LOCUS       sequence-238610-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 
            localization in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Lerner AM, Hepperla AJ, Keele GR, Meriesh HA, Yumerefendi H, 
            Restrepo D, Zimmerman S, Bear JE, Kuhlman B, Davis IJ, Strahl BD
  TITLE     An optogenetic switch for the Set2 methyltransferase provides 
            evidence for transcription-dependent and -independent dynamics of 
            H3K36 methylation.
  JOURNAL   Genome Res. 2020 Nov;30(11):1605-1617. doi: 10.1101/gr.264283.120. 
            Epub 2020 Oct 5.
  PUBMED    33020206
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.264283.120"; journalName: "Genome Res"; date: "2020-11";
            volume: "30"; issue: "11"; pages: "1605-1617"
COMMENT     Sequence Label: pNIA-CEN-FLAG-LANS1-Set2
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(133..138,139..156,157..163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     176..198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     195..211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     230..250
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        232..250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1019..1723
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1777..1800
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4552..4799
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4568..4586)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4817..4836)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4818..4836)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4846..4863)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4846..4862)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4855..4877)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4998..5015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5169..5757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5249..5268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5928..6719,6720..6788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6551..6570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6789..6893)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6961..6979
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6999..7404
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7405..8499
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    8861..10023
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      8861..9698
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc acgacaggtt
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
      241 tcactaaagg gaacaaaagc tggagctcgc cgggatcgaa gaaatgatgg taaatgaaat
      301 aggaaatcaa ggagcatgaa ggcaaaagac aaatataagg gtcgaacgaa aaataaagtg
      361 aaaagtgttg atatgatgta tttggctttg cggcgccgaa aaaacgagtt tacgcaattg
      421 cacaatcatg ctgactctgt ggcggacccg cgctcttgcc ggcccggcga taacgctggg
      481 cgtgaggctg tgcccggcgg agttttttgc gcctgcattt tccaaggttt accctgcgct
      541 aaggggcgag attggagaag caataagaat gccggttggg gttgcgatga tgacgaccac
      601 gacaactggt gtcattattt aagttgccga aagaacctga gtgcatttgc aacatgagta
      661 tactagaaga atgagccaag acttgcgaga cgcgagtttg ccggtggtgc gaacaataga
      721 gcgaccatga ccttgaaggt gagacgcgca taaccgctag agtactttga agaggaaaca
      781 gcaatagggt tgctaccagt ataaatagac aggtacatac aacactggaa atggttgtct
      841 gtttgagtac gctttcaatt catttgggtg tgcactttat tatgttacaa tatggaaggg
      901 aactttacac ttctcctatg cacatatatt aattaaagtc caatgctagt agagaagggg
      961 ggtaacaccc ctccgcgctc ttttccgatt tttttctaaa ccgtggaata tttcggatat
     1021 ccttttgttg tttccgggtg tacaatatgg acttcctctt ttctggcaac caaacccata
     1081 catcgggatt cctataatac cttcgttggt ctccctaaca tgtaggtggc ggaggggaga
     1141 tatacaatag aacagatacc agacaagaca taatgggcta aacaagacta caccaattac
     1201 actgcctcat tgatggtggt acataacgaa ctaatactgt agccctagac ttgatagcca
     1261 tcatcatatc gaagtttcac tacccttttt ccatttgcca tctattgaag taataatagg
     1321 cgcatgcaac ttcttttctt tttttttctt ttctctctcc cccgttgttg tctcaccata
     1381 tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa aaattaacga caaagacagc
     1441 accaacagat gtcgttgttc cagagctgat gaggggtatc tcgaagcaca cgaaactttt
     1501 tccttccttc attcacgcac actactctct aatgagcaac ggtatacggc cttccttcca
     1561 gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc tatcaagtat aaatagacct
     1621 gcaattatta atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt
     1681 tttctgcaca atatttcaag ctataccaag catacaatca actccaagct tgaattaatt
     1741 ccgggcggaa tgaaagcgtt aacgtctggc ggccgcgatt acaaggacga cgatgacaag
     1801 tctagactgg cgctgaaact ggcgggcctg gatattggcc tgcagggttc tggaagtggg
     1861 tcgttggcta ctacacttga acgtattgag aagaactttg tcattactga cccaagattg
     1921 ccagataatc ccattatatt cgcgtccgat agtttcttgc agttgacaga atatagccgt
     1981 gaagaaattt tgggaagaaa ctgcaggttt ctacaaggtc ctgaaactga tcgcgcgaca
     2041 gtgagaaaaa ttagagatgc catagataac caaacagagg tcactgttca gctgattaat
     2101 tatacaaaga gtggtaaaaa gttctggaac ctctttcact tgcagcctat gcgagatcag
     2161 aagggagatg tccagtactt tattggggtt cagttggatg gaactgagca tgtccgagat
     2221 gctgccgaga gagagggagt catgctgatt aagaaaactg cagaaaatat tgatatggcg
     2281 gcaaaaagag tcaaactgga tgggtctgga agtgggtcgg ggtcccgggg aatgtcgaag
     2341 aaccaaagtg tgagtgcgtc ggaagatgaa aaagaaatac tgaataacaa cgctgagggc
     2401 cataaacctc aaaggctttt tgatcaagag cctgatctca cagaggaagc attgaccaaa
     2461 tttgaaaact tggatgactg tatatatgcg aacaagagga tcggaacatt taagaataat
     2521 gatttcatgg aatgtgactg ttatgaagaa ttctcagatg gcgtcaacca tgcctgtgac
     2581 gaggattctg actgtatcaa tagacttacg ttgatagaat gtgtcaacga tttgtgttca
     2641 tcttgtggta atgattgtca aaatcagaga tttcaaaaga agcagtacgc tccgatagcc
     2701 atatttaaaa caaagcataa aggttatggc gtaagagccg aacaggacat agaagccaac
     2761 cagttcattt acgagtacaa aggtgaggtg atcgaggaga tggaatttag agataggtta
     2821 atagactatg accaacgtca tttcaagcat ttttatttta tgatgttaca gaacggagaa
     2881 ttcattgacg ccacaataaa gggttcgttg gccagatttt gcaatcactc ttgcagcccc
     2941 aatgcatatg ttaataaatg ggttgttaag gataagctac gcatgggaat atttgctcaa
     3001 agaaaaattt taaaaggtga ggaaatcact tttgattata atgtggatcg ttatggtgct
     3061 caagctcaga aatgctactg tgaggagcca aattgtattg ggtttctcgg tggtaagact
     3121 caaacagatg cggcatcttt attgccccag aacatcgctg atgccttggg agtgactgtt
     3181 tccatggaga aaaaatggct aaagttaaag aaattaagcg gtgagccaat aataaagaat
     3241 gaaaacgaga acataaatat tgaatttctt caatcattgg aagtccaacc tatcgacagc
     3301 ccagtggatg tgacgaagat catgagtgta ttactacagc aagataataa gataattgca
     3361 tccaaactct tgaagagact ttttactatc gatgatgact ctcttcgtca tcaggctatc
     3421 aaactacacg gttacacctg ttttagcaaa atgcttaaat tgtttatcac ggaacaacct
     3481 caggtagatg gaaaaggaaa cgagactgaa gaagatgata taaagtttat aaagggaatt
     3541 ttagattttt tgttggaatt accaaagaca actcgaaacg gtattgagtc gtcacaaatt
     3601 gataatgtag taaaaactct ccctgcaaag tttccatttt taaagccaaa ctgcgatgag
     3661 ttattggaga aatggtcgaa atttgaaaca tataaaagga taacgaagaa agacataaac
     3721 gtcgcagcca gcaaaatgat agatttgaga agggtgagat tacctccagg ctgggagatc
     3781 attcacgaaa acggtcgccc attgtattat aatgcagaac agaaaacgaa actacactat
     3841 ccaccttcgg gatcatctaa ggtctttagt tcaaggtcaa atacacaagt aaattctccg
     3901 agtagcagtg gaataccaaa aacgcctggc gctttagact ctggtagtca taagctgagt
     3961 gacgaagaat atgagggtag tggtcaaaaa agactggaat acgaaagaat tgcgctagaa
     4021 agagccaaac aagaagaatt ggaaagcctg aagcaaaagc taaagcttga gaatgaaaga
     4081 aaaagcgttt tggaggatat tatagcggag gccaacaaac agaaggagtt acaaaaggaa
     4141 gaggccaaaa aactagtgga agcaaaagag gctaagcggt tgaaaagaaa aacggtctcc
     4201 cagtcccaaa gactagagca caactggaat aaattctttg catcttttgt acccaactta
     4261 ataaaaaaaa acccacaaag taaacaattt gatcatgaaa acattaaaca atgtgctaaa
     4321 gacatcgtga aaatcctaac tacaaaggaa cttaaaaaag attcttcaag agcgccgcct
     4381 gatgacttaa ccaaaggaaa aaggcataaa gttaaagaat tcataaattc atacatggat
     4441 aagattatcc tcaagaagaa gcaaaaaaag gcgttggcat tatcatcagc atcaacaagg
     4501 atgtcttctc ctccaccttc aacatcatca taaggatccg tcgacctcga gtcatgtaat
     4561 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
     4621 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
     4681 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
     4741 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg
     4801 gccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     4861 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4921 ccctttcgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4981 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5041 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5101 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5161 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5221 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5281 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5341 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5401 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5461 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5521 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5581 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5641 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5701 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5761 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5821 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5881 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5941 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6001 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6061 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6121 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6181 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6241 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6301 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6361 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6421 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6481 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6541 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6601 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6661 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6721 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6781 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6841 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6901 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     6961 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ctgtgggaat actcaggtat
     7021 cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca acataacgag
     7081 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
     7141 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
     7201 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
     7261 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
     7321 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
     7381 atatttcaag gatataccat tctaatgtct gcccctaaga agatcgtcgt tttgccaggt
     7441 gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc tatttctgat
     7501 gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat
     7561 gctacaggtg ttccacttcc agatgaggcg ctggaagcct ccaagaaggc tgatgccgtt
     7621 ttgttaggtg ctgtgggtgg tcctaaatgg ggtacaggta gtgttagacc tgaacaaggt
     7681 ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg taactttgca
     7741 tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg tactgacttc
     7801 gttgttgtca gagaattagt gggaggtatt tactttggta agagaaagga agacgatggt
     7861 gatggtgtcg cttgggatag tgaacaatac accgttccag aagtgcaaag aatcacaaga
     7921 atggccgctt tcatggccct acaacatgag ccaccattgc ctatttggtc cttggataaa
     7981 gctaatgttt tggcctcttc aagattatgg agaaaaactg tggaggaaac catcaagaac
     8041 gaatttccta cattgaaggt tcaacatcaa ttgattgatt ctgccgccat gatcctagtt
     8101 aagaacccaa cccacctaaa tggtattata atcaccagca acatgtttgg tgatatcatc
     8161 tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc gtccttggcc
     8221 tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccacgg ttctgctcca
     8281 gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc aatgatgttg
     8341 aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt taaaaaggtt
     8401 ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac cgaagtcggt
     8461 gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct ttttttatga
     8521 tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa attacaaaat
     8581 ggaatatgtt catagggtag aattaattct catgtttgac agcttatcat cgatcgtcca
     8641 actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa
     8701 aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca
     8761 ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa
     8821 ctcgatttct gactgggttg gaaggcaaga gagccccgag agcttacatt ttatgttagc
     8881 tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt
     8941 tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt
     9001 cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg
     9061 cacttgcctg caagcctttt gaaaagcaag cataaaagat ctaaacataa aatctgtaaa
     9121 ataacaagat gtaaagataa tgctaaatca tttggctttt tgattgattg tacaggaaaa
     9181 tatacatcgc agggggttga cttttaccat ttcaccgcaa tggaatcaaa cttgttgaag
     9241 agaatgttca caggcgcata cgctacaatg acccgattct tgctagcctt ttctcggtct
     9301 tgcaaacaac cgccggcagc ttagtatata aatacacatg tacatacctc tctccgtatc
     9361 ctcgtaatca ttttcttgta tttatcgtct tttcgctgta aaaactttat cacacttatc
     9421 tcaaatacac ttattaaccg cttttactat tatcttctac gctgacagta atatcaaaca
     9481 gtgacacata ttaaacacag tggtttcttt gcataaacac catcagcctc aagtcgtcaa
     9541 gtaaagattt cgtgttcatg cagatagata acaatctata tgttgataat tagcgttgcc
     9601 tcatcaatgc gagatccgtt taaccggacc ctagtgcact taccccacgt tcggtccact
     9661 gtgtgccgaa catgctcctt cactatttta acatgtggaa ttaattctca tgtttgacag
     9721 cttatcatcg aactctaaga ggtgatactt atttactgta aaactgtgac gataaaaccg
     9781 gaaggaagaa taagaaaact cgaactgatc tataatgcct attttctgta aagagtttaa
     9841 gctatgaaag cctcggcatt ttggccgctc ctaggtagtg ctttttttcc aaggacaaaa
     9901 cagtttcttt ttcttgagca ggttttatgt ttcggtaatc ataaacaata aataaattat
     9961 ttcatttatg tttaaaaata aaaaataaaa aagtatttta aatttttaaa aaagttgatt
    10021 ataagcatgt gaccttttgc aagcaattaa attttgcaat ttgtgattta ggcaaaagtt
    10081 actatttctg gctcgtgtaa tatatgtatg ctaatgtgaa cttttacaaa gtcgatatgg
    10141 acttagtcaa aagaaatttt cttaaaaata tatagcacta gccaatttag cacttcttta
    10201 tgagatatat tatagacttt attaagccag atttgtgtat tatatgtatt tacccggcga
    10261 atcatggaca tacattctga aataggtaat attctctatg gtgagacagc atagataacc
    10321 taggatacaa gttaaaagct agtactgttt tgcagtaatt tttttctttt ttataagaat
    10381 gttaccacct aaataagtta taaagtcaat agttaagttt gatatttgat tgtaaaatac
    10441 cgtaatatat ttgcatgatc aaaaggctca atgttgacta gccagcatgt caaccactat
    10501 attgatcacc gatattagga cttccacacc aactagtaat atgacaataa attcaagata
    10561 ttcttcatga gaatggccca gcgattatgc ggtgtgaaat accgcacaga tgcgtaagga
    10621 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caac
//
