgan nannatgatg gctgctgccn atgggannt
//
