LOCUS       sequence-238638-        8627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2 ICE plasmid used to facilitate yeast recombination cloning and 
            stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2
FEATURES             Location/Qualifiers
     source          1..8627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..276
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             277..4455
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(4856..4875)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4975..4997
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5035..5053)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5121..5225
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5226..5294,5295..6086)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5444..5463)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6257..6845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6746..6765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6999..7016
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7133..7154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7169..7174,7175..7192,7193..7199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7207..7223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7212..7234
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7231..7247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7231..7247
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7266..7286
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7268..7286
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7341..7844
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7875..7891
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7875..7891
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7925..7941)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7926..7942)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7966..7985)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7967..7985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7995..8012)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7995..8011)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8004..8026)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8152..8607
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8239..8258)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8449..8470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttacgcatct gtgcggtatt tcacaccgca tagatcaatt ccacttgcaa ttacataaaa
       61 aattccggcg gtttttcgcg tgtgactcaa tgtcgaaata cctgcctaat gaacatgaac
      121 atcgcccaaa tgtatttgaa gacccgctgg gagaagttca agatatataa gtaacaagca
      181 gccaatagta taaaaaaaaa tctgagttta ttacctttcc tggaatttca gtgaaaaact
      241 gctaattata gagagatatc acagagttac tcactaatga ctaacgaaaa ggtctggata
      301 gagaagttgg ataatccaac tctttcagtg ttaccacatg actttttacg cccacaacaa
      361 gaaccttata cgaaacaagc tacatattcg ttacagctac ctcagctcga tgtgcctcat
      421 gatagttttt ctaacaaata cgctgtcgct ttgagtgtat gggctgcatt gatatataga
      481 gtaaccggtg acgatgatat tgttctttat attgcgaata acaaaatctt aagattcaat
      541 attcaaccaa cgtggtcatt taatgagctg tattctacaa ttaacaatga gttgaacaag
      601 ctcaattcta ttgaggccaa tttttccttt gacgagctag ctgaaaaaat tcaaagttgc
      661 caagatctgg aaaggacccc tcagttgttc cgtttggcct ttttggaaaa ccaagatttc
      721 aaattagacg agttcaagca tcatttagtg gactttgctt tgaatttgga taccagtaat
      781 aatgcgcatg ttttgaactt aatttataac agcttactgt attcgaatga aagagtaacc
      841 attgttgcgg accaatttac tcaatatttg actgctgcgc taagcgatcc atccaattgc
      901 ataactaaaa tctctctgat caccgcatca tccaaggata gtttacctga tccaactaag
      961 aacttgggct ggtgcgattt cgtggggtgt attcacgaca ttttccagga caatgctgaa
     1021 gccttcccag agagaacctg tgttgtggag actccaacac taaattccga caagtcccgt
     1081 tctttcactt atcgcgacat caaccgcact tctaacatag ttgcccatta tttgattaaa
     1141 acaggtatca aaagaggtga tgtagtgatg atctattctt ctaggggtgt ggatttgatg
     1201 gtatgtgtga tgggtgtctt gaaagccggc gcaacctttt cagttatcga ccctgcatat
     1261 cccccagcca gacaaaccat ttacttaggt gttgctaaac cacgtgggtt gattgttatt
     1321 agagctgctg gacaattgga tcaactagta gaagattaca tcaatgatga attggagatt
     1381 gtttcaagaa tcaattccat cgctattcaa gaaaatggta ccattgaagg tggcaaattg
     1441 gacaatggcg aggatgtttt ggctccatat gatcactaca aagacaccag aacaggtgtt
     1501 gtagttggac cagattccaa cccaacccta tctttcacat ctggttccga aggtattcct
     1561 aagggtgttc ttggtagaca tttttccttg gcttattatt tcaattggat gtccaaaagg
     1621 ttcaacttaa cagaaaatga taaattcaca atgctgagcg gtattgcaca tgatccaatt
     1681 caaagagata tgtttacacc attattttta ggtgcccaat tgtatgtccc tactcaagat
     1741 gatattggta caccgggccg tttagcggaa tggatgagta agtatggttg cacagttacc
     1801 catttaacac ctgccatggg tcaattactt actgcccaag ctactacacc attccctaag
     1861 ttacatcatg cgttctttgt gggtgacatt ttaacaaaac gtgattgtct gaggttacaa
     1921 accttggcag aaaattgccg tattgttaat atgtacggta ccactgaaac acagcgtgca
     1981 gtttcttatt tcgaagttaa atcaaaaaat gacgatccaa actttttgaa aaaattgaaa
     2041 gatgtcatgc ctgctggtaa aggtatgttg aacgttcagc tactagttgt taacaggaac
     2101 gatcgtactc aaatatgtgg tattggcgaa ataggtgaga tttatgttcg tgcaggtggt
     2161 ttggccgaag gttatagagg attaccagaa ttgaataaag aaaaatttgt gaacaactgg
     2221 tttgttgaaa aagatcactg gaattatttg gataaggata atggtgaacc ttggagacaa
     2281 ttctggttag gtccaagaga tagattgtac agaacgggtg atttaggtcg ttatctacca
     2341 aacggtgact gtgaatgttg cggtagggct gatgatcaag ttaaaattcg tgggttcaga
     2401 atcgaattag gagaaataga tacgcacatt tcccaacatc cattggtaag agaaaacatt
     2461 actttagttc gcaaaaatgc cgacaatgag ccaacattga tcacatttat ggtcccaaga
     2521 tttgacaagc cagatgactt gtctaagttc caaagtgatg ttccaaagga ggttgaaact
     2581 gaccctatag ttaagggctt aatcggttac catcttttat ccaaggacat caggactttc
     2641 ttaaagaaaa gattggctag ctatgctatg ccttccttga ttgtggttat ggataaacta
     2701 ccattgaatc caaatggtaa agttgataag cctaaacttc aattcccaac tcccaagcaa
     2761 ttaaatttgg tagctgaaaa tacagtttct gaaactgacg actctcagtt taccaatgtt
     2821 gagcgcgagg ttagagactt atggttaagt atattaccta ccaagccagc atctgtatca
     2881 ccagatgatt cgtttttcga tttaggtggt cattctatct tggctaccaa aatgattttt
     2941 accttaaaga aaaagctgca agttgattta ccattgggca caattttcaa gtatccaacg
     3001 ataaaggcct ttgccgcgga aattgacaga attaaatcat cgggtggatc atctcaaggt
     3061 gaggtcgtcg aaaatgtcac tgcaaattat gcggaagacg ccaagaaatt ggttgagacg
     3121 ctaccaagtt cgtacccctc tcgagaatat tttgttgaac ctaatagtgc cgaaggaaaa
     3181 acaacaatta atgtgtttgt taccggtgtc acaggatttc tgggctccta catccttgca
     3241 gatttgttag gacgttctcc aaagaactac agtttcaaag tgtttgccca cgtcagggcc
     3301 aaggatgaag aagctgcatt tgcaagatta caaaaggcag gtatcaccta tggtacttgg
     3361 aacgaaaaat ttgcctcaaa tattaaagtt gtattaggcg atttatctaa aagccaattt
     3421 ggtctttcag atgagaagtg gatggatttg gcaaacacag ttgatataat tatccataat
     3481 ggtgcgttag ttcactgggt ttatccatat gccaaattga gggatccaaa tgttatttca
     3541 actatcaatg ttatgagctt agccgccgtc ggcaagccaa agttctttga ctttgtttcc
     3601 tccacttcta ctcttgacac tgaatactac tttaatttgt cagataaact tgttagcgaa
     3661 gggaagccag gcattttaga atcagacgat ttaatgaact ctgcaagcgg gctcactggt
     3721 ggatatggtc agtccaaatg ggctgctgag tacatcatta gacgtgcagg tgaaaggggc
     3781 ctacgtgggt gtattgtcag accaggttac gtaacaggtg cctctgccaa tggttcttca
     3841 aacacagatg atttcttatt gagatttttg aaaggttcag tccaattagg taagattcca
     3901 gatatcgaaa attccgtgaa tatggttcca gtagatcatg ttgctcgtgt tgttgttgct
     3961 acgtctttga atcctcccaa agaaaatgaa ttggccgttg ctcaagtaac gggtcaccca
     4021 agaatattat tcaaagacta cttgtatact ttacacgatt atggttacga tgtcgaaatc
     4081 gaaagctatt ctaaatggaa gaaatcattg gaggcgtctg ttattgacag gaatgaagaa
     4141 aatgcgttgt atcctttgct acacatggtc ttagacaact tacctgaaag taccaaagct
     4201 ccggaactag acgataggaa cgccgtggca tctttaaaga aagacaccgc atggacaggt
     4261 gttgattggt ctaatggaat aggtgttact ccagaagagg ttggtatata tattgcattt
     4321 ttaaacaagg ttggattttt acctccacca actcataatg acaaacttcc actgccaagt
     4381 atagaactaa ctcaagcgca aataagtcta gttgcttcag gtgctggtgc tcgtggaagc
     4441 tccgcagcag cttaaggttg agcattacgt atgatatgtc catgtacaat aattaaatat
     4501 gaattaggag aaagacttag cttcttttcg ggtgatgtca cttaaaaact ccgagaataa
     4561 tatataataa gagaataaaa tattagttat tgaataagaa ctgtaaatca gctggcgtta
     4621 gtctgctaat ggcagcttca tcttggttta ttgtagcatg aatcatattt gccttttttt
     4681 cctgtaattc aatgattctt gcttctatac tatcctcaat gcaaaacctt gtgatcttca
     4741 caggtcgata ctgaccaatt ctatgaactc tatcaccact ttgccattca acactagggt
     4801 tccaccatgg gtctaaaatg aatacttgcg aagctgatct atggtgcact ctcagtacaa
     4861 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4921 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4981 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5041 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5101 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5161 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5221 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5281 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5341 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5401 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5461 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5521 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5581 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5641 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5701 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5761 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5821 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5881 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5941 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6001 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6061 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6121 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6181 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6241 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6361 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6421 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6481 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6541 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6601 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6661 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6721 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6781 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6841 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6901 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6961 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7021 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7081 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7141 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7201 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7261 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     7321 gcgccacctg ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa
     7381 tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt
     7441 gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg
     7501 ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac
     7561 acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa
     7621 tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa
     7681 acctttgttt attttttttt cttcattccg taactcttct accttcttta tttactttct
     7741 aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca
     7801 tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct aagaagcgct
     7861 aagagggcgg ccgctctaga actagtggat cccccgggct gcaggaattc gatatcaagc
     7921 ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg
     7981 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8041 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8101 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     8161 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8221 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8281 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8341 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8401 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8461 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8521 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8581 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcc
//
