LOCUS       sequence-238611-       11387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light 
            control of Set2 localization in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Lerner AM, Hepperla AJ, Keele GR, Meriesh HA, Yumerefendi H, 
            Restrepo D, Zimmerman S, Bear JE, Kuhlman B, Davis IJ, Strahl BD
  TITLE     An optogenetic switch for the Set2 methyltransferase provides 
            evidence for transcription-dependent and -independent dynamics of 
            H3K36 methylation.
  JOURNAL   Genome Res. 2020 Nov;30(11):1605-1617. doi: 10.1101/gr.264283.120. 
            Epub 2020 Oct 5.
  PUBMED    33020206
REFERENCE   2  (bases 1 to 11387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.264283.120"; journalName: "Genome Res"; date: "2020-11";
            volume: "30"; issue: "11"; pages: "1605-1617"
COMMENT     Sequence Label: pNIA-CEN-mVenus-FLAG-LANS1-Set2
FEATURES             Location/Qualifiers
     source          1..11387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    236..257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(272..277,278..295,296..302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    310..326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     315..337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     334..350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     334..350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     369..389
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        371..389
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1158..1862
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             join(1904..1906,1907..2617)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1946..1967)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2207..2226)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2554..2575
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2639..2662
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      5414..5661
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5430..5448)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5679..5698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5680..5698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5708..5725)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5708..5724)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5717..5739)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5860..5877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6031..6619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6111..6130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6790..7581,7582..7650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7413..7432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7651..7755)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7823..7841
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7861..8266
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8267..9361
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    9723..10885
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      9723..10560
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 acaccaacta gtaatatgac aataaattca agatattctt catgagaatg gcccagcgat
       61 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      121 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      181 ccagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      361 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctcgcc gggatcgaag
      421 aaatgatggt aaatgaaata ggaaatcaag gagcatgaag gcaaaagaca aatataaggg
      481 tcgaacgaaa aataaagtga aaagtgttga tatgatgtat ttggctttgc ggcgccgaaa
      541 aaacgagttt acgcaattgc acaatcatgc tgactctgtg gcggacccgc gctcttgccg
      601 gcccggcgat aacgctgggc gtgaggctgt gcccggcgga gttttttgcg cctgcatttt
      661 ccaaggttta ccctgcgcta aggggcgaga ttggagaagc aataagaatg ccggttgggg
      721 ttgcgatgat gacgaccacg acaactggtg tcattattta agttgccgaa agaacctgag
      781 tgcatttgca acatgagtat actagaagaa tgagccaaga cttgcgagac gcgagtttgc
      841 cggtggtgcg aacaatagag cgaccatgac cttgaaggtg agacgcgcat aaccgctaga
      901 gtactttgaa gaggaaacag caatagggtt gctaccagta taaatagaca ggtacataca
      961 acactggaaa tggttgtctg tttgagtacg ctttcaattc atttgggtgt gcactttatt
     1021 atgttacaat atggaaggga actttacact tctcctatgc acatatatta attaaagtcc
     1081 aatgctagta gagaaggggg gtaacacccc tccgcgctct tttccgattt ttttctaaac
     1141 cgtggaatat ttcggatatc cttttgttgt ttccgggtgt acaatatgga cttcctcttt
     1201 tctggcaacc aaacccatac atcgggattc ctataatacc ttcgttggtc tccctaacat
     1261 gtaggtggcg gaggggagat atacaataga acagatacca gacaagacat aatgggctaa
     1321 acaagactac accaattaca ctgcctcatt gatggtggta cataacgaac taatactgta
     1381 gccctagact tgatagccat catcatatcg aagtttcact accctttttc catttgccat
     1441 ctattgaagt aataataggc gcatgcaact tcttttcttt ttttttcttt tctctctccc
     1501 ccgttgttgt ctcaccatat ccgcaatgac aaaaaaatga tggaagacac taaaggaaaa
     1561 aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg aggggtatct
     1621 cgaagcacac gaaacttttt ccttccttca ttcacgcaca ctactctcta atgagcaacg
     1681 gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaaagtttg ctgtcttgct
     1741 atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt gttctcgttc
     1801 cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc atacaatcaa
     1861 ctccaagctt gaattaattc cgggcggaat gaaagcgtta acggtgagca agggcgagga
     1921 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1981 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2041 gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta
     2101 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2161 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2221 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2281 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2341 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg caaacttcaa
     2401 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     2461 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa
     2521 actgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2581 cgccgggatc actctcggca tggacgagct gtacaagggt tctggatctg gcggccgcga
     2641 ttacaaggac gacgatgaca agtctagact ggcgctgaaa ctggcgggcc tggatattgg
     2701 cctgcagggt tctggaagtg ggtcgttggc tactacactt gaacgtattg agaagaactt
     2761 tgtcattact gacccaagat tgccagataa tcccattata ttcgcgtccg atagtttctt
     2821 gcagttgaca gaatatagcc gtgaagaaat tttgggaaga aactgcaggt ttctacaagg
     2881 tcctgaaact gatcgcgcga cagtgagaaa aattagagat gccatagata accaaacaga
     2941 ggtcactgtt cagctgatta attatacaaa gagtggtaaa aagttctgga acctctttca
     3001 cttgcagcct atgcgagatc agaagggaga tgtccagtac tttattgggg ttcagttgga
     3061 tggaactgag catgtccgag atgctgccga gagagaggga gtcatgctga ttaagaaaac
     3121 tgcagaaaat attgatatgg cggcaaaaag agtcaaactg gatgggtctg gaagtgggtc
     3181 ggggtcccgg ggaatgtcga agaaccaaag tgtgagtgcg tcggaagatg aaaaagaaat
     3241 actgaataac aacgctgagg gccataaacc tcaaaggctt tttgatcaag agcctgatct
     3301 cacagaggaa gcattgacca aatttgaaaa cttggatgac tgtatatatg cgaacaagag
     3361 gatcggaaca tttaagaata atgatttcat ggaatgtgac tgttatgaag aattctcaga
     3421 tggcgtcaac catgcctgtg acgaggattc tgactgtatc aatagactta cgttgataga
     3481 atgtgtcaac gatttgtgtt catcttgtgg taatgattgt caaaatcaga gatttcaaaa
     3541 gaagcagtac gctccgatag ccatatttaa aacaaagcat aaaggttatg gcgtaagagc
     3601 cgaacaggac atagaagcca accagttcat ttacgagtac aaaggtgagg tgatcgagga
     3661 gatggaattt agagataggt taatagacta tgaccaacgt catttcaagc atttttattt
     3721 tatgatgtta cagaacggag aattcattga cgccacaata aagggttcgt tggccagatt
     3781 ttgcaatcac tcttgcagcc ccaatgcata tgttaataaa tgggttgtta aggataagct
     3841 acgcatggga atatttgctc aaagaaaaat tttaaaaggt gaggaaatca cttttgatta
     3901 taatgtggat cgttatggtg ctcaagctca gaaatgctac tgtgaggagc caaattgtat
     3961 tgggtttctc ggtggtaaga ctcaaacaga tgcggcatct ttattgcccc agaacatcgc
     4021 tgatgccttg ggagtgactg tttccatgga gaaaaaatgg ctaaagttaa agaaattaag
     4081 cggtgagcca ataataaaga atgaaaacga gaacataaat attgaatttc ttcaatcatt
     4141 ggaagtccaa cctatcgaca gcccagtgga tgtgacgaag atcatgagtg tattactaca
     4201 gcaagataat aagataattg catccaaact cttgaagaga ctttttacta tcgatgatga
     4261 ctctcttcgt catcaggcta tcaaactaca cggttacacc tgttttagca aaatgcttaa
     4321 attgtttatc acggaacaac ctcaggtaga tggaaaagga aacgagactg aagaagatga
     4381 tataaagttt ataaagggaa ttttagattt tttgttggaa ttaccaaaga caactcgaaa
     4441 cggtattgag tcgtcacaaa ttgataatgt agtaaaaact ctccctgcaa agtttccatt
     4501 tttaaagcca aactgcgatg agttattgga gaaatggtcg aaatttgaaa catataaaag
     4561 gataacgaag aaagacataa acgtcgcagc cagcaaaatg atagatttga gaagggtgag
     4621 attacctcca ggctgggaga tcattcacga aaacggtcgc ccattgtatt ataatgcaga
     4681 acagaaaacg aaactacact atccaccttc gggatcatct aaggtcttta gttcaaggtc
     4741 aaatacacaa gtaaattctc cgagtagcag tggaatacca aaaacgcctg gcgctttaga
     4801 ctctggtagt cataagctga gtgacgaaga atatgagggt agtggtcaaa aaagactgga
     4861 atacgaaaga attgcgctag aaagagccaa acaagaagaa ttggaaagcc tgaagcaaaa
     4921 gctaaagctt gagaatgaaa gaaaaagcgt tttggaggat attatagcgg aggccaacaa
     4981 acagaaggag ttacaaaagg aagaggccaa aaaactagtg gaagcaaaag aggctaagcg
     5041 gttgaaaaga aaaacggtct cccagtccca aagactagag cacaactgga ataaattctt
     5101 tgcatctttt gtacccaact taataaaaaa aaacccacaa agtaaacaat ttgatcatga
     5161 aaacattaaa caatgtgcta aagacatcgt gaaaatccta actacaaagg aacttaaaaa
     5221 agattcttca agagcgccgc ctgatgactt aaccaaagga aaaaggcata aagttaaaga
     5281 attcataaat tcatacatgg ataagattat cctcaagaag aagcaaaaaa aggcgttggc
     5341 attatcatca gcatcaacaa ggatgtcttc tcctccacct tcaacatcat cataaggatc
     5401 cgtcgacctc gagtcatgta attagttatg tcacgcttac attcacgccc tccccccaca
     5461 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     5521 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     5581 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     5641 cgctcgaagg ctttaatttg cggccggtac ccaattcgcc ctatagtgag tcgtattacg
     5701 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     5761 ttaatcgcct tgcagcacat ccccctttcg ccagctgcat taatgaatcg gccaacgcgc
     5821 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5881 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5941 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6541 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6601 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6661 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6721 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6781 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6841 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6901 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6961 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7021 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7081 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7141 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7201 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7261 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7321 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7381 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7441 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7501 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7561 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7621 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7681 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7741 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     7801 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     7861 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta
     7921 tttttttcct caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat
     7981 gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa
     8041 aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga
     8101 agcgttcatg actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac
     8161 ctattgtttt ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt
     8221 tacatttcag caatatatat atatatttca aggatatacc attctaatgt ctgcccctaa
     8281 gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa
     8341 ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt
     8401 aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg cgctggaagc
     8461 ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtacagg
     8521 tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa
     8581 cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca
     8641 atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg
     8701 taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc
     8761 agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt
     8821 gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac
     8881 tgtggaggaa accatcaaga acgaatttcc tacattgaag gttcaacatc aattgattga
     8941 ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag
     9001 caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt
     9061 gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga
     9121 accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta tcgccactat
     9181 cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat
     9241 tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc
     9301 caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta
     9361 aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag
     9421 aaacgacacg aaattacaaa atggaatatg ttcatagggt agaattaatt ctcatgtttg
     9481 acagcttatc atcgatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt
     9541 tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca
     9601 gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga
     9661 caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg
     9721 agagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta
     9781 gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag
     9841 actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt
     9901 atttatttaa gtattgtttg tgcacttgcc tgcaagcctt ttgaaaagca agcataaaag
     9961 atctaaacat aaaatctgta aaataacaag atgtaaagat aatgctaaat catttggctt
    10021 tttgattgat tgtacaggaa aatatacatc gcagggggtt gacttttacc atttcaccgc
    10081 aatggaatca aacttgttga agagaatgtt cacaggcgca tacgctacaa tgacccgatt
    10141 cttgctagcc ttttctcggt cttgcaaaca accgccggca gcttagtata taaatacaca
    10201 tgtacatacc tctctccgta tcctcgtaat cattttcttg tatttatcgt cttttcgctg
    10261 taaaaacttt atcacactta tctcaaatac acttattaac cgcttttact attatcttct
    10321 acgctgacag taatatcaaa cagtgacaca tattaaacac agtggtttct ttgcataaac
    10381 accatcagcc tcaagtcgtc aagtaaagat ttcgtgttca tgcagataga taacaatcta
    10441 tatgttgata attagcgttg cctcatcaat gcgagatccg tttaaccgga ccctagtgca
    10501 cttaccccac gttcggtcca ctgtgtgccg aacatgctcc ttcactattt taacatgtgg
    10561 aattaattct catgtttgac agcttatcat cgaactctaa gaggtgatac ttatttactg
    10621 taaaactgtg acgataaaac cggaaggaag aataagaaaa ctcgaactga tctataatgc
    10681 ctattttctg taaagagttt aagctatgaa agcctcggca ttttggccgc tcctaggtag
    10741 tgcttttttt ccaaggacaa aacagtttct ttttcttgag caggttttat gtttcggtaa
    10801 tcataaacaa taaataaatt atttcattta tgtttaaaaa taaaaaataa aaaagtattt
    10861 taaattttta aaaaagttga ttataagcat gtgacctttt gcaagcaatt aaattttgca
    10921 atttgtgatt taggcaaaag ttactatttc tggctcgtgt aatatatgta tgctaatgtg
    10981 aacttttaca aagtcgatat ggacttagtc aaaagaaatt ttcttaaaaa tatatagcac
    11041 tagccaattt agcacttctt tatgagatat attatagact ttattaagcc agatttgtgt
    11101 attatatgta tttacccggc gaatcatgga catacattct gaaataggta atattctcta
    11161 tggtgagaca gcatagataa cctaggatac aagttaaaag ctagtactgt tttgcagtaa
    11221 tttttttctt ttttataaga atgttaccac ctaaataagt tataaagtca atagttaagt
    11281 ttgatatttg attgtaaaat accgtaatat atttgcatga tcaaaaggct caatgttgac
    11341 tagccagcat gtcaaccact atattgatca ccgatattag gacttcc
//
