LOCUS       sequence-238609-        1515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For genome editing in plant by SpCas9 variant(SpCas9-NG) 
            (D10A)-PmCDA1-UGI base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pGEL066
FEATURES             Location/Qualifiers
     source          1..1515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             208..273
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             907..927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             934..1185
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             1186..1239
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1426..1446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1459..1479
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1492..1512
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggaggtggcg gtacaggagg gggtgggtcc gctgagtatg tcagggcgtt gttcgacttc
       61 aatggaaacg acgaggaaga tctgcctttt aaaaagggag atattctcag gatcagagat
      121 aagccggaag aacaatggtg gaacgctgaa gactctgaag gtaagagagg tatgattctt
      181 gtcccctacg tcgagaagta ttcgggtgac tataaagacc acgatggaga ttataaggac
      241 cacgatatag attataagga tgatgatgat aagagcggaa tgaccgatgc agagtacgtc
      301 aggattcatg agaaacttga catctacacg tttaagaaac agtttttcaa caacaaaaaa
      361 tctgttagtc accgctgtta cgtgctgttc gaattgaaac gcagaggtga gaggagagcc
      421 tgcttttggg gctatgccgt caacaagccg caaagcggca cagaaagggg cattcacgcg
      481 gagatattta gcattagaaa ggtcgaggaa taccttcggg ataatcccgg gcaattcact
      541 atcaattggt actcttcatg gtccccgtgt gcagattgcg ctgaaaagat actggagtgg
      601 tataatcaag aactcagagg aaacggtcac accctcaaga tttgggcttg caagctttac
      661 tacgagaaaa atgcaaggaa ccagatcggc ctctggaact tgcgcgacaa cggcgtgggg
      721 ttgaatgtga tggtgtcgga gcattaccag tgctgccgga agatattcat tcagtcgtca
      781 cataatcaat tgaacgagaa taggtggctc gaaaaaaccc tgaagcgggc cgagaagtgg
      841 aggagtgaac tctcgataat gatccaggtt aaaatactgc atactaccaa atctccggcg
      901 gtgggaccga agaagaagcg caaggtgggg accatgacta atctctcaga tataatcgag
      961 aaggaaacag gaaagcaact ggtcatccaa gaatcgattt tgatgcttcc cgaagaagtc
     1021 gaagaagtta taggaaataa gcccgagtct gacatactgg ttcacacagc gtacgatgaa
     1081 agtacggacg agaatgtcat gttgctgaca tcggacgcac ctgaatacaa gccttgggct
     1141 ctggtcatac aagatagtaa cggagaaaat aagattaaaa tgcttgaagg aaggggctcc
     1201 ctgctgacat gtggagatgt ggaagagaat ccaggaccat ccaggatgat agagcaagat
     1261 ggttgtaccc aagtgctgcg gcccctcgga tggcgggggt acagcgccat gactggccat
     1321 aatagacagt ccgctgctct catgcctcca tgcagcggat gtcagaggag gggagctagg
     1381 ttcttcctgt ctagacctac ctgccccgtg ccctcaaggg ctgatcctaa gaagaagagg
     1441 aaggtttcta gggcagatcc aaagaagaag cggaaggtgt ctagagctga tccgaagaag
     1501 aagcgcaagg tgtga
//
