LOCUS       sequence-238650-        9663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-HIS3 ICE plasmid used to facilitate yeast recombination cloning
            and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2-HIS3
FEATURES             Location/Qualifiers
     source          1..9663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        109..348
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             349..4527
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(4928..4947)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5047..5069
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5107..5125)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(5297..5959)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(5960..6147)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        6229..6333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6334..6402,6403..7194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6552..6571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7365..7953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7854..7873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8107..8124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8241..8262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8277..8282,8283..8300,8301..8307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8315..8331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8320..8342
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8339..8355
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8339..8355
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8374..8394
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8376..8394
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8449..8952
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8983..8999
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8983..8999
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9033..9049)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9034..9050)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9074..9093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9075..9093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9103..9120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9103..9119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9112..9134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(9260..9663,1..52)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9347..9366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9557..9578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
       61 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagatcaa ttccacttgc
      121 aattacataa aaaattccgg cggtttttcg cgtgtgactc aatgtcgaaa tacctgccta
      181 atgaacatga acatcgccca aatgtatttg aagacccgct gggagaagtt caagatatat
      241 aagtaacaag cagccaatag tataaaaaaa aatctgagtt tattaccttt cctggaattt
      301 cagtgaaaaa ctgctaatta tagagagata tcacagagtt actcactaat gactaacgaa
      361 aaggtctgga tagagaagtt ggataatcca actctttcag tgttaccaca tgacttttta
      421 cgcccacaac aagaacctta tacgaaacaa gctacatatt cgttacagct acctcagctc
      481 gatgtgcctc atgatagttt ttctaacaaa tacgctgtcg ctttgagtgt atgggctgca
      541 ttgatatata gagtaaccgg tgacgatgat attgttcttt atattgcgaa taacaaaatc
      601 ttaagattca atattcaacc aacgtggtca tttaatgagc tgtattctac aattaacaat
      661 gagttgaaca agctcaattc tattgaggcc aatttttcct ttgacgagct agctgaaaaa
      721 attcaaagtt gccaagatct ggaaaggacc cctcagttgt tccgtttggc ctttttggaa
      781 aaccaagatt tcaaattaga cgagttcaag catcatttag tggactttgc tttgaatttg
      841 gataccagta ataatgcgca tgttttgaac ttaatttata acagcttact gtattcgaat
      901 gaaagagtaa ccattgttgc ggaccaattt actcaatatt tgactgctgc gctaagcgat
      961 ccatccaatt gcataactaa aatctctctg atcaccgcat catccaagga tagtttacct
     1021 gatccaacta agaacttggg ctggtgcgat ttcgtggggt gtattcacga cattttccag
     1081 gacaatgctg aagccttccc agagagaacc tgtgttgtgg agactccaac actaaattcc
     1141 gacaagtccc gttctttcac ttatcgcgac atcaaccgca cttctaacat agttgcccat
     1201 tatttgatta aaacaggtat caaaagaggt gatgtagtga tgatctattc ttctaggggt
     1261 gtggatttga tggtatgtgt gatgggtgtc ttgaaagccg gcgcaacctt ttcagttatc
     1321 gaccctgcat atcccccagc cagacaaacc atttacttag gtgttgctaa accacgtggg
     1381 ttgattgtta ttagagctgc tggacaattg gatcaactag tagaagatta catcaatgat
     1441 gaattggaga ttgtttcaag aatcaattcc atcgctattc aagaaaatgg taccattgaa
     1501 ggtggcaaat tggacaatgg cgaggatgtt ttggctccat atgatcacta caaagacacc
     1561 agaacaggtg ttgtagttgg accagattcc aacccaaccc tatctttcac atctggttcc
     1621 gaaggtattc ctaagggtgt tcttggtaga catttttcct tggcttatta tttcaattgg
     1681 atgtccaaaa ggttcaactt aacagaaaat gataaattca caatgctgag cggtattgca
     1741 catgatccaa ttcaaagaga tatgtttaca ccattatttt taggtgccca attgtatgtc
     1801 cctactcaag atgatattgg tacaccgggc cgtttagcgg aatggatgag taagtatggt
     1861 tgcacagtta cccatttaac acctgccatg ggtcaattac ttactgccca agctactaca
     1921 ccattcccta agttacatca tgcgttcttt gtgggtgaca ttttaacaaa acgtgattgt
     1981 ctgaggttac aaaccttggc agaaaattgc cgtattgtta atatgtacgg taccactgaa
     2041 acacagcgtg cagtttctta tttcgaagtt aaatcaaaaa atgacgatcc aaactttttg
     2101 aaaaaattga aagatgtcat gcctgctggt aaaggtatgt tgaacgttca gctactagtt
     2161 gttaacagga acgatcgtac tcaaatatgt ggtattggcg aaataggtga gatttatgtt
     2221 cgtgcaggtg gtttggccga aggttataga ggattaccag aattgaataa agaaaaattt
     2281 gtgaacaact ggtttgttga aaaagatcac tggaattatt tggataagga taatggtgaa
     2341 ccttggagac aattctggtt aggtccaaga gatagattgt acagaacggg tgatttaggt
     2401 cgttatctac caaacggtga ctgtgaatgt tgcggtaggg ctgatgatca agttaaaatt
     2461 cgtgggttca gaatcgaatt aggagaaata gatacgcaca tttcccaaca tccattggta
     2521 agagaaaaca ttactttagt tcgcaaaaat gccgacaatg agccaacatt gatcacattt
     2581 atggtcccaa gatttgacaa gccagatgac ttgtctaagt tccaaagtga tgttccaaag
     2641 gaggttgaaa ctgaccctat agttaagggc ttaatcggtt accatctttt atccaaggac
     2701 atcaggactt tcttaaagaa aagattggct agctatgcta tgccttcctt gattgtggtt
     2761 atggataaac taccattgaa tccaaatggt aaagttgata agcctaaact tcaattccca
     2821 actcccaagc aattaaattt ggtagctgaa aatacagttt ctgaaactga cgactctcag
     2881 tttaccaatg ttgagcgcga ggttagagac ttatggttaa gtatattacc taccaagcca
     2941 gcatctgtat caccagatga ttcgtttttc gatttaggtg gtcattctat cttggctacc
     3001 aaaatgattt ttaccttaaa gaaaaagctg caagttgatt taccattggg cacaattttc
     3061 aagtatccaa cgataaaggc ctttgccgcg gaaattgaca gaattaaatc atcgggtgga
     3121 tcatctcaag gtgaggtcgt cgaaaatgtc actgcaaatt atgcggaaga cgccaagaaa
     3181 ttggttgaga cgctaccaag ttcgtacccc tctcgagaat attttgttga acctaatagt
     3241 gccgaaggaa aaacaacaat taatgtgttt gttaccggtg tcacaggatt tctgggctcc
     3301 tacatccttg cagatttgtt aggacgttct ccaaagaact acagtttcaa agtgtttgcc
     3361 cacgtcaggg ccaaggatga agaagctgca tttgcaagat tacaaaaggc aggtatcacc
     3421 tatggtactt ggaacgaaaa atttgcctca aatattaaag ttgtattagg cgatttatct
     3481 aaaagccaat ttggtctttc agatgagaag tggatggatt tggcaaacac agttgatata
     3541 attatccata atggtgcgtt agttcactgg gtttatccat atgccaaatt gagggatcca
     3601 aatgttattt caactatcaa tgttatgagc ttagccgccg tcggcaagcc aaagttcttt
     3661 gactttgttt cctccacttc tactcttgac actgaatact actttaattt gtcagataaa
     3721 cttgttagcg aagggaagcc aggcatttta gaatcagacg atttaatgaa ctctgcaagc
     3781 gggctcactg gtggatatgg tcagtccaaa tgggctgctg agtacatcat tagacgtgca
     3841 ggtgaaaggg gcctacgtgg gtgtattgtc agaccaggtt acgtaacagg tgcctctgcc
     3901 aatggttctt caaacacaga tgatttctta ttgagatttt tgaaaggttc agtccaatta
     3961 ggtaagattc cagatatcga aaattccgtg aatatggttc cagtagatca tgttgctcgt
     4021 gttgttgttg ctacgtcttt gaatcctccc aaagaaaatg aattggccgt tgctcaagta
     4081 acgggtcacc caagaatatt attcaaagac tacttgtata ctttacacga ttatggttac
     4141 gatgtcgaaa tcgaaagcta ttctaaatgg aagaaatcat tggaggcgtc tgttattgac
     4201 aggaatgaag aaaatgcgtt gtatcctttg ctacacatgg tcttagacaa cttacctgaa
     4261 agtaccaaag ctccggaact agacgatagg aacgccgtgg catctttaaa gaaagacacc
     4321 gcatggacag gtgttgattg gtctaatgga ataggtgtta ctccagaaga ggttggtata
     4381 tatattgcat ttttaaacaa ggttggattt ttacctccac caactcataa tgacaaactt
     4441 ccactgccaa gtatagaact aactcaagcg caaataagtc tagttgcttc aggtgctggt
     4501 gctcgtggaa gctccgcagc agcttaaggt tgagcattac gtatgatatg tccatgtaca
     4561 ataattaaat atgaattagg agaaagactt agcttctttt cgggtgatgt cacttaaaaa
     4621 ctccgagaat aatatataat aagagaataa aatattagtt attgaataag aactgtaaat
     4681 cagctggcgt tagtctgcta atggcagctt catcttggtt tattgtagca tgaatcatat
     4741 ttgccttttt ttcctgtaat tcaatgattc ttgcttctat actatcctca atgcaaaacc
     4801 ttgtgatctt cacaggtcga tactgaccaa ttctatgaac tctatcacca ctttgccatt
     4861 caacactagg gttccaccat gggtctaaaa tgaatacttg cgaagctgat ctatggtgca
     4921 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4981 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5041 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5101 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgatgcct cgttcagaat
     5161 gacacgtata gaatgatgca ttaccttgtc atcttcagta tcatactgtt cgtatacata
     5221 cttactgaca ttcataggta tacatatata cacatgtata tatatcgtat gctgcagctt
     5281 taaataatcg gtgtcactac ataagaacac ctttggtgga gggaacatcg ttggtaccat
     5341 tgggcgaggt ggcttctctt atggcaaccg caagagcctt gaacgcactc tcactacggt
     5401 gatgatcatt cttgcctcgc agacaatcaa cgtggagggt aattctgcta gcctctgcaa
     5461 agctttcaag aaaatgcggg atcatctcgc aagagagatc tcctactttc tccctttgca
     5521 aaccaagttc gacaactgcg tacggcctgt tcgaaagatc taccaccgct ctggaaagtg
     5581 cctcatccaa aggcgcaaat cctgatccaa acctttttac tccacgcacg gcccctaggg
     5641 cctctttaaa agcttgaccg agagcaatcc cgcagtcttc agtggtgtga tggtcgtcta
     5701 tgtgtaagtc accaatgcac tcaacgatta gcgaccagcc ggaatgcttg gccagagcat
     5761 gtatcatatg gtccagaaac cctatacctg tgtggacgtt aatcacttgc gattgtgtgg
     5821 cctgttctgc tactgcttct gcctcttttt ctgggaagat cgagtgctct atcgctaggg
     5881 gaccaccctt taaagagatc gcaatctgaa tcttggtttc atttgtaata cgctttacta
     5941 gggctttctg ctctgtcatc tttgccttcg tttatcttgc ctgctcattt tttagtatat
     6001 tcttcgaaga aatcacatta ctttatataa tgtataattc attatgtgat aatgccaatc
     6061 gctaagaaaa aaaaagagtc atccgctagg tggaaaaaaa aaaatgaaaa tcattaccga
     6121 ggcataaaaa aatatagagt gtactagagg aggccaagag taatagaaaa agaaaattgc
     6181 gggaaaggac tgtgttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6301 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6361 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6421 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6481 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6541 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6601 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6661 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6721 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6781 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6841 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6901 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6961 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7021 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7081 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7141 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7201 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7261 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7321 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7381 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7441 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7501 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7561 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7621 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7681 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7741 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7801 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7861 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7921 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7981 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8041 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8101 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8161 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8221 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8281 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8341 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     8401 aagctggagc tccaccgcgg tggcggccgc gccacctggg tccttttcat cacgtgctat
     8461 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
     8521 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
     8581 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
     8641 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
     8701 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
     8761 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
     8821 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
     8881 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
     8941 aggcaagcga tccgtcctaa gaagcgctaa gagggcggcc gctctagaac tagtggatcc
     9001 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
     9061 ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     9121 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     9181 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     9241 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9301 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9361 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9421 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9481 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9541 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9601 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9661 ctg
//
