LOCUS       sequence-238572-       13636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Binary vector for Agrobacterium tumefacience-mediated 
            transformation of plant cells resulting in constitutive expression 
            of cytoplasmatically localized RS-GFP"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Nocarova E, Fischer L
  TITLE     Cloning of transgenic tobacco BY-2 cells; an efficient method to 
            analyse and reduce high natural heterogeneity of transgene 
            expression.
  JOURNAL   BMC Plant Biol. 2009 Apr 22;9:44. doi: 10.1186/1471-2229-9-44.
  PUBMED    19386122
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2009-04-22"; volume: "9:44. doi"; pages: " 
            10.1186/1471-2229-9-44"
COMMENT     Sequence Label: pCP60 RS-GFP
FEATURES             Location/Qualifiers
     source          1..13636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    177..198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(213..218,219..236,237..243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    251..267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     256..278
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     275..291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     275..291
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        815..1160
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1103..1123
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1225..1253)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1848..1868
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1951..2203
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2216..2233)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2216..2232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2225..2247)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2372..2391)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2582..2603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    2850..2874
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4470..5618)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(5917..6711)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  6903..7670
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      8293..9008
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(9135..9506)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9539..9648
                     /note="incP origin of transfer"
     primer_bind     9871..9890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10124..10141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(10329..10979)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    11444..11468
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        11592..11771
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        11624..11807
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             11828..12622
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11882..11901)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12492..12511
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      13012..13264
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc
       61 ccttttgtct ttggcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      121 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      181 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      241 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      301 gccaagcttg catgcctgca ggtccccaga ttagcctttt caatttcaga aagaatgcta
      361 acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc tacccgagca
      421 ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
      481 ggactaactg catcaagaac acagagaaag atatatttct caagatcaga agtactattc
      541 cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct
      601 ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc
      661 taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg
      721 acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata
      781 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
      841 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
      901 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
      961 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     1021 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     1081 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     1141 catttcattt ggagagaaca cgggggactc tagaggatcc aaggagatat aacaatgagt
     1201 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1261 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1321 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1381 actttcactt atggtgttca atgcttttca agatacccag atcatatgaa gcggcacgac
     1441 ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga ggaccatctc tttcaaggac
     1501 gacgggaact acaagacacg tgctgaagtc aagtttgagg gagacaccct cgtcaacagg
     1561 atcgagctta agggaatcga tttcaaggag gacggaaaca tcctcggcca caagttggaa
     1621 tacaactaca actcccacaa cgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     1681 gctaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1741 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1801 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1861 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata aggggtaccg
     1921 cggccgcgaa ttcagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     1981 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     2041 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     2101 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     2161 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aattcactgg
     2221 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2281 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2341 cccaacagtt gcgcagcctg aatggcgccc gctcctttcg ctttcttccc ttcctttctc
     2401 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2461 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     2521 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2581 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     2641 ttataaggga ttttgccgat ttcggaacca ccatcaaaca ggattttcgc ctgctggggc
     2701 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     2761 tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa cgtccgcaat
     2821 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     2881 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     2941 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     3001 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     3061 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caaatatcat
     3121 ctccctcgca gagatccgaa ttatcagcct tcttattcat ttctcgctta accgtgacag
     3181 gctgtcgatc ttgagaacta tgccgacata ataggaaatc gctggataaa gccgctgagg
     3241 aagctgagtg gcgctatttc tttagaagtg aacgttgacg atatcaactc ccctatccat
     3301 tgctcaccga atggtacagg tcggggaccc gaagttccga ctgtcggcct gatgcatccc
     3361 cggctgatcg accccagatc tggggctgag aaagcccagt aaggaaacaa ctgtaggttc
     3421 gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag
     3481 ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa
     3541 agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag
     3601 aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc
     3661 cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag
     3721 cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc
     3781 tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc
     3841 gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt
     3901 aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat
     3961 catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc
     4021 tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc
     4081 gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc
     4141 cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta
     4201 aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc
     4261 ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa
     4321 tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag
     4381 caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa
     4441 cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
     4501 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
     4561 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
     4621 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
     4681 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
     4741 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
     4801 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
     4861 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
     4921 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
     4981 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
     5041 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
     5101 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
     5161 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
     5221 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
     5281 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
     5341 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc
     5401 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
     5461 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
     5521 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
     5581 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
     5641 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
     5701 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
     5761 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
     5821 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
     5881 tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat ccagtaaaat
     5941 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
     6001 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
     6061 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
     6121 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
     6181 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
     6241 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
     6301 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
     6361 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
     6421 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
     6481 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
     6541 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
     6601 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
     6661 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
     6721 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     6781 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     6841 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta
     6901 tgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg
     6961 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc
     7021 aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaactg
     7081 cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga tatggccatg
     7141 aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa cacgatttta
     7201 cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg ggcagtgacg
     7261 tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa tcgcgccagc
     7321 gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac gtattcggtg
     7381 aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
     7441 tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
     7501 tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
     7561 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
     7621 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc caattatgat
     7681 agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca tgcaagtttt
     7741 tatgctttgc ccattctata gatatattga taagcgcgct gcctatgcct tgccccctga
     7801 aatccttaca tacggcgata tcttctatat aaaagatata ttatcttatc agtattgtca
     7861 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
     7921 aaatatggcg cttcatagag taattctgta aaggtccaat tctcgttttc atacctcggt
     7981 ataatcttac ctatcacctc aaatggttcg ctgggtttat cgcacccccg aacacgagca
     8041 cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa aattgccggc cccgccatga
     8101 agtccgtgaa tgccccgacg gccgaagtga agggcaggcc gccacccagg ccgccgccct
     8161 cactgcccgg cacctggtcg ctgaatgtcg atgccagcac ctgcggcacg tcaatgcttc
     8221 cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc cccgatcccg gcaatggcaa
     8281 ggactgccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct
     8341 ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc
     8401 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
     8461 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
     8521 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
     8581 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
     8641 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
     8701 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
     8761 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
     8821 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
     8881 caacgccggc ggccggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
     8941 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg
     9001 aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc
     9061 tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt
     9121 agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca
     9181 agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac
     9241 cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg
     9301 tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg
     9361 tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt
     9421 tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc
     9481 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc
     9541 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
     9601 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
     9661 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
     9721 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
     9781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10021 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10081 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10141 ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg aggcttggac
    10201 gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga cgcggtggaa
    10261 agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg ctccggcagc
    10321 ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac gagcctcctt
    10381 ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc cggaccggct
    10441 tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg
    10501 ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc cccaacagtg
    10561 aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag
    10621 aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca
    10681 tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg
    10741 atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc
    10801 agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc
    10861 gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg
    10921 acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg
    10981 cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg ataaagaatc
    11041 cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa cccaacatac
    11101 ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc
    11161 cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg
    11221 gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc
    11281 tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc gccagatctg
    11341 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc
    11401 ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata
    11461 tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga tcatgagcgg
    11521 agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt
    11581 tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc tggagtttaa
    11641 tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact
    11701 atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg
    11761 gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg gatctggatc
    11821 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    11881 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    11941 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    12001 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    12061 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    12121 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    12181 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    12241 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    12301 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
    12361 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    12421 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    12481 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    12541 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    12601 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
    12661 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    12721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    12781 ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat cattacgaca
    12841 gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg gcccggcgtc
    12901 cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg cgatatcttg
    12961 ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc cgatcgttca
    13021 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
    13081 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
    13141 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
    13201 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
    13261 gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg gcggctctga
    13321 gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag gcggttccgg
    13381 tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta ataagggggc
    13441 tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc
    13501 tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt ccggccttgc
    13561 taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg
    13621 tgacggtgat aattca
//
