LOCUS       sequence-238599-         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To transform BL21-Gold(DE3) and be induced by IPTG for 
            His-proteinG-MNase fusion protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  TITLE     To transform BL21-Gold(DE3) and be induced by IPTG for 
            His-proteinG-MNase fusion protein expression.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15bHis6-pG-MNase
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctggtgccgc gcggcagcca tttgaagggt gaaacgacca cagaagcagt agacgcggcc
       61 actgccgaga aggtgtttaa acaatatgcg aacgacaacg gagtcgatgg ggaatggact
      121 tatgacgatg caaccaaaac tttcactgtt acggagaagc ccgaagtaat tgacgccagc
      181 gaacttaccc ccgcggtaac gacgtataaa cttgtaatta atggtaagac gctgaagggg
      241 gagaccacca ctgaggccgt agatgcagct acagccgaga aggtctttaa acagtatgcc
      301 aatgataatg gtgtcgacgg tgagtggacg tacgatgatg caactaagac attcaccgta
      361 acagaaaagc cggaagttat tgacgcgtcg gaacttactc ctgcggtgac cacttacaaa
      421 ctggtaatca acggcaagac gcttaaggga gaaacaacga ctaaagccgt cgacgccgaa
      481 acggctgaga aagctttcaa gcaatacgcc aatgataatg gggtagacgg ggtttggacg
      541 tatgatgacg ccaccaagac ttttacagtc acggaaatgg gtggtggaat gataac
//
