LOCUS       sequence-238653-       10515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning
            and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2-LEU2
FEATURES             Location/Qualifiers
     source          1..10515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      71..526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(158..177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     368..389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        583..822
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             823..5001
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5402..5421)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5521..5543
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5581..5599)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(5885..6979)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6980..7387)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        7555..7659
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7660..7728,7729..8520)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7878..7897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8691..9279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9180..9199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9433..9450
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9567..9588
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9603..9608,9609..9626,9627..9633)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9641..9657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9646..9668
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9665..9681
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9665..9681
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9700..9720
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9702..9720
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    9775..10278
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10309..10325
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10309..10325
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10359..10375)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10360..10376)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(10400..10419)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10401..10419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10429..10446)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10429..10445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10438..10460)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
       61 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      121 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      181 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      241 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      301 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      361 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      421 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      481 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
      541 ttctccttac gcatctgtgc ggtatttcac accgcataga tcaattccac ttgcaattac
      601 ataaaaaatt ccggcggttt ttcgcgtgtg actcaatgtc gaaatacctg cctaatgaac
      661 atgaacatcg cccaaatgta tttgaagacc cgctgggaga agttcaagat atataagtaa
      721 caagcagcca atagtataaa aaaaaatctg agtttattac ctttcctgga atttcagtga
      781 aaaactgcta attatagaga gatatcacag agttactcac taatgactaa cgaaaaggtc
      841 tggatagaga agttggataa tccaactctt tcagtgttac cacatgactt tttacgccca
      901 caacaagaac cttatacgaa acaagctaca tattcgttac agctacctca gctcgatgtg
      961 cctcatgata gtttttctaa caaatacgct gtcgctttga gtgtatgggc tgcattgata
     1021 tatagagtaa ccggtgacga tgatattgtt ctttatattg cgaataacaa aatcttaaga
     1081 ttcaatattc aaccaacgtg gtcatttaat gagctgtatt ctacaattaa caatgagttg
     1141 aacaagctca attctattga ggccaatttt tcctttgacg agctagctga aaaaattcaa
     1201 agttgccaag atctggaaag gacccctcag ttgttccgtt tggccttttt ggaaaaccaa
     1261 gatttcaaat tagacgagtt caagcatcat ttagtggact ttgctttgaa tttggatacc
     1321 agtaataatg cgcatgtttt gaacttaatt tataacagct tactgtattc gaatgaaaga
     1381 gtaaccattg ttgcggacca atttactcaa tatttgactg ctgcgctaag cgatccatcc
     1441 aattgcataa ctaaaatctc tctgatcacc gcatcatcca aggatagttt acctgatcca
     1501 actaagaact tgggctggtg cgatttcgtg gggtgtattc acgacatttt ccaggacaat
     1561 gctgaagcct tcccagagag aacctgtgtt gtggagactc caacactaaa ttccgacaag
     1621 tcccgttctt tcacttatcg cgacatcaac cgcacttcta acatagttgc ccattatttg
     1681 attaaaacag gtatcaaaag aggtgatgta gtgatgatct attcttctag gggtgtggat
     1741 ttgatggtat gtgtgatggg tgtcttgaaa gccggcgcaa ccttttcagt tatcgaccct
     1801 gcatatcccc cagccagaca aaccatttac ttaggtgttg ctaaaccacg tgggttgatt
     1861 gttattagag ctgctggaca attggatcaa ctagtagaag attacatcaa tgatgaattg
     1921 gagattgttt caagaatcaa ttccatcgct attcaagaaa atggtaccat tgaaggtggc
     1981 aaattggaca atggcgagga tgttttggct ccatatgatc actacaaaga caccagaaca
     2041 ggtgttgtag ttggaccaga ttccaaccca accctatctt tcacatctgg ttccgaaggt
     2101 attcctaagg gtgttcttgg tagacatttt tccttggctt attatttcaa ttggatgtcc
     2161 aaaaggttca acttaacaga aaatgataaa ttcacaatgc tgagcggtat tgcacatgat
     2221 ccaattcaaa gagatatgtt tacaccatta tttttaggtg cccaattgta tgtccctact
     2281 caagatgata ttggtacacc gggccgttta gcggaatgga tgagtaagta tggttgcaca
     2341 gttacccatt taacacctgc catgggtcaa ttacttactg cccaagctac tacaccattc
     2401 cctaagttac atcatgcgtt ctttgtgggt gacattttaa caaaacgtga ttgtctgagg
     2461 ttacaaacct tggcagaaaa ttgccgtatt gttaatatgt acggtaccac tgaaacacag
     2521 cgtgcagttt cttatttcga agttaaatca aaaaatgacg atccaaactt tttgaaaaaa
     2581 ttgaaagatg tcatgcctgc tggtaaaggt atgttgaacg ttcagctact agttgttaac
     2641 aggaacgatc gtactcaaat atgtggtatt ggcgaaatag gtgagattta tgttcgtgca
     2701 ggtggtttgg ccgaaggtta tagaggatta ccagaattga ataaagaaaa atttgtgaac
     2761 aactggtttg ttgaaaaaga tcactggaat tatttggata aggataatgg tgaaccttgg
     2821 agacaattct ggttaggtcc aagagataga ttgtacagaa cgggtgattt aggtcgttat
     2881 ctaccaaacg gtgactgtga atgttgcggt agggctgatg atcaagttaa aattcgtggg
     2941 ttcagaatcg aattaggaga aatagatacg cacatttccc aacatccatt ggtaagagaa
     3001 aacattactt tagttcgcaa aaatgccgac aatgagccaa cattgatcac atttatggtc
     3061 ccaagatttg acaagccaga tgacttgtct aagttccaaa gtgatgttcc aaaggaggtt
     3121 gaaactgacc ctatagttaa gggcttaatc ggttaccatc ttttatccaa ggacatcagg
     3181 actttcttaa agaaaagatt ggctagctat gctatgcctt ccttgattgt ggttatggat
     3241 aaactaccat tgaatccaaa tggtaaagtt gataagccta aacttcaatt cccaactccc
     3301 aagcaattaa atttggtagc tgaaaataca gtttctgaaa ctgacgactc tcagtttacc
     3361 aatgttgagc gcgaggttag agacttatgg ttaagtatat tacctaccaa gccagcatct
     3421 gtatcaccag atgattcgtt tttcgattta ggtggtcatt ctatcttggc taccaaaatg
     3481 atttttacct taaagaaaaa gctgcaagtt gatttaccat tgggcacaat tttcaagtat
     3541 ccaacgataa aggcctttgc cgcggaaatt gacagaatta aatcatcggg tggatcatct
     3601 caaggtgagg tcgtcgaaaa tgtcactgca aattatgcgg aagacgccaa gaaattggtt
     3661 gagacgctac caagttcgta cccctctcga gaatattttg ttgaacctaa tagtgccgaa
     3721 ggaaaaacaa caattaatgt gtttgttacc ggtgtcacag gatttctggg ctcctacatc
     3781 cttgcagatt tgttaggacg ttctccaaag aactacagtt tcaaagtgtt tgcccacgtc
     3841 agggccaagg atgaagaagc tgcatttgca agattacaaa aggcaggtat cacctatggt
     3901 acttggaacg aaaaatttgc ctcaaatatt aaagttgtat taggcgattt atctaaaagc
     3961 caatttggtc tttcagatga gaagtggatg gatttggcaa acacagttga tataattatc
     4021 cataatggtg cgttagttca ctgggtttat ccatatgcca aattgaggga tccaaatgtt
     4081 atttcaacta tcaatgttat gagcttagcc gccgtcggca agccaaagtt ctttgacttt
     4141 gtttcctcca cttctactct tgacactgaa tactacttta atttgtcaga taaacttgtt
     4201 agcgaaggga agccaggcat tttagaatca gacgatttaa tgaactctgc aagcgggctc
     4261 actggtggat atggtcagtc caaatgggct gctgagtaca tcattagacg tgcaggtgaa
     4321 aggggcctac gtgggtgtat tgtcagacca ggttacgtaa caggtgcctc tgccaatggt
     4381 tcttcaaaca cagatgattt cttattgaga tttttgaaag gttcagtcca attaggtaag
     4441 attccagata tcgaaaattc cgtgaatatg gttccagtag atcatgttgc tcgtgttgtt
     4501 gttgctacgt ctttgaatcc tcccaaagaa aatgaattgg ccgttgctca agtaacgggt
     4561 cacccaagaa tattattcaa agactacttg tatactttac acgattatgg ttacgatgtc
     4621 gaaatcgaaa gctattctaa atggaagaaa tcattggagg cgtctgttat tgacaggaat
     4681 gaagaaaatg cgttgtatcc tttgctacac atggtcttag acaacttacc tgaaagtacc
     4741 aaagctccgg aactagacga taggaacgcc gtggcatctt taaagaaaga caccgcatgg
     4801 acaggtgttg attggtctaa tggaataggt gttactccag aagaggttgg tatatatatt
     4861 gcatttttaa acaaggttgg atttttacct ccaccaactc ataatgacaa acttccactg
     4921 ccaagtatag aactaactca agcgcaaata agtctagttg cttcaggtgc tggtgctcgt
     4981 ggaagctccg cagcagctta aggttgagca ttacgtatga tatgtccatg tacaataatt
     5041 aaatatgaat taggagaaag acttagcttc ttttcgggtg atgtcactta aaaactccga
     5101 gaataatata taataagaga ataaaatatt agttattgaa taagaactgt aaatcagctg
     5161 gcgttagtct gctaatggca gcttcatctt ggtttattgt agcatgaatc atatttgcct
     5221 ttttttcctg taattcaatg attcttgctt ctatactatc ctcaatgcaa aaccttgtga
     5281 tcttcacagg tcgatactga ccaattctat gaactctatc accactttgc cattcaacac
     5341 tagggttcca ccatgggtct aaaatgaata cttgcgaagc tgatctatgg tgcactctca
     5401 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5461 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5521 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5581 gcctcgtgat acgcctattt ttataggtta atgtcatgat ctttttgtgt ggtgccctcc
     5641 tccttgtcaa tattaatgtt aaagtgcaat tctttttcct tatcacgttg agccattagt
     5701 atcaatttgc ttacctgtat tcctttacat cctccttttt ctccttcttg ataaatgtat
     5761 gtagattgcg tatatagttt cgtctaccct atgaacatat tccattttgt aatttcgtgt
     5821 cgtttctatt atgaatttca tttataaagt ttatgtacaa atatcataaa aaaagagaat
     5881 ctttttaagc aaggattttc ttaacttctt cggcgacagc atcaccgact tcggtggtac
     5941 tgttggaacc acctaaatca ccagttctga tacctgcatc caaaaccttt ttaactgcat
     6001 cttcaatggc cttaccttct tcaggcaagt tcaatgacaa tttcaacatc attgcagcag
     6061 acaagatagt ggcgataggg ttgaccttat tctttggcaa atctggagca gaaccgtggc
     6121 atggttcgta caaaccaaat gcggtgttct tgtctggcaa agaggccaag gacgcagatg
     6181 gcaacaaacc caaggaacct gggataacgg aggcttcatc ggagatgata tcaccaaaca
     6241 tgttgctggt gattataata ccatttaggt gggttgggtt cttaactagg atcatggcgg
     6301 cagaatcaat caattgatgt tgaaccttca atgtagggaa ttcgttcttg atggtttcct
     6361 ccacagtttt tctccataat cttgaagagg ccaaaacatt agctttatcc aaggaccaaa
     6421 taggcaatgg tggctcatgt tgtagggcca tgaaagcggc cattcttgtg attctttgca
     6481 cttctggaac ggtgtattgt tcactatccc aagcgacacc atcaccatcg tcttcctttc
     6541 tcttaccaaa gtaaatacct cccactaatt ctctgacaac aacgaagtca gtacctttag
     6601 caaattgtgg cttgattgga gataagtcta aaagagagtc ggatgcaaag ttacatggtc
     6661 ttaagttggc gtacaattga agttctttac ggatttttag taaaccttgt tcaggtctaa
     6721 cactaccggt accccattta ggaccaccca cagcacctaa caaaacggca tcagccttct
     6781 tggaggcttc cagcgcctca tctggaagtg gaacacctgt agcatcgata gcagcaccac
     6841 caattaaatg attttcgaaa tcgaacttga cattggaacg aacatcagaa atagctttaa
     6901 gaaccttaat ggcttcggct gtgatttctt gaccaacgtg gtcacctggc aaaacgacga
     6961 tcttcttagg ggcagacatt agaatggtat atccttgaaa tatatatata tatattgctg
     7021 aaatgtaaaa ggtaagaaaa gttagaaagt aagacgattg ctaaccacct attggaaaaa
     7081 acaataggtc cttaaataat attgtcaact tcaagtattg tgatgcaagc atttagtcat
     7141 gaacgcttct ctattctata tgaaaagccg gttccggcgc tctcaccttt cctttttctc
     7201 ccaatttttc agttgaaaaa ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt
     7261 tccagtcatc gaatttgatt ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg
     7321 aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc ttacgatacc tgagtattcc
     7381 cacagttaac tgcggtcaag atatttcttg aatcaggcgc cttagaccgc tcggccaaac
     7441 aaccaattac ttgttgagaa atagagtata attatcctat aaatataacg tttttgaaca
     7501 cacatgaaca aggaagtaca ggagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7561 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7621 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7681 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7741 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7801 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7861 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     7921 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7981 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8041 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8101 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8161 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8221 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8281 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8341 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8401 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8461 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8521 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8581 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8641 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8701 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8761 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8821 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8881 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8941 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9001 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9061 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9121 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9181 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9241 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9301 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9361 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9421 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9481 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9541 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9601 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9661 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     9721 gaacaaaagc tggagctcca ccgcggtggc ggccgcgcca cctgggtcct tttcatcacg
     9781 tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa
     9841 agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta
     9901 gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc
     9961 cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt
    10021 ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata
    10081 tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat
    10141 tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat
    10201 aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta
    10261 agttacaggc aagcgatccg tcctaagaag cgctaagagg gcggccgctc tagaactagt
    10321 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg
    10381 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
    10441 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    10501 tccccctttc gccag
//
