LOCUS       sequence-238589-        5317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Miao YL, Gambini A, Zhang Y, Padilla-Banks E, Jefferson WN, 
            Bernhardt ML, Huang W, Li L, Williams CJ
  TITLE     Mediator complex component MED13 regulates zygotic genome activation
            and is required for postimplantation development in the mouse.
  JOURNAL   Biol Reprod. 2018 Jan 9. pii: 4794936. doi: 10.1093/biolre/ioy004.
  PUBMED    29325037
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Reprod. 2018 Jan 9. pii: 4794936. doi: 10.1093/biolre/ioy004."
COMMENT     Sequence Label: pF208wt-luc
FEATURES             Location/Qualifiers
     source          1..5317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    54..102
                     /note="synthetic polyadenylation signal"
     misc_feature    116..207
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     156..175
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      1376..1385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1382..3034
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3078..3199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3089..3108)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3143..3162
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3447..3464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3618..4206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3698..3717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4406..5197,5198..5266))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc cgcaataaaa
       61 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat
      121 acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg
      181 caagtgcagg tgccagaaca tttctctggc ctaactggcc ggtacctgag ctcgctagcc
      241 tcgagtaaat actttggatg aaattagacc tggcttttgt catctcaatt tgcctaaatt
      301 tcaacattta aattgtgtaa aagcatctac ttggtatctg ttgtgagaat gtgtctatac
      361 agatggtaac tcagaacccc ttacctagaa agaaactatt gaaaggccct cccttcattt
      421 cactatcaaa ttgttttctt ctaggtggtc gaacattctt ctaggtgtgc gagcattcgt
      481 gcctcccatc attaggttgt aaattgtgcg gaagaatccc aaatattgaa tgtttgctta
      541 tgtagtgctt tggtctggag tacgaccagt gctcgtgatc tactgggaaa gcagaaagtc
      601 tggatggaat aggatgttat caccctgctt atcactggag gccattttct tatctctctt
      661 cactgccttt cttattgttt gtttataatc tttatgcata taggtggctt gcctgcattt
      721 atgatagagc accacacgta tacctagtgc cctccgaggc cagaaaagga cttggattct
      781 ttgaagtgac taactgctgg ttgagttgtc ctatgtcggc tggagctcga attctggtcc
      841 tttggaagat cagctcagag cagaccgatc acacgaccag gatcagccaa ttatgtgccg
      901 ggtttggtcc acagacctcc ggaggaaaga aggtgcttac ttgcaagagt actgtccgaa
      961 agacgtcctc tgggcttgcc tccgtcaaac gcagtgcgga gagggcaggg cccaggaatc
     1021 cgcagaatgc tgccaagccc aagccctcac tctgaagcga tggcgtcgac gctttccccg
     1081 ggcgtggcac tctcctcttt tggcgggctc ggcatcgcga ggctccgccc tcctggaacg
     1141 gtctctctgc agtcgcggag gcgggctctg catttctatt ggtagagcgg gtccgccgag
     1201 gccccgcccc ctcccgggcc cgacgcgagc acgttgggta gccagggccg gttgaaaagc
     1261 cgcccgaatc cggagcagct cccggggcga gaggcacgct tagggccgcg gtggcggtta
     1321 agaggcgtct ctgtggctgg agtgcgaagc ttggcaatcc ggtactgttg gtaaagccac
     1381 catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg
     1441 gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat
     1501 cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag
     1561 cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt
     1621 gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt
     1681 ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat
     1741 cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca
     1801 aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg
     1861 cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga
     1921 cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg
     1981 cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag
     2041 tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt
     2101 ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt
     2161 tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta
     2221 taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct
     2281 catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag
     2341 caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta
     2401 cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg
     2461 cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa
     2521 gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg
     2581 ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag
     2641 cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag
     2701 cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca
     2761 acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct
     2821 gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga
     2881 ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga
     2941 cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat
     3001 taaggccaag aagggcggca agatcgccgt gtaataattc tagagtcggg gcggccggcc
     3061 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3121 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3181 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3241 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     3301 ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     3361 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     3421 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3481 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3541 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4021 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4261 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4321 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
     4381 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
     4441 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
     4501 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
     4561 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
     4621 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
     4681 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
     4741 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
     4801 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
     4861 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
     4921 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
     4981 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
     5041 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
     5101 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
     5161 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
     5221 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
     5281 aatattattg aagcatttat cagggttact agtacgt
//
