LOCUS       sequence-238597-        8514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac transposon plasmid for live animal optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Karlsson J, Tzeng SY, Hemmati S, Luly KM, Choi O, Rui Y, Wilson DR, 
            Kozielski KL, Quinones-Hinojosa A, Green JJ
  TITLE     Photocrosslinked Bioreducible Polymeric Nanoparticles for Enhanced 
            Systemic siRNA Delivery as Cancer Therapy.
  JOURNAL   Adv Funct Mater. 2021 Apr 22;31(17). doi: 10.1002/adfm.202009768. 
            Epub 2021 Feb 22.
  PUBMED    34650390
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Funct 
            Mater."; date: "2021-04-22"; volume: "31(17). doi"; pages: " 
            10.1002/adfm.202009768. Epub 2021 Feb 22"
COMMENT     Sequence Label: PB-fLuc=tdT
FEATURES             Location/Qualifiers
     source          1..8514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   31..93
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    390..423
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          814..1830
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1754..1776
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1838..1857
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1942..3591
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      3640..3649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3646..5076
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(5188..5207)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5253..5308
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5307..5326)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   5979..6013
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6036..6052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6036..6052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6049..6071)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6060..6076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6084..6090,6091..6108,6109..6114))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6129..6150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6209..6404
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6255..6390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6410..6544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6447..6466)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6501..6520
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6612..6629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6783..7371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6863..6882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7542..8333,8334..8402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8165..8184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8403..8507)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccgaaaag tgccacctgg gtcgacttaa ccctagaaag ataatcatat tgtgacgtac
       61 gttaaagata atcatgcgta aaattgacgc atgtgtttta tcgatctgta tatcgaggtt
      121 tatttattaa tttgaataga tattaagttt tattatattt acacttacat actaataata
      181 aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa
      241 tttcttctat aaagtaacaa aacttttaaa cattctctct tttacaaaaa taaacttatt
      301 ttgtacttta aaaacagtca tgttgtatta taaaataagt aattagctta acttatacat
      361 aatagaaaca aattatactt attaatcgca taacttcgta taatgtatgc tatacgaagt
      421 tatgcgatta agggatctgt agggcgcagt agtccaacta gttattaata gtaatcaatt
      481 acggggtcat tagttcatag cccatatatg gagttccgcg tyacatawmy yayggtwmrw
      541 ggysmgccys rckkwcygcy ymackmyccc crtctccccc ccctccccac ccccaatttt
      601 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggncgc
      661 gcgccrggsg gggcggggsg gggsgrgggg sggggsgggg cgaggcggag aggtgcggcg
      721 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      781 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc
      841 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      901 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      961 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct
     1021 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     1081 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1141 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc
     1201 tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg
     1261 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc
     1321 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
     1381 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggrgggctcg
     1441 ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     1501 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     1561 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     1621 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     1681 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
     1741 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1801 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     1861 tctcatcatt ttggcaaaga attctgcagt cgacggtacc gagctcggat ccactagtcc
     1921 agtgtggtgg aattcaccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
     1981 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     2041 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     2101 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     2161 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     2221 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     2281 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     2341 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     2401 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     2461 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     2521 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     2581 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     2641 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     2701 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     2761 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc
     2821 ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc
     2881 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     2941 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     3001 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     3061 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     3121 ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac
     3181 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     3241 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     3301 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     3361 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     3421 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     3481 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     3541 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gggagatcta
     3601 ggaggtggag gtagcggtgg aggtggaagc caggatcccg ccaccatggt gagcaagggc
     3661 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     3721 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     3781 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     3841 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     3901 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     3961 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     4021 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     4081 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     4141 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     4201 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     4261 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     4321 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac
     4381 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     4441 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     4501 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     4561 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     4621 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4681 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     4741 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     4801 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     4861 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     4921 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     4981 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     5041 ctgttcctgt acggcatgga cgagctgtac aagtaagaat tctgcagata tccagcacag
     5101 tggcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5161 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5221 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5281 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5341 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5401 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5461 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5521 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5581 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     5641 cctgcagagc tccagcctac acaatcgctc aagacgtgta atgcttttat tatatattag
     5701 tcacgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     5761 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     5821 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     5881 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
     5941 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     6001 tatctttcta gggttaaaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6061 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6121 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6181 tcgggaaacc tgtcgtgcca gcggatccgc atctcaataa gtcagcaacc atagtcccgc
     6241 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6301 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6361 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     6421 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6481 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6541 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac attt
//
